8JLQ: Fenoldopam-bound hTAAR1-Gs protein complex

Fenoldopam-bound hTAAR1-Gs protein complex. Determined by electron microscopy at 2.84 Å resolution. Released 15 Nov 2023.

Method: Electron microscopy
Resolution: 2.84 Å
Organisms: Homo sapiens, Escherichia coli, Mus musculus
Chains: 5
Atoms: 9,033
Mol. weight: 171.54 kDa
Ligands: G3C
Released: 15 Nov 2023

Explore 8JLQ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8JLQ contains 31 α-helices and 62 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	14-38	25
β-strand	40-47	8	1
α-helix	53-61	9
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	234-237	4
β-strand	243-249	7	1
α-helix	265-277	13
α-helix	280-282	3
β-strand	286-292	7	1
α-helix	294-297	4
α-helix	298-302	5
α-helix	332-350	19
β-strand	359-363	5	1
α-helix	370-390	21

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-25	21
α-helix	30-33	4
β-strand	48-52	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	176-181	6	5
α-helix	186	1
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	218-223	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	295-298	4	8
β-strand	303-307	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain R: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	23-50	28
α-helix	52-54	3
α-helix	57-86	30
α-helix	93-123	31
α-helix	128-130	3
α-helix	131-134	4
α-helix	137-159	23
α-helix	169-179	11
α-helix	188-194	7
α-helix	195-200	6
α-helix	201-231	31
α-helix	249-276	28
α-helix	283-304	22
α-helix	309-318	10

Chain S: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	11-12	2	10
β-strand	16-25	10	9
β-strand	33-39	7	11
β-strand	45-51	7	11
β-strand	58-60	3	11
β-strand	68-73	6	9
β-strand	78-86	9	9
β-strand	93-94	2	11
β-strand	97-99	3	11
β-strand	111	1	11
β-strand	115-116	2	11
β-strand	118-119	2	10
α-helix	138-139	2
β-strand	140-142	3	12
β-strand	146-148	3	13
β-strand	155-161	7	12
β-strand	166	1	14
β-strand	172	1	14
β-strand	174-176	3	15
β-strand	179	1	13
α-helix	180	1
β-strand	189-190	2	15
β-strand	194-195	2	15
β-strand	203-207	5	12
β-strand	211-216	6	12
α-helix	221-223	3
β-strand	225-226	2	13
β-strand	229-231	3	15
β-strand	239	1	15
β-strand	244-246	3	13

Chain Y: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	7-23	17
α-helix	30-43	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
mGs	A	protein	371	Homo sapiens	P63092 (AlphaFold model), P63096 (AlphaFold model), Q5JWF2 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	358	Homo sapiens	P62873 (AlphaFold model)
Soluble cytochrome b562,Trace amine-associated receptor 1	R	protein	470	Escherichia coli, Homo sapiens	P0ABE7, Q96RJ0
ScFv16	S	protein	251	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	Y	protein	71	Homo sapiens	P59768

Sequence of entity 1 (A), FASTA

>8JLQ_1 mGs (chains A)
MGCTLRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQT
NRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPED
ATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENIRRVFNDCRDII
QRMHLRQYELL

Sequence of entity 2 (B), FASTA

>8JLQ_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (R), FASTA

>8JLQ_3 Soluble cytochrome b562,Trace amine-associated receptor 1 (chains R)
DYKDDDDKHHHHHHHHHHENLYFQGADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRA
AALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLK
TTRNAYIQKYLMMPFCHNIINISCVKNNWSNDVRASLYSLMVLIILTTLVGNLIVIVSIS
HFKQLHTPTNWLIHSMATVDFLLGCLVMPYSMVRSAEHCWYFGEVFCKIHTSTDIMLSSA
SIFHLSFISIDRYYAVCDPLRYKAKMNILVICVMIFISWSVPAVFAFGMIFLELNFKGAE
EIYYKHVHCRGGCSVFFSKISGVLTFMTSFYIPGSIMLCVYYRIYLIAKEQARLISDANQ
KLQIGLEMKNGISQSKERKAVKTLGIVMGVFLICWCPFFICTVMDPFLHYIIPPTLNDVL
IWFGYLNSTFNPMVYAFFYPWFRKALKMMLFGKIFQKDSSRCKLFLELSS

Sequence of entity 4 (S), FASTA

>8JLQ_4 ScFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGSSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAA

Sequence of entity 5 (Y), FASTA

>8JLQ_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains Y)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
G3C	(1R)-6-chloranyl-1-(4-hydroxyphenyl)-2,3,4,5-tetrahydro-1H-3-benzazepine-7,8-di…	C16 H16 Cl N O3	1

Primary citation

Ligand recognition and G-protein coupling of trace amine receptor TAAR1. Xu, Z., Guo, L., Yu, J. et al. Nature (2023) 624:672-681. DOI 10.1038/s41586-023-06804-z · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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