8T74: KRAS4a

Crystal structure of KRAS4a (GMPPNP) in complex with RAF1 (RBD). Determined by X-ray diffraction at 1.65 Å resolution. Released 14 Feb 2024.

Method: X-ray diffraction
Resolution: 1.65 Å
Organism: Homo sapiens
Chains: 2
Atoms: 2,172
Mol. weight: 30.08 kDa
Ligands: GNP, MG
Released: 14 Feb 2024

Explore 8T74 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8T74 contains 10 α-helices and 15 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	1
α-helix	16-24	9
β-strand	37-46	10	1
β-strand	49-58	10	1
α-helix	59	1
α-helix	62-67	6
α-helix	68-74	7
β-strand	77-83	7	1
α-helix	87-104	18
β-strand	111-116	6	1
α-helix	127-137	11
β-strand	141-143	3	1
β-strand	145	1	2
β-strand	150	1	2
α-helix	152-169	18

Chain B: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	57-62	6	1
β-strand	66-71	6	1
β-strand	77	1	3
α-helix	78-88	11
α-helix	93-95	3
β-strand	96-100	5	1
β-strand	110-112	3	1
β-strand	117	1	3
α-helix	118-121	4
β-strand	125-130	6	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
GTPase KRas	A	protein	178	Homo sapiens	P01116 (AlphaFold model)
RAF proto-oncogene serine/threonine-protein kinase	B	protein	80	Homo sapiens	P04049 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8T74_1 GTPase KRas (chains A)
GMTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTA
GQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCD
LPSRTVDTKQAQDLARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKT

Sequence of entity 2 (B), FASTA

>8T74_2 RAF proto-oncogene serine/threonine-protein kinase (chains B)
SKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKAR
LDWNTDAASLIGEELQVDFL

Ligands and cofactors

ID	Name	Formula	Copies
GNP	Phosphoaminophosphonic acid-guanylate ester	C10 H17 N6 O13 P3	1
MG	Magnesium ion	Mg	1

Primary citation

Comparative analysis of KRAS4a and KRAS4b splice variants reveals distinctive structural and functional properties. Whitley, M.J., Tran, T.H., Rigby, M. et al. Sci Adv (2024) 10:eadj4137-eadj4137. DOI 10.1126/sciadv.adj4137 · PubMed

Other PDB entries of the same protein (UniProt P01116 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9IAY 0.95 Å, Structure of 10 in complex with GDP-KRAS
9IAW 1.0 Å, Structure of 5 in complex with GDP-KRAS
12XE 1.01 Å, Crystal structure of KRAS(Q61K) bound to GppNHp and ligand
8ONV 1.01 Å, KRAS-G13D in complex with BI-2493
6P0Z 1.01 Å, Crystal structure of N-acetylated KRAS (2-169) bound to GDP and Mg
9IB5 1.01 Å, Structure of 18 (BI-2493) in complex with GDP-KRAS
8AZZ 1.02 Å, KRAS-G12V in complex with BI-2865
8AZX 1.04 Å, KRAS-G12C in complex with BI-2865
8B00 1.04 Å, KRAS-G13D in complex with BI-2865
8TVK 1.04 Å, KRAS 1-169 G12C Mutant at 100k
4QL3 1.04 Å, Crystal Structure of a GDP-bound G12R Oncogenic Mutant of Human GTPase KRas
8AZV 1.05 Å, KRAS in complex with BI-2865

Browse structure collections

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