8GIY: E. coli clamp loader with closed clamp

E. coli clamp loader with closed clamp. Determined by electron microscopy at 3.7 Å resolution. Released 27 Mar 2024.

Method: Electron microscopy
Resolution: 3.7 Å
Organism: Escherichia coli K-12
Chains: 8
Atoms: 19,865
Mol. weight: 316.88 kDa
Ligands: ZN, MG, AGS
Released: 27 Mar 2024

Explore 8GIY in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8GIY contains 144 α-helices and 88 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 20 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	2-4	3	1
α-helix	6-15	10
β-strand	20-25	6	1
α-helix	28-45	18
β-strand	49-54	6	1
α-helix	61-69	9
β-strand	78-83	6	1
α-helix	91-102	12
β-strand	109-114	6	1
α-helix	116-117	2
α-helix	119-122	4
α-helix	125-129	5
α-helix	131-133	3
β-strand	134-139	6	1
α-helix	141-143	3
α-helix	147-157	11
β-strand	161-162	2	2
α-helix	164-173	10
α-helix	178-191	14
β-strand	196-197	2	2
α-helix	199-206	8
α-helix	214-222	9
α-helix	226-239	14
α-helix	243-263	21
α-helix	269-275	7
α-helix	280-290	11
α-helix	300-314	15
α-helix	320-331	12

Chain B: 24 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-9	4
α-helix	22-34	13
β-strand	40-44	5	3
α-helix	51-62	12
α-helix	77-84	8
β-strand	90-94	5	3
α-helix	101-108	8
α-helix	109-112	4
α-helix	114-115	2
β-strand	121-126	6	3
α-helix	128-130	3
α-helix	133-144	12
β-strand	150-155	6	3
α-helix	159-161	3
α-helix	164-168	5
β-strand	171-174	4	3
α-helix	181-194	14
α-helix	200-210	11
α-helix	214-228	15
α-helix	234-243	10
α-helix	248-257	10
α-helix	261-273	13
α-helix	278-297	20
α-helix	299-301	3
α-helix	304-306	3
α-helix	310-319	10
α-helix	322-338	17
α-helix	339-341	3
α-helix	345-358	14
β-strand	359-362	4	4

Chain C: 23 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	6-9	4
α-helix	15-17	3
α-helix	22-34	13
β-strand	40-44	5	5
α-helix	51-63	13
α-helix	77-84	8
β-strand	90-94	5	5
α-helix	101-108	8
α-helix	114-115	2
β-strand	121-126	6	5
α-helix	128-130	3
α-helix	133-144	12
β-strand	150-156	7	5
α-helix	159-161	3
α-helix	164-167	4
β-strand	171-174	4	5
α-helix	180-194	15
α-helix	200-209	10
α-helix	214-228	15
α-helix	234-241	8
α-helix	246-258	13
α-helix	261-273	13
α-helix	278-297	20
α-helix	299-301	3
α-helix	307-317	11
α-helix	322-338	17
α-helix	345-358	14
α-helix	364-368	5

Chain D: 24 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	6-9	4
α-helix	15-17	3
α-helix	22-34	13
β-strand	40-44	5	6
α-helix	51-62	12
α-helix	77-83	7
β-strand	90-94	5	6
α-helix	101-109	9
α-helix	114-115	2
β-strand	121-126	6	6
α-helix	128-130	3
α-helix	133-144	12
β-strand	150-155	6	6
α-helix	159-161	3
α-helix	164-168	5
β-strand	171-174	4	6
α-helix	175-179	5
α-helix	180-194	15
α-helix	200-210	11
α-helix	214-227	14
α-helix	234-240	7
α-helix	245-258	14
α-helix	261-274	14
α-helix	278-297	20
α-helix	299-301	3
α-helix	304-306	3
α-helix	310-319	10
α-helix	322-338	17
α-helix	345-357	13

Chain E: 23 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	5-7	3
α-helix	8-20	13
β-strand	26-30	5	7
α-helix	37-49	13
β-strand	54	1	8
β-strand	57	1	8
α-helix	63-70	8
β-strand	76-79	4	7
β-strand	88	1	9
α-helix	90-101	12
α-helix	103-104	2
β-strand	110-114	5	7
α-helix	117-119	3
β-strand	120	1	9
α-helix	122-133	12
α-helix	135-136	2
β-strand	139-145	7	7
α-helix	148-150	3
α-helix	153-157	5
β-strand	160-163	4	7
α-helix	166-168	3
α-helix	169-179	11
α-helix	184-193	10
α-helix	200-206	7
α-helix	209-226	18
α-helix	229-232	4
α-helix	233-236	4
α-helix	241-260	20
α-helix	271-280	10
α-helix	283-302	20
α-helix	308-323	16
α-helix	328-330	3

Chain F: 2 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	4-12	9
β-strand	16-19	4	4
α-helix	22-24	3

