8HCX: Endothelin1-bound ETBR-Gq complex

Cryo-EM structure of Endothelin1-bound ETBR-Gq complex. Determined by electron microscopy at 3.5 Å resolution. Released 22 Mar 2023.

Method: Electron microscopy
Resolution: 3.5 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 8,933
Mol. weight: 177.75 kDa
Released: 22 Mar 2023

Explore 8HCX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8HCX contains 36 α-helices and 58 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	9-28	20
β-strand	34-40	7	1
β-strand	183-189	7	1
β-strand	192-198	7	1
α-helix	209-214	6
β-strand	218-224	7	1
α-helix	231-242	12
β-strand	251-257	7	1
α-helix	259-267	9
α-helix	273-275	3
α-helix	278-280	3
α-helix	290-292	3
α-helix	297-316	20
β-strand	325-328	4	1
α-helix	336-356	21

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	10-30	21
α-helix	35-39	5
α-helix	43-44	2
β-strand	54-57	4	2
β-strand	63-68	6	3
β-strand	74-79	6	3
β-strand	83-88	6	3
β-strand	94-99	6	3
β-strand	107-110	4	4
β-strand	116-120	5	4
β-strand	126-130	5	4
β-strand	139-144	6	4
β-strand	151-156	6	5
β-strand	161-166	6	5
β-strand	171-175	5	5
β-strand	181-185	5	5
β-strand	194-197	4	6
β-strand	203-207	5	6
β-strand	213-217	5	6
β-strand	222-227	6	6
β-strand	234-239	6	7
β-strand	245-250	6	7
β-strand	254-259	6	7
β-strand	264-270	7	7
β-strand	278-283	6	8
β-strand	289-294	6	8
β-strand	299-303	5	8
β-strand	309-313	5	8
β-strand	320-325	6	2
β-strand	332-336	5	2
β-strand	340-344	5	2

Chain C: 12 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	98-128	31
α-helix	135-163	29
α-helix	170-174	5
α-helix	176-203	28
α-helix	216-237	22
β-strand	240-245	6	9
β-strand	252-257	6	9
α-helix	264-271	8
α-helix	273-278	6
α-helix	279-283	5
α-helix	284-301	18
α-helix	316-349	34
α-helix	357-389	33
α-helix	391-394	4

Chain D: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-17	9
α-helix	19-20	2

Chain E: 6 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	10
β-strand	9-11	3	11
β-strand	17-24	8	10
α-helix	28-30	3
β-strand	32-38	7	12
β-strand	44-50	7	12
α-helix	52-54	3
β-strand	57-59	3	12
α-helix	61-63	3
β-strand	68-72	5	10
β-strand	77-82	6	10
α-helix	87-89	3
β-strand	91-93	3	11
β-strand	94-98	5	12
β-strand	109-110	2	12
β-strand	114-118	5	11
β-strand	140-142	3	13
β-strand	146-148	3	14
β-strand	156-161	6	13
β-strand	174-179	6	14
β-strand	186-189	4	14
β-strand	203-207	5	13
β-strand	211-216	6	13
α-helix	221-223	3
β-strand	226-231	6	14
β-strand	239	1	14
α-helix	240-242	3
β-strand	243-246	4	14

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(q) subunit alpha-1	A	protein	246	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	377	Homo sapiens	P62873 (AlphaFold model)
Endothelin receptor type B,Oplophorus-luciferin 2-monooxygenase catalytic subunit chimera	C	protein	603	Homo sapiens	Q9GV45 (AlphaFold model)
Endothelin-1	D	protein	21	Homo sapiens	P05305 (AlphaFold model)
scFv16	E	protein	285	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8HCX_1 Guanine nucleotide-binding protein G(q) subunit alpha-1 (chains A)
MGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNL
REYNLV

Sequence of entity 2 (B), FASTA

>8HCX_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSG
GGGSSGVSGWRLFKKIS

Sequence of entity 3 (C), FASTA

>8HCX_3 Endothelin receptor type B,Oplophorus-luciferin 2-monooxygenase catalytic subunit chimera (chains C)
MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSDYKDDDDVDHHHHHHHHEERGFPPDRATPL
LQTAEIMTPPTKTLWPKGSNASLARSLAPAEVPKGDRTAGSPPRTISPPPCQGPIEIKET
FKYINTVVSCLVFVLGIIGNSTLLRIIYKNKCMRNGPNILIASLALGDLLHIVIDIPINV
YKLLAEDWPFGAEMCKLVPFIQKASVGITVLSLCALSIDRYRAVASWSRIKGIGVPKWTA
VEIVLIWVVSVVLAVPEAIGFDIITMDYKGSYLRICLLHPVQKTAFMQFYKTAKDWWLFS
FYFCLPLAITAFFYTLMTCEMLRKKSGMQIALNDHLKQRREVAKTVFCLVLVFALCWLPL
HLSRILKLTLYNQNDPNRCELLSFLLVLDYIGINMASLNSCINPIALYLVSKRFKNCFKS
CLCCWCQSFEEKQSLEEKQSCLKFKVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNL
AVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPY
GTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVT
INS

Sequence of entity 4 (D), FASTA

>8HCX_4 Endothelin-1 (chains D)
CSCSSLMDKECVYFCHLDIIW

Sequence of entity 5 (E), FASTA

>8HCX_5 scFv16 (chains E)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFAVQLVESGGGLVQPGGSRKLSCS
ASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQM
TSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSAGGGGSGGGGSGGGGSADIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLEL

Sequence of entity 6 (G), FASTA

>8HCX_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

Structural basis of peptide recognition and activation of endothelin receptors. Ji, Y., Duan, J., Yuan, Q. et al. Nat Commun (2023) 14:1268-1268. DOI 10.1038/s41467-023-36998-9 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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