8UQO: PLCb3-Gbg-Gaq complex on membranes

PLCb3-Gbg-Gaq complex on membranes. Determined by electron microscopy at 3.37 Å resolution. Released 6 Dec 2023.

Method: Electron microscopy
Resolution: 3.37 Å
Organism: Homo sapiens
Chains: 6
Atoms: 13,959
Mol. weight: 272.26 kDa
Ligands: GDP, CA, MG, ALF
Released: 6 Dec 2023

Explore 8UQO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8UQO contains 67 α-helices and 116 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	39-45	7	21
α-helix	53-62	10
α-helix	69-72	4
α-helix	73-75	3
α-helix	76-97	22
α-helix	104-107	4
α-helix	110-114	5
α-helix	126-132	7
α-helix	139-145	7
α-helix	148-150	3
α-helix	164-167	4
α-helix	176-179	4
β-strand	189-195	7	21
β-strand	200-206	7	21
α-helix	216-219	4
β-strand	225-231	7	21
β-strand	238	1	22
β-strand	246	1	22
α-helix	247-258	12
α-helix	263-265	3
β-strand	268-274	7	21
α-helix	278-281	4
α-helix	302-314	13
β-strand	325-327	3	21
α-helix	334-348	15

Chain B: 2 helices, 36 β-strands

Element	Residues	Length	Sheet
α-helix	7-24	18
α-helix	30-33	4
β-strand	47-51	5	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	88-94	7	2
β-strand	100	1	3
β-strand	104-105	2	4
β-strand	111-114	4	4
β-strand	116	1	3
β-strand	121-125	5	4
β-strand	135-139	5	4
β-strand	146	1	5
β-strand	151	1	6
β-strand	156-157	2	6
β-strand	161	1	5
β-strand	169-170	2	6
β-strand	175-176	2	6
β-strand	190-191	2	7
β-strand	198-201	4	7
β-strand	208-212	5	7
β-strand	220-222	3	7
β-strand	229-234	6	8
β-strand	240	1	9
β-strand	241-245	5	8
β-strand	250-252	3	8
β-strand	254	1	9
β-strand	262-264	3	8
β-strand	273-277	5	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	304-307	4	10
β-strand	315	1	11
β-strand	320	1	1
β-strand	327-330	4	1
β-strand	331	1	11
β-strand	336-339	4	1

Chain C: 6 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	8-11	4
α-helix	13-23	11
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	47-48	2	12
β-strand	58	1	13
β-strand	62	1	14
β-strand	69-72	4	14
β-strand	74	1	13
β-strand	78-83	6	14
β-strand	89-94	6	14
β-strand	123-124	2	15
β-strand	135-137	3	15
β-strand	146-151	6	16
β-strand	157-161	5	16
β-strand	166-168	3	16
β-strand	178-180	3	16
β-strand	187-192	6	17
β-strand	199-203	5	17
β-strand	208-210	3	17
β-strand	220-222	3	17
β-strand	232-234	3	18
β-strand	241-243	3	18
β-strand	252-254	3	18
β-strand	259-262	4	18
α-helix	272-273	2
β-strand	276-277	2	19
β-strand	284-287	4	19
β-strand	296-298	3	19
β-strand	304-306	3	19
β-strand	315	1	20
β-strand	320	1	12
β-strand	327-330	4	12
β-strand	331	1	20
β-strand	336-339	4	12

Chain D: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-18	10
α-helix	30-32	3
α-helix	34-42	9

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	10-19	10
α-helix	20-22	3
α-helix	30-41	12
α-helix	53-55	3

