8R23: INTS9-INTS11-BRAT1 complex

INTS9-INTS11-BRAT1 complex. Determined by electron microscopy at 3.2 Å resolution. Released 31 Jul 2024.

Method: Electron microscopy
Resolution: 3.2 Å
Organism: Homo sapiens
Chains: 3
Atoms: 11,979
Mol. weight: 231.79 kDa
Ligands: ZN
Released: 31 Jul 2024

Explore 8R23 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8R23 contains 74 α-helices and 53 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 45 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	3-6	4
α-helix	9-17	9
α-helix	22-25	4
α-helix	26-42	17
α-helix	46-51	6
α-helix	55-62	8
α-helix	73-83	11
α-helix	87-92	6
α-helix	99-102	4
α-helix	105-109	5
α-helix	117-130	14
α-helix	134-142	9
α-helix	146-152	7
β-strand	157	1	1
α-helix	158-175	18
α-helix	193-206	14
α-helix	211-227	17
α-helix	231-245	15
α-helix	256-267	12
α-helix	270-273	4
α-helix	278-289	12
α-helix	295-303	9
α-helix	310-320	11
α-helix	322-329	8
β-strand	331	1	2
α-helix	348-356	9
α-helix	358-374	17
α-helix	387-402	16
β-strand	407	1	2
α-helix	410-419	10
α-helix	423-435	13
α-helix	443-457	15
α-helix	466-478	13
α-helix	494-508	15
α-helix	514-530	17
α-helix	535-542	8
α-helix	546-549	4
α-helix	559-574	16
α-helix	593-601	9
α-helix	607-622	16
α-helix	631-643	13
α-helix	648-665	18
α-helix	688-697	10
α-helix	700-708	9
α-helix	713-732	20
α-helix	769-778	10
α-helix	782-788	7
α-helix	799-807	9

Chain B: 14 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	2-7	6	3
β-strand	14-18	5	3
β-strand	23-26	4	3
α-helix	32-34	3
β-strand	83	1	4
β-strand	98-99	2	3
α-helix	104-106	3
α-helix	110-114	5
β-strand	122-123	2	3
β-strand	124-126	3	5
α-helix	127-144	18
β-strand	179	1	4
α-helix	184-190	7
β-strand	195-197	3	5
β-strand	212-213	2	6
β-strand	225-227	3	6
β-strand	234-238	5	6
α-helix	253-255	3
β-strand	260-264	5	6
α-helix	274-289	16
β-strand	295-298	4	7
α-helix	304-319	16
β-strand	326-327	2	7
α-helix	334-336	3
β-strand	373-374	2	7
β-strand	392-395	4	7
α-helix	405-411	7
β-strand	419-422	4	7
α-helix	434-436	3
β-strand	442-445	4	7
α-helix	454-464	11
β-strand	468-470	3	6
β-strand	472	1	8
α-helix	473-476	4
α-helix	495-496	2
β-strand	499	1	8
β-strand	503-504	2	3

Chain C: 15 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	4-10	7	9
β-strand	19-23	5	9
β-strand	28-31	4	9
α-helix	48-50	3
α-helix	57-60	4
β-strand	61-65	5	9
α-helix	71-74	4
α-helix	77-80	4
β-strand	90-91	2	9
β-strand	93	1	10
α-helix	94-111	18
α-helix	123-130	8
β-strand	133	1	9
β-strand	136	1	10
β-strand	142-144	3	11
β-strand	148-151	4	11
β-strand	154	1	12
β-strand	163-167	5	11
β-strand	172-176	5	11
β-strand	191	1	12
β-strand	199-203	5	11
α-helix	216-229	14
β-strand	234-236	3	13
α-helix	246-257	12
β-strand	265-267	3	13
α-helix	278-282	5
β-strand	305-306	2	13
α-helix	310-312	3
β-strand	319-322	4	13
α-helix	332-339	8
β-strand	347-349	3	13
α-helix	353-355	3
α-helix	359-364	6
β-strand	369	1	14
β-strand	379	1	14
β-strand	383-385	3	13
α-helix	395-405	11
β-strand	410-413	4	11
α-helix	417-429	13
β-strand	435-436	2	11
β-strand	442	1	1
β-strand	444-446	3	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
BRCA1-associated ATM activator 1	A	protein	827	Homo sapiens	Q6PJG6 (AlphaFold model)
Integrator complex subunit 9	B	protein	658	Homo sapiens	Q9NV88 (AlphaFold model)
Integrator complex subunit 11	C	protein	612	Homo sapiens	Q5TA45 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8R23_1 BRCA1-associated ATM activator 1 (chains A)
GPSQSNMDPECAQLLPALCAVLVDPRQPVADDTCLEKLLDWFKTVTEGESSVVLLQEHPC
LVELLSHVLKVQDLSSGVLSFSLRLAGTFAAQENCFQYLQQGELLPGLFGEPGPLGRATW
AVPTVRSGWIQGLRSLAQHPSALRFLADHGAVDTIFSLQGDSSLFVASAASQLLVHVLAL
SMRGGAEGQPCLPGGDWPACAQKIMDHVEESLCSAATPKVTQALNVLTTTFGRCQSPWTE
ALWVRLSPRVACLLERDPIPAAHSFVDLLLCVARSPVFSSSDGSLWETVARALSCLGPTH
MGPLALGILKLEHCPQALRTQAFQVLLQPLACVLKATVQAPGPPGLLDGTADDATTVDTL
LASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVGGH
LCGTLAGCVRVQRAALDFLGTLSQGTGPQELVTQALAVLLECLESPGSSPTVLKKAFQAT
LRWLLSSPKTPGCSDLGPLIPQFLRELFPVLQKRLCHPCWEVRDSALEFLTQLSRHWGGQ
ADFRCALLASEVPQLALQLLQDPESYVRASAVTAMGQLSSQGLHAPTSPEHAEARQSLFL
ELLHILSVDSEGFPRRAVMQVFTEWLRDGHADAAQDTEQFVATVLQAASRDLDWEVRAQG
LELALVFLGQTLGPPRTHCPYAVALPEVAPAQPLTEALRALCHVGLFDFAFCALFDCDRP
VAQKSCDLLLFLRDKIASYSSLREARGSPNTASAEATLPRWRAGEQAQPPGDQEPEAVLA
MLRSLDLEGLRSTLAESSDHVEKSPQSLLQDMLATGGFLQGDEADCY

