8RVO: Proteasome subunit beta type-6

Proteasomal late precursor complex from pre1-1, state 1. Determined by electron microscopy at 2.69 Å resolution. Released 11 Sept 2024.

Method: Electron microscopy
Resolution: 2.69 Å
Organism: Saccharomyces cerevisiae
Chains: 34
Atoms: 60,650
Mol. weight: 927.16 kDa
Released: 11 Sept 2024

Explore 8RVO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8RVO contains 306 α-helices and 446 β-strands across 34 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 1: 6 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	11-16	6	1
β-strand	20-26	7	1
β-strand	29-31	3	2
β-strand	34-37	4	2
β-strand	39	1	3
β-strand	43-47	5	4
β-strand	50-56	7	4
α-helix	58-78	21
α-helix	82-85	4
α-helix	86-98	13
β-strand	107-114	8	4
β-strand	120-125	6	4
β-strand	131-134	4	4
β-strand	136-140	5	1
α-helix	143-153	11
β-strand	155	1	5
β-strand	171	1	5
α-helix	172-174	3
α-helix	177-194	18
β-strand	202-208	7	1
β-strand	211-217	7	1

Chain 2: 8 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	6-7	2	9
β-strand	11-16	6	10
β-strand	19-25	7	10
β-strand	28-30	3	9
β-strand	33-36	4	9
β-strand	42-45	4	3
β-strand	49-55	7	3
α-helix	57-74	18
α-helix	86-88	3
α-helix	89-105	17
β-strand	112-119	8	3
β-strand	125-130	6	3
β-strand	136-138	3	3
β-strand	141-143	3	10
α-helix	145-148	4
α-helix	151-156	6
α-helix	162-164	3
α-helix	170-186	17
β-strand	194-201	8	10
β-strand	207-213	7	10
α-helix	220-223	4

Chain 3: 9 helices, 3 β-strands

Element	Residues	Length	Sheet
β-strand	12-13	2	15
β-strand	22	1	16
β-strand	30	1	16
α-helix	32-35	4
α-helix	43-47	5
α-helix	52-58	7
α-helix	61-75	15
α-helix	79-94	16
α-helix	102-104	3
α-helix	105-111	7
α-helix	119-122	4
α-helix	138-146	9

Chain 4: 10 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	9-12	4
α-helix	18-20	3
α-helix	30-32	3
β-strand	35-37	3	19
α-helix	44-46	3
β-strand	49-54	6	19
α-helix	55-60	6
β-strand	69-78	10	19
β-strand	118-127	10	19
β-strand	130-135	6	19
α-helix	144-158	15
β-strand	164-171	8	19
β-strand	178-181	4	19
β-strand	193-194	2	20
α-helix	196-206	11
β-strand	215-220	6	19
β-strand	222	1	21
β-strand	229	1	21
α-helix	232-246	15
α-helix	250-262	13
α-helix	266-268	3
β-strand	275	1	22

Chain 5: 13 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-5	2	27
β-strand	6-7	2	28
α-helix	11-13	3
α-helix	14-24	11
β-strand	30-35	6	27
α-helix	36-37	2
β-strand	41	1	29
α-helix	42-43	2
β-strand	44-45	2	20
β-strand	46	1	30
β-strand	51	1	31
α-helix	55-57	3
β-strand	67	1	30
β-strand	70-75	6	27
β-strand	80-85	6	27
β-strand	89	1	29
α-helix	92-94	3
α-helix	95-98	4
α-helix	99-103	5
α-helix	104-110	7
β-strand	117-121	5	28
β-strand	131	1	32
β-strand	138-142	5	28
β-strand	164-165	2	33
β-strand	169-170	2	28
β-strand	176	1	32
β-strand	179-180	2	33
α-helix	184-193	10
β-strand	206-211	6	28
α-helix	218-230	13
α-helix	237-239	3
β-strand	246	1	31
α-helix	247-251	5
β-strand	266	1	34

Chain 6: 10 helices, 3 β-strands

Element	Residues	Length	Sheet
β-strand	12-13	2	38
α-helix	19-21	3
β-strand	22	1	39
β-strand	30	1	39
α-helix	32-35	4
α-helix	43-47	5
α-helix	54-58	5
α-helix	60-75	16
α-helix	79-93	15
α-helix	102-104	3
α-helix	105-110	6
α-helix	119-122	4
α-helix	138-146	9