Chain Q: 35 helices, 42 β-strands

Element	Residues	Length	Sheet
β-strand	26-30	5	23
β-strand	39-45	7	23
β-strand	51-55	5	23
β-strand	61-65	5	23
α-helix	66-68	3
β-strand	69-74	6	24
α-helix	75-77	3
α-helix	84-90	7
β-strand	103-108	6	24
β-strand	116	1	24
β-strand	119-121	3	24
β-strand	122	1	23
α-helix	128-131	4
α-helix	136-139	4
α-helix	142-145	4
α-helix	158-162	5
β-strand	170-171	2	25
α-helix	172-175	4
α-helix	186-192	7
β-strand	202-203	2	25
α-helix	205-207	3
α-helix	210-216	7
β-strand	241-242	2	26
α-helix	243-252	10
α-helix	265-268	4
α-helix	276-279	4
α-helix	283-286	4
β-strand	290-291	2	26
α-helix	294-297	4
β-strand	306	1	27
α-helix	323-325	3
β-strand	326-328	3	28
β-strand	336	1	29
β-strand	345	1	29
α-helix	348-354	7
β-strand	360-363	4	30
β-strand	365-366	2	31
β-strand	376-377	2	31
β-strand	387-388	2	31
α-helix	389-399	11
β-strand	408-412	5	30
α-helix	419-432	14
α-helix	434-436	3
β-strand	437	1	30
α-helix	441-442	2
β-strand	463-466	4	30
α-helix	584-586	3
α-helix	591-593	3
β-strand	598-599	2	32
α-helix	606-609	4
β-strand	616-621	6	32
α-helix	622-625	4
α-helix	635-639	5
β-strand	644-648	5	32
α-helix	661-663	3
β-strand	671-672	2	32
α-helix	681-685	5
α-helix	692-695	4
β-strand	698-700	3	28
α-helix	701-702	2
α-helix	703-705	3
β-strand	726-736	11	33
β-strand	747-752	6	34
β-strand	761-763	3	34
α-helix	765-767	3
β-strand	775	1	33
α-helix	779-780	2
β-strand	781-786	6	33
β-strand	793-799	7	34
β-strand	805-812	8	34
β-strand	819-821	3	33
β-strand	822	1	27
β-strand	826	1	34
β-strand	832	1	34
β-strand	837-842	6	33
β-strand	845-846	2	33
α-helix	862-871	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B, C	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein subunit gamma	D, G	protein	73	Homo sapiens	P59768 (AlphaFold model)
Guanine nucleotide-binding protein G(q) subunit alpha	A	protein	355	Homo sapiens	P50148 (AlphaFold model)
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3	Q	protein	1234	Homo sapiens	Q01970 (AlphaFold model)

Sequence of entity 1 (B, C), FASTA

>8UQO_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B, C)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (D, G), FASTA

>8UQO_2 Guanine nucleotide-binding protein subunit gamma (chains D, G)
GPMASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASE
NPFREKKFFCAIL

Sequence of entity 3 (A), FASTA

>8UQO_3 Guanine nucleotide-binding protein G(q) subunit alpha (chains A)
GPMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHG
SGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAF
ENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRV
PTTGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDN
ENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQA
AREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 4 (Q), FASTA

>8UQO_4 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (chains Q)
GPAMDPEFMALQLEPPTVVETLRRGSKFIKWDEETSSRNLVTLRVDPNGFFLYWTGPNME
VDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDARLEEKLMTVVSGPDPVNTVFLNF
MAVQDDTAKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFS
ADKKRVETALESCGLKFNRSESIRPDEFSLEIFERFLNKLCLRPDIDKILLEIGAKGKPY
LTLEQLMDFINQKQRDPRLNEVLYPPLRPSQARLLIEKYEPNQQFLERDQMSMEGFSRYL
GGEENGILPLEALDLSTDMTQPLSAYFINSSHNTYLTAGQLAGTSSVEMYRQALLWGCRC
VELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAETAFKTSPYPVILSFENHVDSAK
QQAKMAEYCRSIFGDALLIEPLDKYPLAPGVPLPSPQDLMGRILVKNKKRHRPSAGGPDS
AGRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKPSLEPQKSLGD
EGLNRGPYVLGPADREDEEEDEEEEEQTDPKKPTTDEGTASSEVNATEEMSTLVNYIEPV
KFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYM
PQLFWNVGCQLVALNFQTLDVAMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIV
DGIVANALRVKVISGQFLSDRKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEP
FDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLI
YTEASDYIPDDHQDYAEALINPIKHVSLMDQRARQLAALIGESEAQAGQETCQDTQSQQL
GSQPSSNPTPSPLDASPRRPPGPTTSPASTSLSSPGQRDDLIASILSEVAPTPLDELRGH
KALVKLRSRQERDLRELRKKHQRKAVTLTRRLLDGLAQAQAEGRCRLRPGALGGAADVED
TKEGEDEAKRYQEFQNRQVQSLLELREAQVDAEAQRRLEHLRQALQRLREVVLDANTTQF
KRLKEMNEREKKELQKILDRKRHNSISEAKMRDKHKKEAELTEINRRHITESVNSIRRLE
EAQKQRHDRLVAGQQQVLQQLAEEEPKLLAQLAQECQEQRARLPQEIRRSLLGEMPEGLG
DGPLVACASNGHAPGSSGHLSGADSESQEENTQL

Ligands and cofactors

ID	Name	Formula	Copies
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1
CA	Calcium ion	Ca	1
MG	Magnesium ion	Mg	1
ALF	Tetrafluoroaluminate ion	Al F4	1

Primary citation

The mechanism of G alpha q regulation of PLC beta 3 -catalyzed PIP2 hydrolysis. Falzone, M.E., MacKinnon, R. Proc Natl Acad Sci U S A (2023) 120:e2315011120-e2315011120. DOI 10.1073/pnas.2315011120 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

Browse structure collections

About this viewer

MolViewer shows 8UQO directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.