8THW: Cac1 PIP motif

Cac1 PIP motif bound to PCNA. Determined by X-ray diffraction at 2.6 Å resolution. Released 18 Sept 2024.

Method: X-ray diffraction
Resolution: 2.6 Å
Organisms: Saccharomyces cerevisiae S288C, Saccharomyces cerevisiae
Chains: 3
Atoms: 6,315
Mol. weight: 97.17 kDa
Released: 18 Sept 2024

Explore 8THW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8THW contains 25 α-helices and 69 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	1
α-helix	10-17	8
β-strand	25-31	7	2
β-strand	34-40	7	2
β-strand	46-53	8	2
α-helix	54-56	3
β-strand	59-62	4	1
β-strand	66-71	6	2
α-helix	72-79	8
β-strand	87-92	6	1
β-strand	98-104	7	1
β-strand	111-117	7	1
β-strand	126	1	3
β-strand	135-140	6	2
α-helix	141-151	11
β-strand	157-162	6	4
β-strand	167-172	6	4
β-strand	176-182	7	4
β-strand	196-199	4	2
β-strand	203-208	6	4
α-helix	209-215	7
α-helix	216-220	5
β-strand	224-229	6	2
β-strand	235-241	7	2
β-strand	244-250	7	2
β-strand	253	1	4
β-strand	269	1	4
α-helix	272-274	3
β-strand	278	1	3

Chain B: 8 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	4
α-helix	10-20	11
β-strand	25-31	7	5
β-strand	34-40	7	5
β-strand	46-53	8	5
α-helix	54-56	3
β-strand	59	1	4
β-strand	62	1	4
β-strand	66-71	6	5
α-helix	72-78	7
β-strand	87-92	6	4
β-strand	98-104	7	4
β-strand	111-117	7	4
β-strand	126	1	6
β-strand	135-140	6	5
α-helix	141-151	11
β-strand	157-163	7	7
β-strand	166-172	7	7
β-strand	176-182	7	7
β-strand	185	1	8
α-helix	191-193	3
β-strand	195	1	8
β-strand	196-199	4	5
β-strand	203-208	6	7
α-helix	209-215	7
α-helix	216-221	6
β-strand	224-229	6	5
β-strand	235-241	7	5
β-strand	244-250	7	5
α-helix	272-274	3
β-strand	278	1	6

Chain C: 10 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	7
α-helix	9-17	9
β-strand	25-30	6	9
β-strand	34-40	7	9
β-strand	46-53	8	9
α-helix	54-56	3
β-strand	59-62	4	7
β-strand	66-71	6	9
α-helix	72-79	8
β-strand	87-92	6	7
β-strand	98-104	7	7
β-strand	110-117	8	7
β-strand	126	1	10
α-helix	127-129	3
β-strand	135-140	6	9
α-helix	141-151	11
β-strand	157-162	6	1
β-strand	167-172	6	1
β-strand	176-182	7	1
β-strand	185	1	11
β-strand	195	1	11
β-strand	198-199	2	9
β-strand	203-208	6	1
α-helix	209-215	7
α-helix	216-220	5
β-strand	224-229	6	9
β-strand	235-240	6	9
β-strand	245-250	6	9
β-strand	253	1	1
α-helix	268	1
β-strand	269	1	1
α-helix	270	1
α-helix	272-274	3
β-strand	278	1	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Proliferating cell nuclear antigen,Chromatin assembly factor 1 subunit p90	A, B, C	protein	289	Saccharomyces cerevisiae S288C, Saccharomyces cerevisiae	P15873 (AlphaFold model), Q12495 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>8THW_1 Proliferating cell nuclear antigen,Chromatin assembly factor 1 subunit p90 (chains A, B, C)
MHHHHHHMLEAKFEEASLFKRIIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIG
VEAFQEYRCDHPVTLGMDLTSLSKILRCGNNTDTLTLIADNTPDSIILLFEDTKKDRIAE
YSLKLMDIDADFLKIEELQYDSTLSLPSSEFSKIVRDLSQLSDSINIMITKETIKFVADG
DIGSGSVIIKPFVDMEHPETSIKLEMDQPVDLTFGAKYLLDIIKGSSLSDRVGIRLSSEA
PALFQFDLKSGFLQFFLAPKFNDEEGGSGGAKERAQSRIGNFFKKLSDS

Primary citation

Structural Basis for the Interaction Between Yeast Chromatin Assembly Factor 1 and Proliferating Cell Nuclear Antigen. Orndorff, K.S., Veltri, E.J., Hoitsma, N.M. et al. J Mol Biol (2024) 436:168695-168695. DOI 10.1016/j.jmb.2024.168695 · PubMed

Other PDB entries of the same protein (UniProt P15873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5V7M 1.93 Å, PCNA mutant L126A/I128A Protein Defective in Gene Silencing
8DQX 2.1 Å, Open state of RFC:PCNA bound to a 3' ss/dsDNA junction
8DR1 2.14 Å, Consensus closed state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2)
8DR3 2.2 Å, Closed state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2) with NTD
1PLQ 2.3 Å, Crystal structure of the eukaryotic DNA polymerase processivity factor pcna
8DR6 2.39 Å, Closed state of RFC:PCNA bound to a nicked dsDNA
8DR0 2.42 Å, Closed state of RFC:PCNA bound to a 3' ss/dsDNA junction
8DR4 2.45 Å, Open state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2) without NTD
3GPN 2.5 Å, Structure of the non-trimeric form of the E113G PCNA mutant protein
3V60 2.6 Å, Structure of S. cerevisiae PCNA conjugated to SUMO on lysine 164
6W9W 2.65 Å, R80A PCNA mutant defective in BIR
4L6P 2.68 Å, Structure of C22Y Mutant PCNA protein defective in DNA mismatch repair

Browse structure collections

About this viewer

MolViewer shows 8THW directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.