8R86: Endo-1,4-beta-xylanase

Xylanase from Bacillus circulans mutant E78Q/W71A. Determined by X-ray diffraction at 1.5 Å resolution. Released 21 Aug 2024.

Method: X-ray diffraction
Resolution: 1.5 Å
Organism: Niallia circulans subsp. circulans
Chains: 2
Atoms: 3,533
Mol. weight: 40.59 kDa
Released: 21 Aug 2024

Explore 8R86 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8R86 contains 8 α-helices and 24 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	5-10	6	1
β-strand	15-20	6	2
β-strand	25-31	7	2
β-strand	35-42	8	1
α-helix	49	1
β-strand	50-61	12	2
β-strand	64-72	9	1
β-strand	77-85	9	1
β-strand	93-100	8	1
β-strand	103-113	11	1
α-helix	114-116	3
β-strand	124-132	9	1
α-helix	135-137	3
β-strand	142-145	4	2
α-helix	146-155	10
α-helix	159-161	3
β-strand	164-174	11	1
β-strand	177-184	8	2

Chain B: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	5-10	6	3
β-strand	15-20	6	3
β-strand	25-31	7	3
β-strand	35-42	8	3
β-strand	50-73	24	3
β-strand	77-85	9	3
β-strand	93-100	8	3
β-strand	103-113	11	3
α-helix	114-117	4
β-strand	124-132	9	3
α-helix	135-137	3
β-strand	142-145	4	3
α-helix	146-155	10
β-strand	163-184	22	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Endo-1,4-beta-xylanase	A, B	protein	185	Niallia circulans subsp. circulans	P09850 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>8R86_1 Endo-1,4-beta-xylanase (chains A, B)
ASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGNFVVGKGWTTGSPFRTINYNAGVWAP
NGNGYLTLYGATRSPLIQYYVVDSWGTYRPTGTYKGTVKSDGGTYDIYTTTRYNAPSIDG
DRTTFTQYWSVRQSKRPTGSNATITFTNHVNAWKSHGMNLGSNWAYQVMATEGYQSSGSS
NVTVW

Primary citation

Bimodal substrate binding in the active site of the glycosidase BcX. Saberi, M., Chikunova, A., Ben Bdira, F. et al. FEBS J (2024) 291:4222-4239. DOI 10.1111/febs.17251 · PubMed

Other PDB entries of the same protein (UniProt P09850 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QY3 1.24 Å, Xylanase from Bacillus circulans mutant E78Q/F125A bound to xylotriose
8R85 1.3 Å, Xylanase from Bacillus circulans mutant E78Q/W9A
8QXY 1.41 Å, Xylanase from Bacillus circulans mutant E78Q bound to xylotriose
1XNB 1.49 Å, High-resolution structures of xylanases from B. Circulans and T. Harzianum identify a…
2BVV 1.5 Å, Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase.
8QXZ 1.5 Å, Xylanase from Bacillus circulans mutant E78Q/Y69A
1C5H 1.55 Å, Hydrogen bonding and catalysis: an unexpected explanation for how a single amino acid…
3VZK 1.55 Å, Crystal structure of the Bacillus circulans endo-beta-(1,4)-xylanase (BcX) N35E mutant
1HV0 1.6 Å, Dissecting electrostatic interactions and the PH-dependent activity of a family 11…
1XNC 1.6 Å, Thermostabilization of the bacillus circulans xylanase, by the introduction of disulfide…
3VZN 1.67 Å, Crystal structure of the Bacillus circulans endo-beta-(1,4)-xylanase (BcX) N35E mutant…
8QY2 1.7 Å, Xylanase from Bacillus circulans mutant E78Q/F125A

Browse structure collections

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