Chain 7: 11 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	10-12	3
α-helix	18-20	3
α-helix	30-32	3
β-strand	35-37	3	42
α-helix	44-46	3
β-strand	49-54	6	42
α-helix	55-60	6
α-helix	63-66	4
β-strand	69-78	10	42
β-strand	118-127	10	42
β-strand	130-135	6	42
α-helix	144-158	15
β-strand	164-171	8	42
β-strand	178-181	4	42
β-strand	193-194	2	43
α-helix	196-206	11
β-strand	214-220	7	42
β-strand	222	1	44
β-strand	229	1	44
α-helix	232-246	15
α-helix	250-262	13
α-helix	271-274	4
β-strand	275	1	45

Chain 8: 12 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	50
α-helix	11-13	3
α-helix	14-25	12
β-strand	31-35	5	50
α-helix	36-37	2
β-strand	41	1	51
β-strand	44-45	2	43
β-strand	46-47	2	52
β-strand	51	1	53
α-helix	55-57	3
β-strand	66-67	2	52
β-strand	71-75	5	50
β-strand	80-84	5	50
α-helix	88	1
β-strand	89	1	51
α-helix	95-98	4
α-helix	99-103	5
α-helix	104-106	3
β-strand	114-121	8	50
β-strand	138-144	7	50
β-strand	169-170	2	50
α-helix	184-194	11
β-strand	204-211	8	50
α-helix	218-231	14
β-strand	246	1	53
α-helix	247-251	5
α-helix	258-261	4
β-strand	266	1	54

26 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Proteasome subunit beta type-6	1, M	protein	241	Saccharomyces cerevisiae	P23724 (AlphaFold model)
Proteasome subunit beta type-7	2, N	protein	266	Saccharomyces cerevisiae	P30657 (AlphaFold model)
Proteasome maturation factor UMP1	3, 6	protein	148	Saccharomyces cerevisiae	P38293 (AlphaFold model)
Proteasome chaperone 1	4, 7	protein	276	Saccharomyces cerevisiae	Q05778 (AlphaFold model)
Proteasome assembly chaperone 2	5, 8	protein	267	Saccharomyces cerevisiae	P36040
Proteasome subunit alpha type-1	A, O	protein	252	Saccharomyces cerevisiae	P21243
Proteasome subunit alpha type-2	B, P	protein	250	Saccharomyces cerevisiae	P23639
Proteasome subunit alpha type-3	C, Q	protein	258	Saccharomyces cerevisiae	P23638
Proteasome subunit alpha type-4	D, R	protein	254	Saccharomyces cerevisiae	P40303
Proteasome subunit alpha type-5	E, S	protein	260	Saccharomyces cerevisiae	P32379
Proteasome subunit alpha type-6	F, T	protein	234	Saccharomyces cerevisiae	P40302
Probable proteasome subunit alpha type-7	G, U	protein	288	Saccharomyces cerevisiae	P21242

5 more molecules are not listed.

Sequence of entity 1 (1, M), FASTA

>8RVO_1 Proteasome subunit beta type-6 (chains 1, M)
MATIASEYSSEASNTPIEHQFNPYGDNGGTILGIAGEDFAVLAGDTRNITDYSINSRYEP
KVFDCGDNIVMSANGFAADGDALVKRFKNSVKWYHFDHNDKKLSINSAARNIQHLLYGKR
FFPYYVHTIIAGLDEDGKGAVYSFDPVGSYEREQCRAGGAAASLIMPFLDNQVNFKNQYE
PGTNGKVKKPLKYLSVEEVIKLVRDSFTSATERHIQVGDGLEILIVTKDGVRKEFYELKR
D

Sequence of entity 2 (2, N), FASTA

>8RVO_2 Proteasome subunit beta type-7 (chains 2, N)
MNHDPFSWGRPADSTYGAYNTQIANAGASPMVNTQQPIVTGTSVISMKYDNGVIIAADNL
GSYGSLLRFNGVERLIPVGDNTVVGISGDISDMQHIERLLKDLVTENAYDNPLADAEEAL
EPSYIFEYLATVMYQRRSKMNPLWNAIIVAGVQSNGDQFLRYVNLLGVTYSSPTLATGFG
AHMANPLLRKVVDRESDIPKTTVQVAEEAIVNAMRVLYYRDARSSRNFSLAIIDKNTGLT
FKKNLQVENMKWDFAKDIKGYGTQKI

