8HXA: MPXV M2

Cryo-EM structure of MPXV M2 in complex with human B7.1. Determined by electron microscopy at 3.04 Å resolution. Released 30 Aug 2023.

Method: Electron microscopy
Resolution: 3.04 Å
Organisms: Monkeypox virus, Homo sapiens
Chains: 12
Atoms: 14,790
Mol. weight: 277.08 kDa
Released: 30 Aug 2023

Explore 8HXA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8HXA contains 41 α-helices and 152 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	22	1	1
α-helix	24-27	4
β-strand	33-44	12	2
β-strand	57-62	6	3
β-strand	67-73	7	3
β-strand	76-82	7	3
β-strand	89-96	8	2
α-helix	98-100	3
β-strand	101-108	8	2
β-strand	119-125	7	3
β-strand	130	1	3
β-strand	131	1	4
β-strand	132-133	2	3
β-strand	137-140	4	3
β-strand	148-155	8	3
β-strand	158-165	8	2
α-helix	176-177	2
β-strand	181-184	4	2
α-helix	188-190	3
β-strand	192-197	6	2
β-strand	215-216	2	2
α-helix	217	1
β-strand	218	1	3

Chains B, C, D and F: 5 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	22	1	5
α-helix	24-27	4
β-strand	33-44	12	6
β-strand	57-62	6	7
β-strand	67-73	7	7
β-strand	76-82	7	7
β-strand	89-96	8	6
α-helix	98-100	3
β-strand	101-108	8	6
β-strand	119-125	7	7
β-strand	130-133	4	7
β-strand	137-140	4	7
β-strand	148-155	8	7
β-strand	158-165	8	6
α-helix	176-177	2
β-strand	181-184	4	6
α-helix	188-190	3
β-strand	192-197	6	6
β-strand	215-216	2	6
α-helix	217	1
β-strand	218	1	7

Chain E: 4 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	22	1	14
α-helix	24-27	4
β-strand	33-44	12	15
β-strand	57-62	6	16
β-strand	67-73	7	16
β-strand	76-82	7	16
β-strand	89-96	8	15
α-helix	98-100	3
β-strand	101-108	8	15
β-strand	119-125	7	16
β-strand	130-133	4	16
β-strand	137-140	4	16
β-strand	148-155	8	16
β-strand	158-165	8	15
α-helix	176-177	2
β-strand	181-184	4	15
α-helix	188-190	3
β-strand	192-197	6	15
β-strand	215-216	2	15
β-strand	218	1	16

Chains G, H, I, J, K and L: 2 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	36-41	6	2
β-strand	46-48	3	19
α-helix	56-59	4
β-strand	62-68	7	2
β-strand	71-77	7	2
β-strand	80-83	4	2
β-strand	91-94	4	19
β-strand	100-103	4	19
α-helix	108-110	3
β-strand	112-121	10	2
β-strand	126-139	14	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
NFkB inhibitor	A, B, C, D, E, F	protein	203	Monkeypox virus	A0A7H0DN11
T-lymphocyte activation antigen CD80	G, H, I, J, K, L	protein	200	Homo sapiens	P33681 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F), FASTA

>8HXA_1 NFkB inhibitor (chains A, B, C, D, E, F)
VEYKNTICPPRQDYRYWYFVAELTIGVNYDINSTIIGECHMSESYIDRNANIVLTGYGLK
INMTIMDTDQRFVAAAEGVGKDNKLSVLLFTTQRLDKVHHNISVTITCMEMNCGTTKYNS
DLPESIHKSSSCDITINGSCVTCVNLETDPTKINPHYLHPKNKYLYHNSEYSMRGSYGVT
FIDELNQCLLDIKELSYDICYRE

Sequence of entity 2 (G, H, I, J, K, L), FASTA

>8HXA_2 T-lymphocyte activation antigen CD80 (chains G, H, I, J, K, L)
VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFD
ITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPT
SNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSF
MCLIKYGHLRVNQTFNWNTT

Primary citation

Structural and functional insights into the modulation of T cell costimulation by monkeypox virus protein M2. Yang, S., Wang, Y., Yu, F. et al. Nat Commun (2023) 14:5186-5186. DOI 10.1038/s41467-023-40748-2 · PubMed

Other PDB entries of the same protein (UniProt A0A7H0DN11), best resolution first:

8HXB 2.7 Å, Cryo-EM structure of MPXV M2 hexamer in complex with human B7.2
8HXC 3.12 Å, Cryo-EM structure of MPXV M2 heptamer in complex with human B7.2

Browse structure collections

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