8GJ3: E. coli clamp loader on primed template DNA

E. coli clamp loader on primed template DNA. Determined by electron microscopy at 2.8 Å resolution. Released 27 Mar 2024.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Escherichia coli K-12, Escherichia coli
Chains: 8
Atoms: 14,894
Mol. weight: 319.35 kDa
Ligands: ALF, MG, ADP, ZN
Released: 27 Mar 2024

Explore 8GJ3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8GJ3 contains 114 α-helices and 36 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 20 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	3-4	2	1
α-helix	6-15	10
β-strand	20-25	6	1
α-helix	28-45	18
β-strand	49-52	4	1
α-helix	61-70	10
β-strand	78-83	6	1
α-helix	91-102	12
β-strand	108-114	7	1
α-helix	116-118	3
α-helix	119-122	4
α-helix	125-129	5
β-strand	134-139	6	1
α-helix	140-142	3
α-helix	147-157	11
β-strand	161-162	2	2
α-helix	164-173	10
α-helix	178-191	14
β-strand	196-197	2	2
α-helix	199-204	6
α-helix	214-223	10
α-helix	226-239	14
α-helix	243-263	21
α-helix	269-275	7
α-helix	283-292	10
α-helix	295-310	16
α-helix	311-315	5
α-helix	320-331	12

Chain B: 24 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-9	4
α-helix	22-34	13
β-strand	40-44	5	3
α-helix	51-62	12
α-helix	77-84	8
β-strand	90-94	5	3
α-helix	101-109	9
α-helix	114-115	2
β-strand	121-126	6	3
α-helix	128-130	3
α-helix	133-144	12
β-strand	150-155	6	3
α-helix	159-161	3
α-helix	164-167	4
β-strand	171-174	4	3
α-helix	175-177	3
α-helix	181-193	13
α-helix	200-210	11
α-helix	214-227	14
α-helix	234-243	10
α-helix	248-258	11
α-helix	261-274	14
α-helix	278-297	20
α-helix	299-301	3
α-helix	304-309	6
α-helix	310-319	10
α-helix	322-338	17
α-helix	339-341	3
α-helix	345-357	13
β-strand	360-361	2	4

Chain C: 25 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	6-9	4
α-helix	15-17	3
α-helix	22-34	13
β-strand	40-44	5	5
α-helix	51-62	12
α-helix	77-84	8
β-strand	90-94	5	5
α-helix	101-108	8
α-helix	114-115	2
β-strand	121-126	6	5
α-helix	128-130	3
α-helix	133-144	12
β-strand	150-156	7	5
α-helix	159-161	3
α-helix	164-168	5
β-strand	171-174	4	5
α-helix	177-179	3
α-helix	180-194	15
β-strand	197-198	2	6
α-helix	200-210	11
α-helix	214-227	14
β-strand	231-232	2	6
α-helix	234-241	8
α-helix	246-257	12
α-helix	261-274	14
α-helix	278-297	20
α-helix	299-301	3
α-helix	310-319	10
α-helix	322-338	17
α-helix	339-341	3
α-helix	345-358	14
α-helix	364-368	5

Chain D: 23 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	6-9	4
α-helix	15-17	3
α-helix	22-34	13
β-strand	40-44	5	7
α-helix	51-62	12
α-helix	77-84	8
β-strand	91-94	4	7
α-helix	101-110	10
α-helix	114-115	2
β-strand	121-126	6	7
α-helix	128-130	3
α-helix	133-144	12
β-strand	150-156	7	7
α-helix	159-161	3
α-helix	164-168	5
β-strand	171-174	4	7
α-helix	175-179	5
α-helix	180-194	15
α-helix	200-210	11
α-helix	214-227	14
α-helix	234-240	7
α-helix	248-258	11
α-helix	261-274	14
α-helix	278-297	20
α-helix	299-301	3
α-helix	310-319	10
α-helix	322-338	17
α-helix	345-357	13