Chain H: 15 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	10
α-helix	7-18	12
α-helix	29-31	3
β-strand	32-38	7	11
β-strand	41-47	7	11
β-strand	51-58	8	11
β-strand	64	1	10
β-strand	66-71	6	11
α-helix	72-81	10
α-helix	83	1
β-strand	87-93	7	10
β-strand	96-101	6	10
β-strand	104-109	6	10
β-strand	111	1	11
α-helix	113-115	3
α-helix	119-121	3
β-strand	124-130	7	11
α-helix	132-142	11
α-helix	153-156	4
β-strand	158-172	15	12
β-strand	176-195	20	12
α-helix	197-206	10
α-helix	213	1
β-strand	214-219	6	11
β-strand	222-227	6	11
β-strand	230-235	6	11
α-helix	241-243	3
α-helix	245-247	3
β-strand	254-259	6	12
α-helix	260-272	13
β-strand	280-286	7	13
β-strand	289-295	7	13
β-strand	301-306	6	13
β-strand	309	1	12
β-strand	315-320	6	13
α-helix	321-330	10
β-strand	335-340	6	12
β-strand	347-351	5	12
β-strand	357-361	5	12
α-helix	362-364	3

Chain I: 13 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	14
α-helix	7-18	12
α-helix	28-31	4
β-strand	32-38	7	15
β-strand	41-47	7	15
β-strand	51-58	8	15
β-strand	64	1	14
β-strand	66-71	6	15
α-helix	72-81	10
α-helix	83	1
β-strand	87-93	7	14
β-strand	96-98	3	14
β-strand	101	1	16
β-strand	104	1	16
β-strand	105	1	13
β-strand	107-109	3	14
β-strand	111	1	15
α-helix	119-121	3
β-strand	124-130	7	15
α-helix	132-142	11
α-helix	153-156	4
β-strand	157-172	16	17
β-strand	176-196	21	17
α-helix	197-206	10
α-helix	213	1
β-strand	214-219	6	15
β-strand	222-227	6	15
β-strand	230-235	6	15
α-helix	244-247	4
α-helix	249	1
β-strand	254-259	6	17
α-helix	260-272	13
β-strand	280-286	7	10
β-strand	289-294	6	10
β-strand	302-306	5	10
β-strand	309	1	17
β-strand	315-320	6	10
α-helix	321-331	11
β-strand	335-340	6	17
β-strand	347-351	5	17
β-strand	358-361	4	17
β-strand	364	1	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA polymerase III subunit delta	A	protein	343	Escherichia coli K-12	P28630 (AlphaFold model)
DNA polymerase III subunit tau	B, C, D	protein	431	Escherichia coli K-12	P06710 (AlphaFold model)
DNA polymerase III subunit delta'	E	protein	334	Escherichia coli K-12	P28631 (AlphaFold model)
DNA polymerase III subunit psi	F	protein	137	Escherichia coli K-12	P28632 (AlphaFold model)
Beta sliding clamp	H, I	protein	366	Escherichia coli K-12	P0A988

Sequence of entity 1 (A), FASTA

>8GIY_1 DNA polymerase III subunit delta (chains A)
MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAAQGFEEHHTFSIDPNTD
WNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKA
QENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLA
LAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEG
SEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQ
AVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHKPLADVFIDG

Sequence of entity 2 (B, C, D), FASTA

>8GIY_2 DNA polymerase III subunit tau (chains B, C, D)
MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAK
GLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRF
KVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDV
EQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAM
LGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPA
ALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFH
PRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQ
RVQGATKAKKE

Sequence of entity 3 (E), FASTA

>8GIY_3 DNA polymerase III subunit delta' (chains E)
MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGHKSCG
HCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGAKVVWVTDAALL
TDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCRLHYLAPPPEQYAVTWLSREV
TMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQ
APARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQL
MSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL

Sequence of entity 4 (F), FASTA

>8GIY_4 DNA polymerase III subunit psi (chains F)
MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVANDLPALTDPLVSDVLRA
LTVSPDQVLQLTPEKIAMLPQGSHCNSWRLGTDEPLSLEGAQVASPALTDLRANPTARAA
LWQQICTYEHDFFPRND

Sequence of entity 5 (H, I), FASTA

>8GIY_5 Beta sliding clamp (chains H, I)
MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSLTGTDLEMEMVARVALV
QPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSGRSRFSLSTLPAADFPNLD
DWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLFETEGEELRTVATDGHRLAVC
SMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQIGSNNIRAHVGDFIFTSKLVDGR
FPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNEKFRGVRLYVSENQLKITANNPEQE
EAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCENVRMMLTDSVSSVQIEDAASQSAAYV
VMPMRL

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	4
MG	Magnesium ion	Mg	3
AGS	Phosphothiophosphoric acid-adenylate ester	C10 H16 N5 O12 P3 S	3

Primary citation

Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli. Xu, Z.Q., Jergic, S., Lo, A.T.Y. et al. Nat Commun (2024) 15:8372-8372. DOI 10.1038/s41467-024-52623-9 · PubMed

Other PDB entries of the same protein (UniProt P28630 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1JQL 2.5 Å, Mechanism of Processivity Clamp Opening by the Delta Subunit Wrench of the Clamp Loader…
9OYJ 2.53 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
9OYH 2.54 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
9OYI 2.54 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
8GJ2 2.6 Å, E. coli clamp loader with closed clamp on primed template DNA
9OYK 2.6 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
9OYM 2.6 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
1JR3 2.7 Å, Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA…
8GIZ 2.7 Å, E. coli clamp loader with open clamp
9OYN 2.7 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
9OYC 2.71 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 1-nt gapped…
9OYE 2.72 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 1-nt gapped…

Browse structure collections

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