8P0L: Human O-GlcNAcase

Crystal structure of human O-GlcNAcase in complex with an S-linked CKII peptide. Determined by X-ray diffraction at 2.5 Å resolution. Released 29 Nov 2023.

Method: X-ray diffraction
Resolution: 2.5 Å
Organism: Homo sapiens
Chains: 2
Atoms: 7,000
Mol. weight: 206.49 kDa
Ligands: NAG, SER, CYS
Released: 29 Nov 2023

Explore 8P0L in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8P0L contains 49 α-helices and 22 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 23 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	61-66	6	1
α-helix	72-74	3
α-helix	75-87	13
β-strand	92-95	4	1
α-helix	101-103	3
α-helix	110-112	3
α-helix	113-128	16
β-strand	132-137	6	1
α-helix	148-162	15
β-strand	168-172	5	1
α-helix	182-187	6
α-helix	191-205	15
β-strand	212-215	4	1
α-helix	221-223	3
α-helix	232-240	9
β-strand	246-249	4	1
β-strand	259	1	2
α-helix	261-271	11
α-helix	274-275	2
β-strand	276-279	4	1
β-strand	299	1	2
α-helix	301-306	6
β-strand	309-312	4	1
α-helix	318-320	3
α-helix	322-334	13
α-helix	376-391	16
α-helix	545-547	3
α-helix	555-564	10
β-strand	567	1	3
β-strand	570	1	3
α-helix	573-583	11
α-helix	587-591	5
α-helix	602-628	27
α-helix	634-661	28
α-helix	684-692	9

Chain B: 26 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	61-66	6	4
α-helix	72-74	3
α-helix	75-87	13
β-strand	92-95	4	4
α-helix	110-111	2
α-helix	113-128	16
β-strand	132-137	6	4
α-helix	148-162	15
β-strand	168-172	5	4
α-helix	182-187	6
α-helix	191-205	15
β-strand	212-215	4	4
α-helix	221-223	3
α-helix	232-240	9
β-strand	246-249	4	4
α-helix	261-271	11
α-helix	274-275	2
β-strand	276-279	4	4
α-helix	295	1
α-helix	301-306	6
β-strand	309-312	4	4
α-helix	318-321	4
α-helix	322-333	12
α-helix	376-389	14
α-helix	390-392	3
α-helix	545-547	3
α-helix	552-553	2
α-helix	555-564	10
β-strand	567	1	5
β-strand	570	1	5
α-helix	573-587	15
α-helix	588-590	3
α-helix	604-628	25
α-helix	634-661	28
α-helix	684-690	7
α-helix	708-710	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein O-GlcNAcase	A, B	protein	916	Homo sapiens	O60502 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>8P0L_1 Protein O-GlcNAcase (chains A, B)
MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRF
LCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLM
TLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNM
CAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEK
LLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRS
TELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLE
NEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAA
PSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKND
NQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVP
GPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKD
SEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQW
LGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPY
FPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYA
LGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFP
SLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIA
KMEGFPKDVVILGRSL

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	1
SER	Serine	C3 H7 N O3	1
CYS	Cysteine	C3 H7 N O2 S	1

Primary citation

Human O -GlcNAcase Uses a Preactivated Boat-skew Substrate Conformation for Catalysis. Evidence from X-ray Crystallography and QM/MM Metadynamics. Calvelo, M., Males, A., Alteen, M.G. et al. ACS Catal (2023) 13:13672-13678. DOI 10.1021/acscatal.3c02378 · PubMed

Other PDB entries of the same protein (UniProt O60502 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5UN8 2.13 Å, Crystal Structure of human O-GlcNAcase in complex with glycopeptide p53
5M7U 2.3 Å, Structure of human O-GlcNAc hydrolase with new iminocyclitol type inhibitor
11LJ 2.33 Å, Human oga in complex with ligand 24
5M7R 2.35 Å, Structure of human O-GlcNAc hydrolase
5M7S 2.4 Å, Structure of human O-GlcNAc hydrolase with bound transition state analog ThiametG
7OU6 2.41 Å, Human O-GlcNAc hydrolase in complex with DNJNAc-thiazolidines
5TKE 2.48 Å, Crystal Structure of Eukaryotic Hydrolase
5UN9 2.5 Å, The crystal structure of human O-GlcNAcase in complex with Thiamet-G
9BA8 2.54 Å, O-GlcNAcase (OGA) inhibitor complex for the Treatment of Alzheimer's Disease
2YDQ 2.6 Å, CpOGA D298N in complex with hOGA-derived O-GlcNAc peptide
5M7T 2.6 Å, Structure of human O-GlcNAc hydrolase with PugNAc type inhibitor
5VVO 2.6 Å, Structural Investigations of the Substrate Specificity of Human O-GlcNAcase

Browse structure collections

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