8EOI: ER membrane protein complex subunit 1
Structure of a human EMC:human Cav1.2 channel complex in GDN detergent. Determined by electron microscopy at 3.4 Å resolution. Released 24 May 2023.
- Method
- Electron microscopy
- Resolution
- 3.4 Å
- Organisms
- Homo sapiens, Oryctolagus cuniculus
- Chains
- 11
- Atoms
- 28,721
- Mol. weight
- 483.94 kDa
- Ligands
- NAG, 9Z9
- Released
- 24 May 2023
Explore 8EOI in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8EOI contains 131 α-helices and 121 β-strands across 11 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 21 helices, 70 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 32-34 | 3 | 1 |
| β-strand | 40-44 | 5 | 2 |
| β-strand | 54-58 | 5 | 2 |
| β-strand | 62-66 | 5 | 2 |
| β-strand | 73-78 | 6 | 2 |
| α-helix | 83-85 | 3 | |
| β-strand | 87-93 | 7 | 3 |
| β-strand | 96-101 | 6 | 3 |
| β-strand | 106-111 | 6 | 3 |
| β-strand | 119-122 | 4 | 3 |
| β-strand | 128-135 | 8 | 4 |
| β-strand | 140-147 | 8 | 4 |
| β-strand | 150-155 | 6 | 4 |
| β-strand | 161-166 | 6 | 4 |
| α-helix | 167 | 1 | |
| β-strand | 174-180 | 7 | 5 |
| β-strand | 185-191 | 7 | 5 |
| β-strand | 195-203 | 9 | 5 |
| β-strand | 209-217 | 9 | 5 |
| β-strand | 228-230 | 3 | 6 |
| β-strand | 234-239 | 6 | 6 |
| β-strand | 244-249 | 6 | 6 |
| β-strand | 257-260 | 4 | 6 |
| α-helix | 268-269 | 2 | |
| β-strand | 275-277 | 3 | 7 |
| α-helix | 286-288 | 3 | |
| β-strand | 290-296 | 7 | 7 |
| β-strand | 299-306 | 8 | 7 |
| β-strand | 309-317 | 9 | 7 |
| β-strand | 321-327 | 7 | 8 |
| β-strand | 330-338 | 9 | 8 |
| β-strand | 372-379 | 8 | 8 |
| β-strand | 384-385 | 2 | 8 |
| α-helix | 386 | 1 | |
| β-strand | 391-394 | 4 | 8 |
| β-strand | 404-411 | 8 | 9 |
| β-strand | 417-424 | 8 | 9 |
| β-strand | 429-433 | 5 | 9 |
| β-strand | 441-446 | 6 | 9 |
| α-helix | 447-450 | 4 | |
| β-strand | 452-459 | 8 | 10 |
| α-helix | 460-463 | 4 | |
| α-helix | 464-467 | 4 | |
| α-helix | 469-472 | 4 | |
| α-helix | 480-503 | 24 | |
| α-helix | 522-524 | 3 | |
| β-strand | 534-540 | 7 | 10 |
| β-strand | 544-549 | 6 | 10 |
| β-strand | 555-560 | 6 | 10 |
| β-strand | 569-576 | 8 | 11 |
| β-strand | 586-592 | 7 | 11 |
| β-strand | 599-604 | 6 | 11 |
| α-helix | 610 | 1 | |
| β-strand | 611 | 1 | 11 |
| α-helix | 612 | 1 | |
| α-helix | 615-616 | 2 | |
| β-strand | 623-632 | 10 | 12 |
| β-strand | 638-644 | 7 | 12 |
| β-strand | 648 | 1 | 13 |
| β-strand | 649-652 | 4 | 12 |
| α-helix | 656-665 | 10 | |
| α-helix | 666-668 | 3 | |
| β-strand | 669-675 | 7 | 14 |
| β-strand | 680-686 | 7 | 14 |
| β-strand | 691 | 1 | 13 |
| β-strand | 692-699 | 8 | 14 |
| β-strand | 705-711 | 7 | 15 |
| β-strand | 723-725 | 3 | 16 |
| β-strand | 731-733 | 3 | 16 |
| β-strand | 734 | 1 | 17 |
| β-strand | 740-747 | 8 | 15 |
| β-strand | 756-763 | 8 | 15 |
| β-strand | 769-776 | 8 | 15 |
| β-strand | 778-787 | 10 | 17 |
| β-strand | 790-797 | 8 | 17 |
| β-strand | 802-811 | 10 | 17 |
| β-strand | 822 | 1 | 18 |
| β-strand | 832-840 | 9 | 17 |
| β-strand | 846-849 | 4 | 19 |
| α-helix | 858-859 | 2 | |
| β-strand | 860-864 | 5 | 19 |
| β-strand | 870-874 | 5 | 19 |
| α-helix | 888-893 | 6 | |
| α-helix | 896-898 | 3 | |
| β-strand | 901 | 1 | 17 |
| α-helix | 906-908 | 3 | |
| β-strand | 909 | 1 | 19 |
| β-strand | 921-925 | 5 | 1 |
| β-strand | 933-938 | 6 | 1 |
| β-strand | 942-947 | 6 | 1 |
