8IOC: PDB entry 8IOC
Cryo-EM structure of the gamma-MSH-bound human melanocortin receptor 3 (MC3R)-Gs complex. Determined by electron microscopy at 2.86 Å resolution. Released 20 Sept 2023.
- Method
- Electron microscopy
- Resolution
- 2.86 Å
- Organisms
- Homo sapiens, Influenza A virus (strain A/Victoria/3/1975 H3N2), Bos taurus
- Chains
- 6
- Atoms
- 8,223
- Mol. weight
- 165.23 kDa
- Ligands
- CA
- Released
- 20 Sept 2023
Explore 8IOC in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8IOC contains 27 α-helices and 47 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 9 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-38 | 25 | |
| β-strand | 40-46 | 7 | 1 |
| α-helix | 53-59 | 7 | |
| β-strand | 208-214 | 7 | 1 |
| β-strand | 217-223 | 7 | 1 |
| α-helix | 235-238 | 4 | |
| β-strand | 243-249 | 7 | 1 |
| α-helix | 266-278 | 13 | |
| β-strand | 286-292 | 7 | 1 |
| α-helix | 294-303 | 10 | |
| α-helix | 308-310 | 3 | |
| α-helix | 326-327 | 2 | |
| α-helix | 332-352 | 21 | |
| β-strand | 359-362 | 4 | 1 |
| α-helix | 371-390 | 20 | |
Chain B: 3 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-24 | 21 | |
| α-helix | 32-34 | 3 | |
| α-helix | 38-39 | 2 | |
| β-strand | 46-51 | 6 | 2 |
| β-strand | 58-62 | 5 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 88-94 | 7 | 3 |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 120-125 | 6 | 4 |
| β-strand | 134-140 | 7 | 4 |
| β-strand | 146-153 | 8 | 5 |
| β-strand | 156-161 | 6 | 5 |
| β-strand | 165-170 | 6 | 5 |
| β-strand | 176-181 | 6 | 5 |
| β-strand | 187-192 | 6 | 6 |
| β-strand | 198-203 | 6 | 6 |
| β-strand | 206-212 | 7 | 6 |
| β-strand | 218-222 | 5 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 250-254 | 5 | 7 |
| β-strand | 260-264 | 5 | 7 |
| β-strand | 273-278 | 6 | 8 |
| β-strand | 285-289 | 5 | 8 |
| β-strand | 293-297 | 5 | 8 |
| β-strand | 307-309 | 3 | 8 |
| β-strand | 315 | 1 | 9 |
| β-strand | 319-320 | 2 | 2 |
| β-strand | 327-330 | 4 | 2 |
| β-strand | 331 | 1 | 9 |
| β-strand | 336-340 | 5 | 2 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-22 | 14 | |
| α-helix | 30-33 | 4 | |
| α-helix | 40-44 | 5 | |
| α-helix | 53-55 | 3 | |
Chain N: 1 helix, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-6 | 4 | 10 |
| β-strand | 10-12 | 3 | 11 |
| β-strand | 17-25 | 9 | 10 |
| β-strand | 34-39 | 6 | 11 |
| β-strand | 45-51 | 7 | 11 |
| β-strand | 58-60 | 3 | 11 |
| β-strand | 69-73 | 5 | 10 |
| β-strand | 78-84 | 7 | 10 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 11 |
| β-strand | 118 | 1 | 11 |
| β-strand | 122-126 | 5 | 11 |
Chain R: 10 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 42-64 | 23 | |
| α-helix | 72-102 | 31 | |
| α-helix | 110-147 | 38 | |
| α-helix | 152-154 | 3 | |
| α-helix | 158-181 | 24 | |
| α-helix | 186-222 | 37 | |
| α-helix | 236-267 | 32 | |
| α-helix | 272-278 | 7 | |
| α-helix | 281-298 | 18 | |
| α-helix | 304-312 | 9 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s)… | A | protein | 361 | Homo sapiens | P63092 (AlphaFold model), P63096 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT | B | protein | 371 | Homo sapiens | P62873 (AlphaFold model) |
| HA signal peptide,Melanocortin receptor 3,LgBiT subunit | R | protein | 511 | Influenza A virus (strain A/Victoria/3/1975 H3N2), Homo sapiens | P03435, P41968 |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Bos taurus | P63212 |
| gamma-melanocyte-stimulating hormone | L | protein | 11 | synthetic construct | P01189 |
| Nanobody-35 | N | protein | 160 | Homo sapiens | |
Sequence of entity 1 (A), FASTA
>8IOC_1 Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L
Sequence of entity 2 (B), FASTA
>8IOC_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSG
VSGWRLFKKIS
Sequence of entity 3 (R), FASTA
>8IOC_3 HA signal peptide,Melanocortin receptor 3,LgBiT subunit (chains R)
MKTIIALSYIFCLVFANASCCLPSVQPTLPNGSEHLQAPFFSNQSSSAFCEQVFIKPEVF
LSLGIVSLLENILVILAVVRNGNLHSPMYFFLCSLAVADMLVSVSNALETIMIAIVHSDY
LTFEDQFIQHMDNIFDSMICISLVASICNLLAIAVDRYVTIFYALRYHSIMTVRKALTLI
VAIWVCCGVCGVVFIVYSESKMVIVCLITMFFAMMLLMGTLYVHMFLFARLHVKRIAALP
PADGVAPQQHSCMKGAVTITILLGVFIFCWAPFFLHLVLIITCPTNPYCICYTAHFNTYL
VLIMCNSVIDPLIYAFRSLELRNTFREILCGCNGMNLGGSSGGGGSGGGGSSGVFTLEDF
VGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLS
ADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKIT
VTGTLWNGNKIIDERLITPDGSMLFRVTINS
Sequence of entity 4 (G), FASTA
>8IOC_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 5 (L), FASTA
>8IOC_5 gamma-melanocyte-stimulating hormone (chains L)
YVMGHFRWDRF
Sequence of entity 6 (N), FASTA
>8IOC_6 Nanobody-35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CA | Calcium ion | Ca | 1 |
Primary citation
Structural insights into ligand recognition and subtype selectivity of the human melanocortin-3 and melanocortin-5 receptors. Feng, W., Zhou, Q., Chen, X. et al. Cell Discov (2023) 9:81. DOI 10.1107/S0907444909042073 · PubMed
Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
- 6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
- 7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)
Browse structure collections
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