8OJ4: MlaCD complex

Structure of the MlaCD complex (1:6 stoichiometry). Determined by electron microscopy at 4.35 Å resolution. Released 10 Jul 2024.

Method: Electron microscopy
Resolution: 4.35 Å
Organism: Escherichia coli
Chains: 7
Atoms: 6,983
Mol. weight: 141.55 kDa
Released: 10 Jul 2024

Explore 8OJ4 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8OJ4 contains 21 α-helices and 61 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 10 β-strands

Element	Residues	Length	Sheet
β-strand	39-45	7	2
β-strand	60	1	3
β-strand	63	1	3
β-strand	66-73	8	2
β-strand	80-87	8	2
β-strand	98-103	6	4
β-strand	110-115	6	4
β-strand	120	1	5
β-strand	124	1	5
β-strand	133	1	2
α-helix	143-149	7

Chain B: 2 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	40-45	6	6
α-helix	52-53	2
β-strand	57-60	4	6
β-strand	63-73	11	6
β-strand	80-86	7	6
β-strand	97-103	7	6
β-strand	110-116	7	6
β-strand	133	1	6
α-helix	143-150	8

Chain C: 2 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	39-45	7	7
α-helix	52-53	2
β-strand	58-60	3	8
β-strand	63-64	2	8
β-strand	66-73	8	7
β-strand	80-87	8	7
β-strand	94	1	9
β-strand	97-103	7	8
β-strand	110-116	7	8
β-strand	120	1	10
β-strand	124	1	10
β-strand	127	1	9
β-strand	133	1	7
α-helix	143-149	7

Chain D: 1 helix, 8 β-strands

Element	Residues	Length	Sheet
β-strand	39-45	7	11
β-strand	57-60	4	12
β-strand	63-67	5	12
β-strand	68-72	5	11
β-strand	81-87	7	11
β-strand	98-103	6	12
β-strand	110-115	6	12
β-strand	132-133	2	11
α-helix	145-149	5

Chain E: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	40-45	6	13
β-strand	57-60	4	13
β-strand	63-72	10	13
β-strand	81-86	6	13
β-strand	94	1	14
β-strand	98-102	5	13
β-strand	111-115	5	13
β-strand	120	1	15
β-strand	124	1	15
β-strand	127	1	14
α-helix	128-129	2
β-strand	133	1	13
α-helix	145-149	5

Chain F: 1 helix, 9 β-strands

Element	Residues	Length	Sheet
β-strand	39-45	7	16
β-strand	57-60	4	17
β-strand	63-67	5	17
β-strand	68-72	5	16
β-strand	81-87	7	16
β-strand	97-103	7	17
β-strand	110-116	7	17
β-strand	133	1	16
β-strand	137	1	17
α-helix	145-149	5

Chain H: 12 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	27-43	17
α-helix	47-51	5
α-helix	53-55	3
α-helix	56-62	7
α-helix	65-67	3
β-strand	68	1	1
α-helix	71-74	4
α-helix	80-84	5
α-helix	87-108	22
α-helix	119-122	4
β-strand	130-135	6	1
β-strand	148-153	6	1
β-strand	162-163	2	1
α-helix	175-180	6
α-helix	183-187	5
α-helix	190-196	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Intermembrane phospholipid transport system binding protein MlaC	H	protein	211	Escherichia coli	P0ADV7 (AlphaFold model)
Intermembrane phospholipid transport system binding protein MlaD	A, B, C, D, E, F	protein	183	Escherichia coli	P64604 (AlphaFold model)

Sequence of entity 1 (H), FASTA

>8OJ4_1 Intermembrane phospholipid transport system binding protein MlaC (chains H)
MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKNEQPQIRANPDYLRTIV
DQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLKQAYGQALAMYHGQTYQIA
PEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGNWQAYDMIAEGVSMITTKQNE
WGTLLRTKGIDGLTAQLKSISQQKITLEEKK

Sequence of entity 2 (A, B, C, D, E, F), FASTA

>8OJ4_2 Intermembrane phospholipid transport system binding protein MlaD (chains A, B, C, D, E, F)
MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKARSPVSI
GGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFED
PELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNKNSGDAPAAAPGNNETTEPVG
TTK

Primary citation

Structure of the MlaC-MlaD complex reveals molecular basis of periplasmic phospholipid transport. Wotherspoon, P., Johnston, H., Hardy, D.J. et al. Nat Commun (2024) 15:6394-6394. DOI 10.1038/s41467-024-50615-3 · PubMed

Other PDB entries of the same protein (UniProt P0ADV7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5UWA 1.5 Å, Structure of E. coli phospholipid binding protein MlaC
6GKI 2.23 Å, Structure of E coli MlaC in Variously Loaded States
7VR6 2.5 Å, Crystal structure of MlaC from Escherichia coli in quasi-open state
8I8X 3.25 Å, Cryo-EM Structure of OmpC3-MlaA-MlaC Complex in MSP2N2 Nanodiscs
8OJG 4.38 Å, Structure of the MlaCD complex (2:6 stoichiometry)

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