| α-helix | 962-991 | 30 | |
Chain B: 15 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4 | 1 | 20 |
| β-strand | 8 | 1 | 20 |
| α-helix | 12-24 | 13 | |
| α-helix | 30-43 | 14 | |
| α-helix | 52-65 | 14 | |
| α-helix | 69-82 | 14 | |
| α-helix | 87-99 | 13 | |
| α-helix | 103-116 | 14 | |
| α-helix | 122-133 | 12 | |
| α-helix | 137-150 | 14 | |
| α-helix | 155-167 | 13 | |
| α-helix | 171-184 | 14 | |
| α-helix | 189-202 | 14 | |
| α-helix | 205-221 | 17 | |
| α-helix | 226-240 | 15 | |
| α-helix | 247-274 | 28 | |
| α-helix | 281-290 | 10 | |
Chain C: 12 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-12 | 3 | |
| α-helix | 13-17 | 5 | |
| α-helix | 18-37 | 20 | |
| α-helix | 46-64 | 19 | |
| α-helix | 65-67 | 3 | |
| α-helix | 70-78 | 9 | |
| α-helix | 115-132 | 18 | |
| β-strand | 138-139 | 2 | 21 |
| α-helix | 149-152 | 4 | |
| β-strand | 165-166 | 2 | 21 |
| α-helix | 168-177 | 10 | |
| α-helix | 180-186 | 7 | |
| α-helix | 214-226 | 13 | |
| α-helix | 237-242 | 6 | |
Chain D: 1 helix, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 165-167 | 3 | |
| β-strand | 173 | 1 | 18 |
| β-strand | 176-181 | 6 | 14 |
Chain E: 5 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-34 | 30 | |
| α-helix | 43-60 | 18 | |
| β-strand | 71 | 1 | 22 |
| α-helix | 81-84 | 4 | |
| α-helix | 88-90 | 3 | |
| α-helix | 97-100 | 4 | |
Chain F: 5 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| β-strand | 14 | 1 | 22 |
| α-helix | 16-44 | 29 | |
| α-helix | 49-70 | 22 | |
| α-helix | 74-76 | 3 | |
| α-helix | 82-85 | 4 | |
| α-helix | 91-107 | 17 | |
Chain G: 2 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 45-52 | 8 | 23 |
| α-helix | 58-60 | 3 | |
| α-helix | 62-64 | 3 | |
| β-strand | 66-69 | 4 | 24 |
| β-strand | 74-76 | 3 | 24 |
| β-strand | 78 | 1 | 23 |
| β-strand | 84-89 | 6 | 23 |
| β-strand | 92-99 | 8 | 24 |
| β-strand | 108-112 | 5 | 24 |
| β-strand | 118-121 | 4 | 24 |
| β-strand | 133-134 | 2 | 24 |
| β-strand | 138-141 | 4 | 23 |
Chain H: 7 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 6 | 1 | 25 |
| α-helix | 8-20 | 13 | |
| β-strand | 26-33 | 8 | 26 |
| β-strand | 54-62 | 9 | 26 |
| α-helix | 68-84 | 17 | |
| β-strand | 87-95 | 9 | 26 |
| α-helix | 106-116 | 11 | |
| β-strand | 123-127 | 5 | 26 |
| β-strand | 141-147 | 7 | 26 |
| β-strand | 150-153 | 4 | 26 |
| β-strand | 161 | 1 | 25 |
| α-helix | 164-176 | 13 | |
| α-helix | 179-181 | 3 | |
| α-helix | 186-191 | 6 | |
| α-helix | 200-208 | 9 | |
3 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| ER membrane protein complex subunit 1 | A | protein | 973 | Homo sapiens | Q8N766 (AlphaFold model) |
| ER membrane protein complex subunit 2 | B | protein | 291 | Homo sapiens | Q15006 (AlphaFold model) |
| ER membrane protein complex subunit 3 | C | protein | 258 | Homo sapiens | Q9P0I2 (AlphaFold model) |
| ER membrane protein complex subunit 5 | E | protein | 101 | Homo sapiens | Q8N4V1 (AlphaFold model) |
| ER membrane protein complex subunit 6 | F | protein | 100 | Homo sapiens | Q9BV81 |
| ER membrane protein complex subunit 7 | G | protein | 116 | Homo sapiens | Q9NPA0 |
| ER membrane protein complex subunit 8 | H | protein | 208 | Homo sapiens | O43402 |
| ER membrane protein complex subunit 10 | I | protein | 157 | Homo sapiens | Q5UCC4 |
| Voltage-dependent L-type calcium channel subunit alpha-1C | K | protein | 1550 | Homo sapiens | Q13936 |