Sequence of entity 3 (3, 6), FASTA

>8RVO_3 Proteasome maturation factor UMP1 (chains 3, 6)
MNIVPQDTFKSQVSTDQDKSVLSSAVPSLPDTLRQQEGGAVPLSTQLNDRHPLESTLKNW
ETTQRQRQMEQYRQIFGIAEPMKRTMEMEIVNRTDFNPLSTNGSIHRDILLNKECSIDWE
DVYPGTGLQASTMVGDDVHSKIEKQLGI

Sequence of entity 4 (4, 7), FASTA

>8RVO_4 Proteasome chaperone 1 (chains 4, 7)
MLFKQWNDLPEPKHLLDLPEISKNLQSLEVCPVPKVEFPQDLDVPQYSTAVITTKIMNPL
FPKNLLQLTSIGEIKTTLTVKSPSLPQSSGKHSWNYDENFPNEVDPDQKNDTADETVYGF
SFPIYSFGKTLLFSMEENFISISPIFGNMISRSIISQLAQFSPDIIVIGTSDKIASMKVM
TENECTLQPPEFITGFIGSVLTQLIVGPSKGLKFKCLVAPSEGPNGFEKLSLSDMGSLVD
LCGQWLGFEPSRYSEECYRLWRCDSAAIGAQSGLYI

Sequence of entity 5 (5, 8), FASTA

>8RVO_5 Proteasome assembly chaperone 2 (chains 5, 8)
MSCLVLPLVSVGNIPQLSIDWLLNSQANEWEYLEALDSKYLVEFVGPLDRPEDGSDSLYK
DADMKYSSALEVFYNKKRGLFAIQQRTPLVSVNYLNNFIVEIILPFLSKYNISEICIWDS
LYAMEDENGVIVRPQEVYSLGEFYFDDEAELLSNLHLNDQESMVNNWLHFTPTSFQDKIS
VDQPIFKILFQILNASQRPKALRSIKYCSCLANEGDNSLDSQQFLQWIISQKVIKNAPPI
VKFVRPISWQGAYGMADARDKFVDLYN

Sequence of entity 6 (A, O), FASTA

>8RVO_6 Proteasome subunit alpha type-1 (chains A, O)
MSGAAAASAAGYDRHITIFSPEGRLYQVEYAFKATNQTNINSLAVRGKDCTVVISQKKVP
DKLLDPTTVSYIFCISRTIGMVVNGPIPDARNAALRAKAEAAEFRYKYGYDMPCDVLAKR
MANLSQIYTQRAYMRPLGVILTFVSVDEELGPSIYKTDPAGYYVGYKATATGPKQQEITT
NLENHFKKSKIDHINEESWEKVVEFAITHMIDALGTEFSKNDLEVGVATKDKFFTLSAEN
IEERLVAIAEQD

Sequence of entity 7 (B, P), FASTA

>8RVO_7 Proteasome subunit alpha type-2 (chains B, P)
MTDRYSFSLTTFSPSGKLGQIDYALTAVKQGVTSLGIKATNGVVIATEKKSSSPLAMSET
LSKVSLLTPDIGAVYSGMGPDYRVLVDKSRKVAHTSYKRIYGEYPPTKLLVSEVAKIMQE
ATQSGGVRPFGVSLLIAGHDEFNGFSLYQVDPSGSYFPWKATAIGKGSVAAKTFLEKRWN
DELELEDAIHIALLTLKESVEGEFNGDTIELAIIGDENPDLLGYTGIPTDKGPRFRKLTS
QEINDRLEAL

Sequence of entity 8 (C, Q), FASTA

>8RVO_8 Proteasome subunit alpha type-3 (chains C, Q)
MGSRRYDSRTTIFSPEGRLYQVEYALESISHAGTAIGIMASDGIVLAAERKVTSTLLEQD
TSTEKLYKLNDKIAVAVAGLTADAEILINTARIHAQNYLKTYNEDIPVEILVRRLSDIKQ
GYTQHGGLRPFGVSFIYAGYDDRYGYQLYTSNPSGNYTGWKAISVGANTSAAQTLLQMDY
KDDMKVDDAIELALKTLSKTTDSSALTYDRLEFATIRKGANDGEVYQKIFKPQEIKDILV
KTGITKKDEDEEADEDMK

