8EAT: Yeast VO missing subunits a, e, and f

Yeast VO missing subunits a, e, and f in complex with Vma12-22p. Determined by electron microscopy at 3.1 Å resolution. Released 2 Nov 2022.

Method
Electron microscopy
Resolution
3.1 Å
Organism
Saccharomyces cerevisiae
Chains
15
Atoms
17,200
Mol. weight
299.94 kDa
Released
2 Nov 2022

Explore 8EAT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8EAT contains 99 α-helices and 11 β-strands across 15 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 3 β-strands

ElementResiduesLengthSheet
α-helix16-4934
α-helix52-543
β-strand7011
β-strand73-7752
β-strand86-9052
α-helix132-1365
α-helix144-17633
Chain b: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix221-24525
Chain B: 5 helices, 3 β-strands
ElementResiduesLengthSheet
β-strand2-543
α-helix8-1811
α-helix25-3713
β-strand39-4023
α-helix42-5211
β-strand71-7333
α-helix74-785
α-helix85-10420
Chain c: 9 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix17-3822
α-helix42-443
α-helix47-537
α-helix56-9035
α-helix96-12227
α-helix127-1293
α-helix136-17641
α-helix178-20528
α-helix210-2123
Chain d: 20 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix2-65
α-helix11-2212
α-helix25-262
α-helix27-359
α-helix39-468
α-helix65-8521
α-helix90-11526
α-helix120-1234
α-helix136-1394
α-helix148-1503
α-helix153-1553
α-helix159-1624
α-helix174-19926
α-helix202-22625
α-helix235-2417
α-helix251-2588
α-helix262-2709
α-helix284-29916
α-helix307-33226
α-helix335-3373
Chain F: 6 helices, 5 β-strands
ElementResiduesLengthSheet
β-strand7-1264
α-helix14-2310
β-strand2811
β-strand37-3934
α-helix47-5913
β-strand64-7074
α-helix71-766
α-helix78-836
β-strand90-9454
α-helix103-1053
α-helix107-1137
Chain g: 4 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix9-4335
α-helix55-7319
α-helix84-12138
α-helix125-15329
Chain h: 6 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix9-4234
α-helix47-493
α-helix50-534
α-helix55-7521
α-helix84-12138
α-helix125-15329