| Voltage-dependent L-type calcium channel subunit beta-3 | J | protein | 324 | Oryctolagus cuniculus | P54286 |
| ER membrane protein complex subunit 4 | D | protein | 170 | Homo sapiens | Q5J8M3 |
Sequence of entity 1 (A), FASTA
>8EOI_1 ER membrane protein complex subunit 1 (chains A)
AVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDK
GTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESV
RYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIV
KFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIP
LQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTA
LVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLV
ETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKV
VLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTS
HLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQY
LPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLK
RPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGR
LCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNL
LAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKA
RRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRH
LLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMR
GIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITK
RLAQVKLLNRAWR
Sequence of entity 2 (B), FASTA
>8EOI_2 ER membrane protein complex subunit 2 (chains B)
KVSELYDVTWEEMRDKMRKWREENSRNSEQIVEVGEELINEYASKLGDDIWIIYEQVMIA
ALDYGRDDLALFCLQELRRQFPGSHRVKRLTGMRFEAMERYDDAIQLYDRILQEDPTNTA
ARKRKIAIRKAQGKNVEAIRELNEYLEQFVGDQEAWHELAELYINEHDYAKAAFCLEELM
MTNPHNHLYCQQYAEVKYTQGGLENLELSRKYFAQALKLNNRNMRALFGLYMSASHIASN
PKASAKTKKDNMKYASWAASQINRAYQFAGRSKKETKYSLKAVEDMLETLQ
Sequence of entity 3 (C), FASTA
>8EOI_3 ER membrane protein complex subunit 3 (chains C)
PELLLDSNIRLWVVLPIVIITFFVGMIRHYVSILLQSDKKLTQEQVSDSQVLIRSRVLRE
NGKYIPKQSFLTRKYYFNNPEDGFFKKTKRKVVPPSPMTDPTMLTDMMKGNVTNVLPMIL
IGGWINMTFSGFVTTKVPFPLTLRFKPMLQQGIELLTLDASWVSSASWYFLNVFGLRSIY
SLILGQDNAADQSRMMQEQMTGAAMAMPADTNKAFKTEWEALELTDHQWALDDVEEELMA
KDLHFEGMFKKELQTSIF
Sequence of entity 4 (E), FASTA
>8EOI_4 ER membrane protein complex subunit 5 (chains E)
PSLWKGLVGIGLFALAHAAFSAAQHRSYMRLTEKEDESLPIDIVLQTLLAFAVTCYGIVH
IAGEFKDMDATSELKNKTFDTLRNHPSFYVFNHRGRVLFRP
Sequence of entity 5 (F), FASTA
>8EOI_5 ER membrane protein complex subunit 6 (chains F)
PPFISEAAVRGNAAVLDYCRTSVSALSGATAGILGLTGLYGFIFYLLASVLLSLLLILKA
GRRWNKYFKSRRPLFTGGLIGGLFTYVLFWTFLYGMVHVY
Sequence of entity 6 (G), FASTA
>8EOI_6 ER membrane protein complex subunit 7 (chains G)
DRFKIEGRAVVPGVKPQDWISAARVLVDGEEHVGFLKTDGSFVVHDIPSGSYVVEVVSPA
YRFDPVRVDITSKGKMRARYVNYIKTSEVVRLPYPLQMKSSGPPSYFIKRESWGWT
Sequence of entity 7 (H), FASTA
>8EOI_7 ER membrane protein complex subunit 8 (chains H)
GVKLTTQAYCKMVLHGAKYPHCAVNGLLVAEKQKPRKEHLPLGGPGAHHTLFVDCIPLFH
GTLALAPMLEVALTLIDSWCKDHSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFSDT
ALIMVDNTKFTMDCVAPTIHVYEHHENRWRCRDPHHDYCEDWPEAQRISASLLDSRSYET
LVDFDNHLDDIRNDWTNPEINKAVLHLC
Sequence of entity 8 (I), FASTA
>8EOI_8 ER membrane protein complex subunit 10 (chains I)
GTVGLLLEHSFEIDDSANFRKRGSLLWNQQDGTLSLSQRQLSEEERGRLRDVAALNGLYR
VRIPRRPGALDGLEAGGYVSSFVPACSLVESHLSDQLTLHVDVAGNVVGVSVVTHPGGCR
GHEVEDVDLELFNTSVQLQPPTTAPGPETAAFIERLE
Sequence of entity 9 (K), FASTA
>8EOI_9 Voltage-dependent L-type calcium channel subunit alpha-1C (chains K)
LKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLI
IFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKG
AGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLE
LFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDN
FAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEF
SKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPT
SETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYW
LVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLF
NRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSV
RSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDW
NSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKE
EEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQP
NENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNR
FRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEI
ALKILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQS
SAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGV
QLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMAL
FTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVT
FQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVL
ILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDF
LIVIGSIIDVILSETNHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEEN
SRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQ
VFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNS
TEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRI
WAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNA
TLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGD
Sequence of entity 10 (J), FASTA
>8EOI_10 Voltage-dependent L-type calcium channel subunit beta-3 (chains J)
ESARREVESQAQQQLERAKHKPVAFAVRTNVSYCGVLDEECPVQGSGINFEAKDFLHIKE
KYSNDWWIGRLVKEGGDIAFIPSPQRLESIRLKQEQKARRSGNPSSLSDIGNRRSPPPSL
AKQKQKQAEHVPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRV
TADLSLAKRSVLNNPGKRTIIERSSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHP
AQLAKTSLAPIIVFVKVSSPKVLQRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVIL
DENQLEDACEHLAEYLEVYWRATH
Sequence of entity 11 (D), FASTA
>8EOI_11 ER membrane protein complex subunit 4 (chains D)
RFKWAIELSGPGGGSRGRSDRGSGQGDSLYPVGYLDKQVPDTSVQETDRILVEKRCWDIA
LGPLKQIPMNLFIMYMAGNTISIFPTMMVCMMAWRPIQALMAISATFKMLESSSQKFLQG
LVYLIGNLMGLALAVYKCQSMGLLPTHASDWLAFIEPPERMEFSGGGLLL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 1 |
| 9Z9 | (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en | C34 H56 O5 | 1 |
Primary citation
EMC chaperone-Ca V structure reveals an ion channel assembly intermediate. Chen, Z., Mondal, A., Abderemane-Ali, F. et al. Nature (2023) 619:410-419. DOI 10.1038/s41586-023-06175-5 · PubMed
Other PDB entries of the same protein (UniProt Q8N766 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8J0O 3.32 Å, cryo-EM structure of human EMC and VDAC
- 7ADO 3.39 Å, Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs
- 6WW7 3.4 Å, Structure of the human ER membrane protein complex in a lipid nanodisc
- 8J0N 3.47 Å, cryo-EM structure of human EMC
- 7ADP 3.6 Å, Cryo-EM structure of human ER membrane protein complex in GDN detergent
- 8S9S 3.6 Å, Structure of the human ER membrane protein complex (EMC) in GDN
- 9ZZ6 4.16 Å, The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium…
- 6Z3W 6.4 Å, Human ER membrane protein complex
- 9C7V 6.6 Å, Structure of the human BOS:human EMC complex in GDN
Browse structure collections
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