8UTT: Kinesin-like protein KIF1A

KIF1A[1-393] P305L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule. Determined by electron microscopy at 3.1 Å resolution. Released 12 Jun 2024.

Method: Electron microscopy
Resolution: 3.1 Å
Organisms: Homo sapiens, Sus scrofa
Chains: 7
Atoms: 23,744
Mol. weight: 354.5 kDa
Ligands: GTP, TA1, GDP, ANP
Released: 12 Jun 2024

Explore 8UTT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8UTT contains 154 α-helices and 130 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	16
α-helix	10-27	18
α-helix	38-42	5
β-strand	53-55	3	17
β-strand	61-63	3	17
β-strand	65-68	4	16
β-strand	69	1	18
α-helix	73-79	7
β-strand	93	1	18
α-helix	103-104	2
α-helix	105-109	5
α-helix	115-128	14
β-strand	132-140	9	16
α-helix	144-160	17
β-strand	165-171	7	16
α-helix	172-174	3
α-helix	183-196	14
β-strand	200-204	5	16
α-helix	206-215	10
α-helix	224-239	16
α-helix	240-242	3
β-strand	248	1	19
α-helix	252-258	7
β-strand	269-272	4	19
α-helix	278-281	4
α-helix	288-295	8
α-helix	298-300	3
α-helix	307-309	3
β-strand	312-321	10	19
α-helix	325-337	13
β-strand	343	1	19
β-strand	351-356	6	19
α-helix	359-361	3
α-helix	369-370	2
β-strand	373-381	9	19
α-helix	384-399	16
α-helix	405-409	5
α-helix	416-434	19
α-helix	438-439	2

Chain B: 25 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	20
α-helix	10-28	19
β-strand	30	1	21
β-strand	36	1	21
α-helix	47-49	3
β-strand	51-54	4	22
β-strand	58-61	4	22
β-strand	63-66	4	20
α-helix	70-77	8
α-helix	82-84	3
α-helix	87-89	3
β-strand	90-91	2	20
α-helix	101-102	2
α-helix	103-107	5
α-helix	109-126	18
β-strand	130-138	9	20
α-helix	143-158	16
β-strand	163-169	7	20
α-helix	170-172	3
α-helix	181-195	15
β-strand	199-202	4	20
α-helix	204-209	6
α-helix	210-214	5
α-helix	222-236	15
α-helix	238-240	3
β-strand	244-246	3	23
α-helix	250-257	8
β-strand	265-266	2	20
β-strand	267-271	5	23
α-helix	283-284	2
α-helix	286-293	8
α-helix	305-307	3
β-strand	310-319	10	23
α-helix	323-336	14
α-helix	338-340	3
β-strand	341	1	23
β-strand	349-354	6	23
β-strand	363-371	9	23
α-helix	372-374	3
α-helix	375-389	15
α-helix	395-399	5
α-helix	405-426	22

Chain E: 25 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	24
α-helix	10-28	19
α-helix	37-42	6
β-strand	53-55	3	25
β-strand	61-63	3	25
β-strand	65-68	4	24
β-strand	69	1	26
α-helix	72-79	8
β-strand	93	1	26
α-helix	104-106	3
α-helix	115-128	14
β-strand	132-140	9	24
α-helix	144	1
α-helix	145-149	5
α-helix	150-160	11
β-strand	165-171	7	24
α-helix	172-174	3
α-helix	183-196	14
β-strand	200-204	5	24
α-helix	206-212	7
α-helix	213-217	5
α-helix	224-243	20
β-strand	248	1	27
α-helix	252-259	8
β-strand	269-272	4	27
α-helix	278-281	4
α-helix	288-295	8
α-helix	298-300	3
α-helix	307-309	3
β-strand	312-321	10	27
α-helix	325-337	13
β-strand	343	1	27
β-strand	352-356	5	27
α-helix	359-361	3
α-helix	369-370	2
β-strand	373-381	9	27
α-helix	384-399	16
α-helix	405-409	5
α-helix	415-435	21
α-helix	438-440	3

