8JSO: AMPH-bound hTAAR1-Gs protein complex

AMPH-bound hTAAR1-Gs protein complex. Determined by electron microscopy at 3.4 Å resolution. Released 15 Nov 2023.

Method: Electron microscopy
Resolution: 3.4 Å
Organisms: Homo sapiens, Escherichia coli, Mus musculus
Chains: 5
Atoms: 9,045
Mol. weight: 169.86 kDa
Ligands: 1WE
Released: 15 Nov 2023

Explore 8JSO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8JSO contains 29 α-helices and 68 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-37	4	1
α-helix	46-53	8
β-strand	184	1	2
β-strand	187-191	5	1
β-strand	194-199	6	1
β-strand	201	1	2
β-strand	220-226	7	1
α-helix	233-243	11
β-strand	253-259	7	1
α-helix	261-270	10
α-helix	275-278	4
α-helix	280-282	3
α-helix	293-294	2
α-helix	299-317	19
β-strand	326	1	1
β-strand	330	1	1
α-helix	339-357	19

Chain B: 3 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	4-23	20
α-helix	30-34	5
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100	1	5
β-strand	104-105	2	6
β-strand	111-114	4	6
β-strand	116	1	5
β-strand	120-121	2	7
β-strand	122-125	4	6
β-strand	134-135	2	6
β-strand	139-140	2	7
β-strand	146-151	6	8
β-strand	157-161	5	8
β-strand	166-168	3	8
β-strand	178-180	3	8
α-helix	186-187	2
β-strand	190-192	3	9
β-strand	198-202	5	9
β-strand	207-212	6	9
β-strand	217-223	7	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	259-264	6	10
β-strand	273-278	6	11
β-strand	284-289	6	11
β-strand	294-298	5	11
β-strand	304-307	4	11
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain R: 13 helices, 0 β-strands

Element	Residues	Length
α-helix	20-50	31
α-helix	57-75	19
α-helix	77-85	9
α-helix	92-122	31
α-helix	128-130	3
α-helix	137-159	23
α-helix	169-177	9
α-helix	188-195	8
α-helix	196-200	5
α-helix	201-227	27
α-helix	248-276	29
α-helix	283-304	22
α-helix	309-318	10

Chain S: 2 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	12
β-strand	10-12	3	13
β-strand	17-25	9	12
α-helix	29-31	3
β-strand	33-39	7	14
β-strand	45-51	7	14
β-strand	58-60	3	14
β-strand	69-73	5	12
β-strand	78-84	7	12
β-strand	93-99	7	14
β-strand	111	1	14
β-strand	115-116	2	14
β-strand	117-119	3	13
β-strand	140-142	3	15
β-strand	146-148	3	16
β-strand	155-161	7	15
β-strand	175-176	2	17
β-strand	179	1	16
β-strand	181	1	18
β-strand	183	1	18
β-strand	189-190	2	17
β-strand	194-195	2	17
β-strand	203-207	5	15
β-strand	211-216	6	15
α-helix	221-223	3
β-strand	226-227	2	16
β-strand	229-230	2	17
β-strand	239	1	17
β-strand	243-246	4	16

Chain Y: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	7-22	16
α-helix	30-43	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
mGs	A	protein	361	Homo sapiens	P63092 (AlphaFold model), P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	356	Homo sapiens	P62873 (AlphaFold model)
Soluble cytochrome b562,Trace amine-associated receptor 1	R	protein	470	Escherichia coli, Homo sapiens	P0ABE7 (AlphaFold model), Q96RJ0
ScFv16	S	protein	251	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	Y	protein	71	Mus musculus	P63213

Sequence of entity 1 (A), FASTA

>8JSO_1 mGs (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 2 (B), FASTA

>8JSO_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQ
MRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCA
YAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDT
TCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTG
HESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRL
LLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (R), FASTA

>8JSO_3 Soluble cytochrome b562,Trace amine-associated receptor 1 (chains R)
DYKDDDDKHHHHHHHHHHENLYFQGADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRA
AALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLK
TTRNAYIQKYLMMPFCHNIINISCVKNNWSNDVRASLYSLMVLIILTTLVGNLIVIVSIS
HFKQLHTPTNWLIHSMATVDFLLGCLVMPYSMVRSAEHCWYFGEVFCKIHTSTDIMLSSA
SIFHLSFISIDRYYAVCDPLRYKAKMNILVICVMIFISWSVPAVFAFGMIFLELNFKGAE
EIYYKHVHCRGGCSVFFSKISGVLTFMTSFYIPGSIMLCVYYRIYLIAKEQARLISDANQ
KLQIGLEMKNGISQSKERKAVKTLGIVMGVFLICWCPFFICTVMDPFLHYIIPPTLNDVL
IWFGYLNSTFNPMVYAFFYPWFRKALKMMLFGKIFQKDSSRCKLFLELSS

Sequence of entity 4 (S), FASTA

>8JSO_4 ScFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGSSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAA

Sequence of entity 5 (Y), FASTA

>8JSO_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains Y)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
1WE	(2S)-1-phenylpropan-2-amine	C9 H13 N	1

Primary citation

Ligand recognition and G-protein coupling of trace amine receptor TAAR1. Xu, Z., Guo, L., Yu, J. et al. Nature (2023) 624:672-681. DOI 10.1038/s41586-023-06804-z · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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