8U7Z: PDB entry 8U7Z

Name: PDB 8U7Z: KCTD5/Cullin3/Gbeta1gamma2 Complex: Local Refinment of KCTD5(CTD)/Gbeta1gamma2
Published: 2023-10-11
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: ligase

KCTD5/Cullin3/Gbeta1gamma2 Complex: Local Refinment of KCTD5(CTD)/Gbeta1gamma2. Determined by electron microscopy at 2.97 Å resolution. Released 11 Oct 2023.

Method: Electron microscopy
Resolution: 2.97 Å
Organism: Homo sapiens
Chains: 15
Atoms: 18,695
Mol. weight: 356.94 kDa
Released: 11 Oct 2023

Explore 8U7Z in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8U7Z contains 58 α-helices and 163 β-strands across 15 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B1: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	88-94	7	2
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
β-strand	134-139	6	3
β-strand	146-153	8	4
β-strand	156-161	6	4
β-strand	165-170	6	4
β-strand	175-180	6	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	208-212	5	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	249-254	6	6
β-strand	259-265	7	6
α-helix	272	1
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	304-308	5	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain B2: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
α-helix	45-46	2
β-strand	47	1	8
β-strand	58-63	6	9
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	88-94	7	9
β-strand	100-105	6	10
β-strand	111-116	6	10
β-strand	120-125	6	10
β-strand	134-140	7	10
β-strand	146-151	6	11
β-strand	156-161	6	11
β-strand	166-170	5	11
β-strand	175-180	6	11
α-helix	186	1
β-strand	187-192	6	12
β-strand	198-203	6	12
β-strand	208-212	5	12
β-strand	217-222	6	12
β-strand	229-234	6	13
β-strand	240-245	6	13
β-strand	250-254	5	13
β-strand	260-264	5	13
α-helix	272	1
β-strand	273-278	6	14
β-strand	284-289	6	14
β-strand	294-298	5	14
β-strand	304-308	5	14
β-strand	315-320	6	8
β-strand	327-331	5	8
β-strand	336-339	4	8

Chain B3: 6 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	15
β-strand	58-63	6	16
β-strand	69-74	6	16
β-strand	78-83	6	16
β-strand	88-94	7	16
β-strand	100-105	6	17
β-strand	111-116	6	17
β-strand	120-125	6	17
β-strand	134-140	7	17
β-strand	146-151	6	18
β-strand	156-161	6	18
β-strand	165	1	18
α-helix	167	1
β-strand	168-170	3	18
β-strand	175-178	4	18
β-strand	181	1	18
α-helix	186	1
β-strand	187	1	19
β-strand	191-192	2	20
β-strand	198-202	5	20
β-strand	203	1	19
β-strand	208-212	5	20
β-strand	218-222	5	20
β-strand	229-234	6	21
β-strand	240-245	6	21
β-strand	250-254	5	21
β-strand	259-264	6	21
α-helix	272	1
β-strand	273-278	6	22
β-strand	284-289	6	22
β-strand	294-298	5	22
β-strand	304-308	5	22
β-strand	315-320	6	15
β-strand	327-331	5	15
β-strand	336-339	4	15

Chain B4: 3 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
β-strand	47	1	23
β-strand	51	1	23
β-strand	58-63	6	24
β-strand	69-74	6	24
β-strand	78-83	6	24
β-strand	88-94	7	24
β-strand	100-105	6	25
β-strand	111-116	6	25
β-strand	121-125	5	25
β-strand	134-139	6	25
β-strand	146-151	6	26
β-strand	156-161	6	26
β-strand	165	1	26
β-strand	168-170	3	26
β-strand	176	1	26
β-strand	190-192	3	27
β-strand	198-201	4	27
β-strand	209-212	4	27
β-strand	217-221	5	27
β-strand	229-234	6	28
β-strand	240-245	6	28
β-strand	250-254	5	28
β-strand	259-264	6	28
α-helix	272	1
β-strand	273-278	6	29
β-strand	284-289	6	29
β-strand	294-298	5	29
β-strand	304-307	4	29
β-strand	315-320	6	23
β-strand	327-331	5	23
β-strand	336-339	4	23

Chain B5: 5 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	30
β-strand	58-63	6	31
β-strand	70-74	5	31
β-strand	78-82	5	31
β-strand	89-94	6	31
β-strand	100-105	6	32
β-strand	111-116	6	32
β-strand	121-125	5	32
β-strand	134-139	6	32
α-helix	145	1
β-strand	146-151	6	33
β-strand	156-161	6	33
β-strand	165-170	6	33
β-strand	176-181	6	33
β-strand	187-192	6	34
β-strand	198-203	6	34
β-strand	209-212	4	34
β-strand	218-221	4	34
β-strand	229-234	6	35
β-strand	240-245	6	35
β-strand	250-254	5	35
β-strand	259-264	6	35
β-strand	273-278	6	36
β-strand	284-289	6	36
β-strand	294-298	5	36
β-strand	303-307	5	36
α-helix	308-310	3
β-strand	315-320	6	30
β-strand	327-331	5	30
β-strand	336-339	4	30

Chain G1: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	2-7	6
α-helix	10-24	15
α-helix	30-43	14

Chain G2: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	8-23	16
α-helix	30-43	14

Chain G3: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	2-4	3
α-helix	8-23	16
α-helix	30-43	14
α-helix	53-55	3

7 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B1, B2, B3, B4, B5	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G1, G2, G3, G4, G5	protein	71	Homo sapiens	P59768 (AlphaFold model)
BTB/POZ domain-containing protein KCTD5	K1, K2, K3, K4, K5	protein	234	Homo sapiens	Q9NXV2 (AlphaFold model)

Sequence of entity 1 (B1, B2, B3, B4, B5), FASTA

>8U7Z_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B1, B2, B3, B4, B5)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (G1, G2, G3, G4, G5), FASTA

>8U7Z_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G1, G2, G3, G4, G5)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFSAIL

Sequence of entity 3 (K1, K2, K3, K4, K5), FASTA

>8U7Z_3 BTB/POZ domain-containing protein KCTD5 (chains K1, K2, K3, K4, K5)
MAENHCELLSPARGGIGAGLGGGLCRRCSAGLGALAQRPGSVSKWVRLNVGGTYFLTTRQ
TLCRDPKSFLYRLCQADPDLDSDKDETGAYLIDRDPTYFGPVLNYLRHGKLVINKDLAEE
GVLEEAEFYNITSLIKLVKDKIRERDSKTSQVPVKHVYRVLQCQEEELTQMVSTMSDGWK
FEQLVSIGSSYNYGNEDQAEFLCVVSKELHNTPYGTASEPSEKAKILQERGSRM

Primary citation

Structure and dynamics of a pentameric KCTD5/CUL3/G beta gamma E3 ubiquitin ligase complex. Nguyen, D.M., Rath, D.H., Devost, D. et al. Proc Natl Acad Sci U S A (2024) 121:e2315018121-e2315018121. DOI 10.1073/pnas.2315018121 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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