Chain E: 21 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	5-7	3
α-helix	8-19	12
β-strand	26-30	5	8
α-helix	37-49	13
β-strand	54	1	9
β-strand	57	1	9
α-helix	63-70	8
β-strand	76-79	4	8
β-strand	88	1	10
α-helix	90-101	12
α-helix	103-104	2
β-strand	110-114	5	8
α-helix	117-119	3
β-strand	120	1	10
α-helix	122-133	12
β-strand	139-145	7	8
α-helix	148-150	3
α-helix	153-156	4
β-strand	160-163	4	8
α-helix	164-168	5
α-helix	169-179	11
α-helix	184-193	10
α-helix	200-206	7
α-helix	209-226	18
α-helix	229-232	4
α-helix	233-236	4
α-helix	241-260	20
α-helix	271-280	10
α-helix	283-302	20
α-helix	308-323	16

Chain F: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
α-helix	4-12	9
β-strand	17-18	2	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA polymerase III subunit delta	A	protein	343	Escherichia coli K-12	P28630 (AlphaFold model)
DNA polymerase III subunit tau	B, C, D	protein	643	Escherichia coli K-12	P06710 (AlphaFold model)
DNA polymerase III subunit delta'	E	protein	334	Escherichia coli K-12	P28631 (AlphaFold model)
DNA polymerase III subunit psi	F	protein	137	Escherichia coli K-12	P28632 (AlphaFold model)
Primer	X	DNA	16	Escherichia coli
Template	Y	DNA	26	Escherichia coli

Sequence of entity 1 (A), FASTA

>8GJ3_1 DNA polymerase III subunit delta (chains A)
MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAAQGFEEHHTFSIDPNTD
WNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKA
QENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLA
LAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEG
SEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQ
AVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHKPLADVFIDG

Sequence of entity 2 (B, C, D), FASTA

>8GJ3_2 DNA polymerase III subunit tau (chains B, C, D)
MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAK
GLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRF
KVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDV
EQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAM
LGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPA
ALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFH
PRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQ
RVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKA
TTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAAQVSQLSLPKLVEQVA
LNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPL
EWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI

Sequence of entity 3 (E), FASTA

>8GJ3_3 DNA polymerase III subunit delta' (chains E)
MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGHKSCG
HCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGAKVVWVTDAALL
TDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCRLHYLAPPPEQYAVTWLSREV
TMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQ
APARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQL
MSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL

Sequence of entity 4 (F), FASTA

>8GJ3_4 DNA polymerase III subunit psi (chains F)
MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVANDLPALTDPLVSDVLRA
LTVSPDQVLQLTPEKIAMLPQGSHCNSWRLGTDEPLSLEGAQVASPALTDLRANPTARAA
LWQQICTYEHDFFPRND

Sequence of entity 5 (X), FASTA

>8GJ3_5 Primer (chains X)
TTAGTTACAACATACT

Sequence of entity 6 (Y), FASTA

>8GJ3_6 Template (chains Y)
TTTTTTTTTTTAGTATGTTGTAACTA

Ligands and cofactors

ID	Name	Formula	Copies
ALF	Tetrafluoroaluminate ion	Al F4	3
MG	Magnesium ion	Mg	3
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	3
ZN	Zinc ion	Zn	4

Primary citation

Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli. Xu, Z.Q., Jergic, S., Lo, A.T.Y. et al. Nat Commun (2024) 15:8372-8372. DOI 10.1038/s41467-024-52623-9 · PubMed

Other PDB entries of the same protein (UniProt P28630 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1JQL 2.5 Å, Mechanism of Processivity Clamp Opening by the Delta Subunit Wrench of the Clamp Loader…
9OYJ 2.53 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
9OYH 2.54 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
9OYI 2.54 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
8GJ2 2.6 Å, E. coli clamp loader with closed clamp on primed template DNA
9OYK 2.6 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
9OYM 2.6 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
1JR3 2.7 Å, Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA…
8GIZ 2.7 Å, E. coli clamp loader with open clamp
9OYN 2.7 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
9OYC 2.71 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 1-nt gapped…
9OYE 2.72 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 1-nt gapped…

Browse structure collections

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