8HCQ: Endothelin1-bound ETAR-Gq complex

Cryo-EM structure of endothelin1-bound ETAR-Gq complex. Determined by electron microscopy at 3.01 Å resolution. Released 22 Mar 2023.

Method: Electron microscopy
Resolution: 3.01 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 8,991
Mol. weight: 179.83 kDa
Released: 22 Mar 2023

Explore 8HCQ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8HCQ contains 31 α-helices and 69 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	9-26	18
β-strand	35-40	6	1
β-strand	183	1	1
β-strand	186-189	4	1
β-strand	192-198	7	1
α-helix	210-213	4
β-strand	218-222	5	1
β-strand	224	1	2
α-helix	231-242	12
β-strand	251-256	6	1
β-strand	257	1	2
α-helix	259-267	9
α-helix	291-292	2
α-helix	297-315	19
β-strand	324-328	5	1
α-helix	336-356	21

Chain B: 5 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	9-12	4
α-helix	14-30	17
α-helix	36-39	4
α-helix	43-44	2
β-strand	52-56	5	3
β-strand	63-68	6	4
β-strand	74-79	6	4
β-strand	83-88	6	4
β-strand	93-99	7	4
β-strand	105-110	6	5
β-strand	116-121	6	5
β-strand	126-130	5	5
β-strand	139-144	6	5
β-strand	151-156	6	6
β-strand	161-166	6	6
β-strand	171-175	5	6
β-strand	181-185	5	6
β-strand	192-197	6	7
β-strand	203-208	6	7
β-strand	213-217	5	7
β-strand	226-227	2	7
β-strand	234-239	6	8
β-strand	245-250	6	8
β-strand	255-259	5	8
β-strand	264-269	6	8
α-helix	277-278	2
β-strand	281-283	3	9
β-strand	289-292	4	9
β-strand	298	1	10
β-strand	300-303	4	9
β-strand	309-311	3	9
β-strand	314	1	10
β-strand	320-325	6	3
β-strand	332-336	5	3
β-strand	341-344	4	3

Chain E: 3 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	2-5	4	11
β-strand	9-11	3	12
β-strand	17-24	8	11
β-strand	32-38	7	13
β-strand	44-50	7	13
α-helix	52-54	3
β-strand	57-59	3	13
β-strand	68-72	5	11
β-strand	77-82	6	11
β-strand	92-98	7	13
β-strand	109-110	2	13
β-strand	114-115	2	13
β-strand	116-118	3	12
β-strand	140-141	2	14
β-strand	146-148	3	15
β-strand	155-161	7	14
β-strand	174-179	6	15
β-strand	185-189	5	15
β-strand	191	1	16
β-strand	194	1	16
β-strand	203-207	5	14
β-strand	211-216	6	14
α-helix	221-223	3
β-strand	226-231	6	15
β-strand	239	1	15
α-helix	240-242	3
β-strand	243-246	4	15

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-24	18
α-helix	30-43	14
α-helix	53-55	3

Chain L: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-17	9
α-helix	19-20	2

Chain R: 11 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	77-108	32
β-strand	113	1	17
α-helix	114-142	29
α-helix	152-158	7
α-helix	161-188	28
β-strand	197	1	17
α-helix	200-221	22
β-strand	226	1	18
β-strand	230-231	2	19
β-strand	234-235	2	19
β-strand	239	1	18
α-helix	246-252	7
α-helix	255-260	6
α-helix	261-265	5
α-helix	266-282	17
α-helix	298-329	32
α-helix	340-372	33

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(q) subunit alpha-1	A	protein	246	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	377	Homo sapiens	P62873 (AlphaFold model)
scFv16	E	protein	285	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Endothelin-1	L	protein	21	Homo sapiens	P05305 (AlphaFold model)
Endothelin-1 receptor,Oplophorus-luciferin 2-monooxygenase catalytic subunit chimera	R	protein	622	Homo sapiens	Q9GV45 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8HCQ_1 Guanine nucleotide-binding protein G(q) subunit alpha-1 (chains A)
MGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNL
REYNLV

Sequence of entity 2 (B), FASTA

>8HCQ_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSG
GGGSSGVSGWRLFKKIS

Sequence of entity 3 (E), FASTA

>8HCQ_3 scFv16 (chains E)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFAVQLVESGGGLVQPGGSRKLSCS
ASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQM
TSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSAGGGGSGGGGSGGGGSADIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLEL

Sequence of entity 4 (G), FASTA

>8HCQ_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (L), FASTA

>8HCQ_5 Endothelin-1 (chains L)
CSCSSLMDKECVYFCHLDIIW

Sequence of entity 6 (R), FASTA

>8HCQ_6 Endothelin-1 receptor,Oplophorus-luciferin 2-monooxygenase catalytic subunit chimera (chains R)
MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSDYKDDDDVDMGQPGNGSAFLLAPNGSHAPD
HDVTQQRDEENLYFQGASDNPERYSTNLSNHVDDFTTFRGTELSFLVTTHQPTNLVLPSN
GSMHNYCPQQTKITSAFKYINTVISCTIFIVGMVGNATLLRIIYQNKCMRNGPNALIASL
ALGDLIYVVIDLPINVFKLLAGRWPFDHNDFGVFLCKLFPFLQKSSVGITVLNLCALSVD
RYRAVASWSRVQGIGIPLVTAIEIVSIWILSFILAIPEAIGFVMVPFEYRGEQHKTCMLN
ATSKFMEFYQDVKDWWLFGFYFCMPLVCTAIFYTLMTCEMLNRRNGSLRIALSEHLKQRR
EVAKTVFCLVVIFALCWFPLHLSRILKKTVYNEMDKNRCELLSFLLLMDYIGINLATMNS
CINPIALYFVSKKFKNCFQSCLCCCCYQSKSLMTSVPMNGTSIQVFTLEDFVGDWEQTAA
YNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEE
VFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGN
KIIDERLITPDGSMLFRVTINS

Primary citation

Structural basis of peptide recognition and activation of endothelin receptors. Ji, Y., Duan, J., Yuan, Q. et al. Nat Commun (2023) 14:1268-1268. DOI 10.1038/s41467-023-36998-9 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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