Sequence of entity 9 (D, R), FASTA

>8RVO_9 Proteasome subunit alpha type-4 (chains D, R)
MSGYDRALSIFSPDGHIFQVEYALEAVKRGTCAVGVKGKNCVVLGCERRSTLKLQDTRIT
PSKVSKIDSHVVLSFSGLNADSRILIEKARVEAQSHRLTLEDPVTVEYLTRYVAGVQQRY
TQSGGVRPFGVSTLIAGFDPRDDEPKLYQTEPSGIYSSWSAQTIGRNSKTVREFLEKNYD
RKEPPATVEECVKLTVRSLLEVVQTGAKNIEITVVKPDSDIVALSSEEINQYVTQIEQEK
QEQQEQDKKKKSNH

Sequence of entity 10 (E, S), FASTA

>8RVO_10 Proteasome subunit alpha type-5 (chains E, S)
MFLTRSEYDRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGIATKEGVVLGVEKRATSPLLE
SDSIEKIVEIDRHIGCAMSGLTADARSMIEHARTAAVTHNLYYDEDINVESLTQSVCDLA
LRFGEGASGEERLMSRPFGVALLIAGHDADDGYQLFHAEPSGTFYRYNAKAIGSGSEGAQ
AELLNEWHSSLTLKEAELLVLKILKQVMEEKLDENNAQLSCITKQDGFKIYDNEKTAELI
KELKEKEAAESPEEADVEMS

Sequence of entity 11 (F, T), FASTA

>8RVO_11 Proteasome subunit alpha type-6 (chains F, T)
MFRNNYDGDTVTFSPTGRLFQVEYALEAIKQGSVTVGLRSNTHAVLVALKRNADELSSYQ
KKIIKCDEHMGLSLAGLAPDARVLSNYLRQQCNYSSLVFNRKLAVERAGHLLCDKAQKNT
QSYGGRPYGVGLLIIGYDKSGAHLLEFQPSGNVTELYGTAIGARSQGAKTYLERTLDTFI
KIDGNPDELIKAGVEAISQSLRDESLTVDNLSIAIVGKDTPFTIYDGEAVAKYI

Sequence of entity 12 (G, U), FASTA

>8RVO_12 Probable proteasome subunit alpha type-7 (chains G, U)
MTSIGTGYDLSNSVFSPDGRNFQVEYAVKAVENGTTSIGIKCNDGVVFAVEKLITSKLLV
PQKNVKIQVVDRHIGCVYSGLIPDGRHLVNRGREEAASFKKLYKTPIPIPAFADRLGQYV
QAHTLYNSVRPFGVSTIFGGVDKNGAHLYMLEPSGSYWGYKGAATGKGRQSAKAELEKLV
DHHPEGLSAREAVKQAAKIIYLAHEDKKEKDFELEISWCSLSETNGLHKFVKGDLLQEAI
DFAQKEINGDDDEDEDDSDNVMSSDDENAPVATNANATTDQEGDIHLE

Primary citation

Structural roles of Ump1 and beta-subunit propeptides in proteasome biogenesis. Mark, E., Ramos, P.C., Kayser, F. et al. Life Sci Alliance (2024) 7. DOI 10.26508/lsa.202402865 · PubMed

Other PDB entries of the same protein (UniProt P23724 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1RYP 1.9 Å, Crystal structure of the 20S proteasome from yeast at 2.4 Å resolution
8RVQ 2.02 Å, 20S proteasome from pre1-1
4R17 2.1 Å, Ligand-induced aziridine-formation at subunit beta5 of the yeast 20S proteasome
8RVL 2.14 Å, Proteasomal late precursor complex from pre1-1
8U7U 2.16 Å, Proteasome 20S Core Particle from Beta 3 D205 deletion
1G65 2.25 Å, Crystal structure of epoxomicin:20s proteasome reveals a molecular basis for selectivity…
8RVP 2.28 Å, Proteasomal late precursor complex from pre1-1, state 2
4QVP 2.3 Å, yCP beta5-M45T mutant in complex with bortezomib
5CZ4 2.3 Å, Yeast 20S proteasome at 2.3 A resolution
6HWE 2.3 Å, Yeast 20S proteasome beta2-G45A mutant in complex with carfilzomib
9GBK 2.39 Å, Blm10-20S proteasome complex from pre1-1
1G0U 2.4 Å, A gated channel into the proteasome core particle

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