7 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Vacuolar ATPase assembly protein VMA22Aprotein181Saccharomyces cerevisiaeP38784 (AlphaFold model)
Vacuolar ATPase assembly integral membrane protein VPH2Bprotein215Saccharomyces cerevisiaeP32341 (AlphaFold model)
V-type proton ATPase subunit FFprotein118Saccharomyces cerevisiaeP39111 (AlphaFold model)
V0 assembly protein 1bprotein265Saccharomyces cerevisiaeP53262 (AlphaFold model)
V-type proton ATPase subunit c''cprotein213Saccharomyces cerevisiaeP23968
V-type proton ATPase subunit ddprotein345Saccharomyces cerevisiaeP32366
V-type proton ATPase subunit cg, h, i, j, k, l, m, nprotein160Saccharomyces cerevisiaeP25515
V-type proton ATPase subunit c'oprotein164Saccharomyces cerevisiaeP32842
Sequence of entity 1 (A), FASTA
>8EAT_1 Vacuolar ATPase assembly protein VMA22 (chains A)
MSETRMAQNMDTTDEQYLRLIELLSNYDSTLEQLQKGFQDGYIQLSRSNYYNKDSLRGNY
GEDYWDETYIGQLMATVEEKNSKVVVEIVKRKAQDKQEKKEEEDNKLTQRKKGTKPEKQK
TQSHKLKQDYDPILMFGGVLSVPSSLRQSQTSFKGCIPLIAQLINYKNEILTLVETLSEQ
E
Sequence of entity 2 (B), FASTA
>8EAT_2 Vacuolar ATPase assembly integral membrane protein VPH2 (chains B)
MFEIKLNDRITEFLRKFKNSAKSNEGIDEDIDLFLKRHAIPMQSLLFYVKEYRKDSDLQC
SIKELLKPLEFEFKPKAVRGLHYSEDFKKKLEFLKYQEQELEYQSMVKRSKSVFSLQEDD
ELTPSQINKQIKEQVTTVFNVLVSVISVVVAIWYWTGSSTNFPVHVRLLLCLFFGILVLV
ADVVVYNSYLKKLEEAKVKEKTKVEKKKVLSKITL
Sequence of entity 3 (F), FASTA
>8EAT_3 V-type proton ATPase subunit F (chains F)
MAEKRTLIAVIADEDTTTGLLLAGIGQITPETQEKNFFVYQEGKTTKEEITDKFNHFTEE
RDDIAILLINQHIAENIRARVDSFTNAFPAILEIPSKDHPYDPEKDSVLKRVRKLFGE
Sequence of entity 4 (b), FASTA
>8EAT_4 V0 assembly protein 1 (chains b)
MVFGQLYALFIFTLSCCISKTVQADSSKESSSFISFDKESNWDTISTISSTADVISSVDS
AIAVFEFDNFSLLDNLMIDEEYPFFNRFFANDVSLTVHDDSPLNISQSLSPIMEQFTVDE
LPESASDLLYEYSLDDKSIVLFKFTSDAYDLKKLDEFIDSCLSFLEDKSGDNLTVVINSL
GWAFEDEDGDDEYATEETLSHHDNNKGKEGDDDILSSIWTEGLLMCLIVSALLLFILIVA
LSWISNLDITYGALEKSTNPIKKNN
Sequence of entity 5 (c), FASTA
>8EAT_5 V-type proton ATPase subunit c'' (chains c)
MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSDINFGKFLLRTSPYMWA
NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIV
FSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSAL
FVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ
Sequence of entity 6 (d), FASTA
>8EAT_6 V-type proton ATPase subunit d (chains d)
MEGVYFNIDNGFIEGVVRGYRNGLLSNNQYINLTQCDTLEDLKLQLSSTDYGNFLSSVSS
ESLTTSLIQEYASSKLYHEFNYIRDQSSGSTRKFMDYITYGYMIDNVALMITGTIHDRDK
GEILQRCHPLGWFDTLPTLSVATDLESLYETVLVDTPLAPYFKNCFDTAEELDDMNIEII
RNKLYKAYLEDFYNFVTEEIPEPAKECMQTLLGFEADRRSINIALNSLQSSDIDPDLKSD
LLPNIGKLYPLATFHLAQAQDFEGVRAALANVYEYRGFLETGNLEDHFYQLEMELCRDAF
TQQFAISTVWAWMKSKEQEVRNITWIAECIAQNQRERINNYISVY
Sequence of entity 7 (g, h, i, j, k, l, m, n), FASTA
>8EAT_7 V-type proton ATPase subunit c (chains g, h, i, j, k, l, m, n)
MTELCPVYAPFFGAIGCASAIIFTSLGAAYGTAKSGVGICATCVLRPDLLFKNIVPVIMA
GIIAIYGLVVSVLVCYSLGQKQALYTGFIQLGAGLSVGLSGLAAGFAIGIVGDAGVRGSS
QQPRLFVGMILILIFAEVLGLYGLIVALLLNSRATQDVVC
Sequence of entity 8 (o), FASTA
>8EAT_8 V-type proton ATPase subunit c' (chains o)
MSTQLASNIYAPLYAPFFGFAGCAAAMVLSCLGAAIGTAKSGIGIAGIGTFKPELIMKSL
IPVVMSGILAIYGLVVAVLIAGNLSPTEDYTLFNGFMHLSCGLCVGFACLSSGYAIGMVG
DVGVRKYMHQPRLFVGIVLILIFSEVLGLYGMIVALILNTRGSE

Primary citation

Structural basis of V-ATPase V O region assembly by Vma12p, 21p, and 22p. Wang, H., Bueler, S.A., Rubinstein, J.L. Proc Natl Acad Sci U S A (2023) 120:e2217181120-e2217181120. DOI 10.1073/pnas.2217181120 · PubMed

Other PDB entries of the same protein (UniProt P38784 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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