Sequence of entity 2 (B), FASTA

>8R23_2 Integrator complex subunit 9 (chains B)
MKLYCLSGHPTLPCNVLKFKSTTIMLDCGLDMTSTLNFLPLPLVQSPRLSNLPGWSLKDG
NAFLDKELKECSGHVFVDSVPEFCLPETELIDLSTVDVILISNYHCMMALPYITEHTGFT
GTVYATEPTVQIGRLLMEELVNFIERVPKAQSASLWKNKDIQRLLPSPLKDAVEVSTWRR
CYTMQEVNSALSKIQLVGYSQKIELFGAVQVTPLSSGYALGSSNWIIQSHYEKVSYVSGS
SLLTTHPQPMDQASLKNSDVLVLTGLTQIPTANPDGMVGEFCSNLALTVRNGGNVLVPCY
PSGVIYDLLECLYQYIDSAGLSSVPLYFISPVANSSLEFSQIFAEWLCHNKQSKVYLPEP
PFPHAELIQTNKLKHYPSIHGDFSNDFRQPCVVFTGHPSLRFGDVVHFMELWGKSSLNTV
IFTEPDFSYLEALAPYQPLAMKCIYCPIDTRLNFIQVSKLLKEVQPLHVVCPEQYTQPPP
AQSHRMDLMIDCQPPAMSYRRAEVLALPFKRRYEKIEIMPELADSLVPMEIKPGISLATV
SAVLHTKDNKHLLQPPPRPAQPTSGKKRKRVSDDVPDCKVLKPLLSGSIPVEQFVQTLEK
HGFSDIKVEDTAKGHIVLLQEAETLIQIEEDSTHIICDNDEMLRVRLRDLVLKFLQKF

Sequence of entity 3 (C), FASTA

>8R23_3 Integrator complex subunit 11 (chains C)
GPSDPGPKRAEFMPEIRVTPLGAGQDVGRSCILVSIAGKNVMLDCGMHMGFNDDRRFPDF
SYITQNGRLTDFLDCVIISHFHLDHCGALPYFSEMVGYDGPIYMTHPTQAICPILLEDYR
KIAVDKKGEANFFTSQMIKDCMKKVVAVHLHQTVQVDDELEIKAYYAGHVLGAAMFQIKV
GSESVVYTGDYNMTPDRHLGAAWIDKCRPNLLITESTYATTIRDSKRCRERDFLKKVHET
VERGGKVLIPVFALGRAQELCILLETFWERMNLKVPIYFSTGLTEKANHYYKLFIPWTNQ
KIRKTFVQRNMFEFKHIKAFDRAFADNPGPMVVFATPGMLHAGQSLQIFRKWAGNEKNMV
IMPGYCVQGTVGHKILSGQRKLEMEGRQVLEVKMQVEYMSFSAHADAKGIMQLVGQAEPE
SVLLVHGEAKKMEFLKQKIEQELRVNCYMPANGETVTLPTSPSIPVGISLGLLKREMAQG
LLPEAKKPRLLHGTLIMKDSNFRLVSSEQALKELGLAEHQLRFTCRVHLHDTRKEQETAL
RVYSHLKSVLKDHCVQHLPDGSVTVESVLLQAAAPSEDPGTKVLLVSWTYQDEELGSFLT
SLLKKGLPQAPS

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	2

Primary citation

Assembly mechanism of Integrator's RNA cleavage module. Sabath, K., Qiu, C., Jonas, S. Mol Cell (2024) 84:2882-2899.e10. DOI 10.1016/j.molcel.2024.06.032 · PubMed

Other PDB entries of the same protein (UniProt Q6PJG6 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4IFI 2.2 Å, Structure of human BRCA1 BRCT in complex with BAAT peptide
8UIB 3.21 Å, Structure of the human INTS9-INTS11-BRAT1 complex
8R22 3.9 Å, INTS9-INTS11-BRAT1-WDR73 complex
8R2D 3.9 Å, Integrator cleavage module assembly intermediate

Browse structure collections

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