Chain I: 25 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	3-5	3	28
β-strand	6-9	4	29
α-helix	10-28	19
β-strand	30	1	30
β-strand	36	1	30
α-helix	42-44	3
α-helix	47-49	3
β-strand	51-53	3	31
β-strand	59-61	3	31
β-strand	63-66	4	29
α-helix	70-77	8
α-helix	87-89	3
β-strand	90-91	2	29
α-helix	101-102	2
α-helix	103-107	5
α-helix	109-125	17
β-strand	130-133	4	28
β-strand	136-138	3	29
α-helix	142-158	17
β-strand	163-164	2	28
β-strand	167-170	4	29
α-helix	171-172	2
α-helix	181-195	15
β-strand	199-203	5	29
α-helix	204-209	6
α-helix	210-214	5
α-helix	222-236	15
α-helix	238-240	3
β-strand	244-246	3	32
α-helix	250-257	8
β-strand	265-266	2	29
β-strand	267-271	5	32
α-helix	286-293	8
α-helix	296-298	3
α-helix	305-307	3
β-strand	310-319	10	32
α-helix	323-336	14
α-helix	338-340	3
β-strand	341	1	32
β-strand	349-354	6	32
β-strand	363-371	9	32
α-helix	372-374	3
α-helix	375-389	15
α-helix	396-399	4
α-helix	405-424	20

Chain K: 15 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	5	1	1
β-strand	7-8	2	2
β-strand	9-12	4	3
α-helix	13-15	3
α-helix	17-21	5
β-strand	26	1	4
β-strand	28-31	4	5
β-strand	34-37	4	5
β-strand	48-51	4	5
β-strand	54-56	3	3
β-strand	57	1	6
β-strand	67	1	6
α-helix	70-73	4
α-helix	74-78	5
α-helix	79-87	9
β-strand	90-96	7	2
β-strand	98	1	7
α-helix	103-107	5
β-strand	109	1	8
β-strand	116	1	8
α-helix	118-133	16
β-strand	138-149	12	2
β-strand	154-156	3	2
β-strand	166	1	2
β-strand	168-171	4	9
β-strand	175-178	4	9
β-strand	184-186	3	2
α-helix	189-202	14
β-strand	205-206	2	10
β-strand	214-215	2	10
β-strand	218-229	12	2
β-strand	240-248	9	2
α-helix	249-251	3
β-strand	252	1	7
α-helix	253	1
α-helix	263-294	32
α-helix	310-314	5
α-helix	316-319	4
β-strand	323-329	7	2
β-strand	330-331	2	3
β-strand	334	1	4
α-helix	339-352	14
β-strand	355	1	1
α-helix	364-384	21

Chain N: 15 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	6-12	7	11
α-helix	17-21	5
β-strand	29-31	3	12
β-strand	34-37	4	12
β-strand	48-51	4	12
β-strand	54-57	4	11
α-helix	70-73	4
α-helix	74-78	5
α-helix	79-86	8
β-strand	90-96	7	11
α-helix	103-108	6
β-strand	109	1	13
β-strand	116	1	13
α-helix	118-131	14
β-strand	138-150	13	11
β-strand	153-156	4	11
β-strand	168-171	4	14
β-strand	175-178	4	14
β-strand	184-185	2	11
α-helix	189-201	13
β-strand	205-206	2	15
β-strand	214-215	2	15
β-strand	218-229	12	11
β-strand	237-248	12	11
α-helix	249-252	4
α-helix	263-295	33
α-helix	310-314	5
α-helix	316-319	4
β-strand	323-331	9	11
α-helix	338-348	11
α-helix	353	1
β-strand	354	1	12
α-helix	355	1
α-helix	364-384	21

Chain S: 25 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	33
α-helix	10-28	19
α-helix	37-42	6
β-strand	53-55	3	34
β-strand	61-63	3	34
β-strand	65-68	4	33
β-strand	69	1	35
α-helix	72-79	8
β-strand	93	1	35
α-helix	103-104	2
α-helix	105-109	5
α-helix	115-127	13
β-strand	132-138	7	33
α-helix	144	1
α-helix	145-149	5
α-helix	150-160	11
β-strand	165-172	8	33
α-helix	173-174	2
α-helix	183-196	14
β-strand	200-205	6	33
α-helix	206-215	10
α-helix	224-239	16
α-helix	240-242	3
β-strand	248	1	33
α-helix	252-259	8
β-strand	269-272	4	33
α-helix	278-281	4
α-helix	288-296	9
α-helix	307-309	3
β-strand	312-321	10	33
α-helix	325-336	12
β-strand	343	1	33
β-strand	351-356	6	33
α-helix	359-361	3
α-helix	368-370	3
β-strand	373-381	9	33
α-helix	384-399	16
α-helix	405-410	6
α-helix	415-435	21
α-helix	438-439	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Kinesin-like protein KIF1A	K, N	protein	438	Homo sapiens	Q12756 (AlphaFold model)
Tubulin alpha-1B chain	A, E, S	protein	451	Sus scrofa	Q2XVP4 (AlphaFold model)
Tubulin beta-2B chain	B, I	protein	445	Sus scrofa	A0A8D1UIR5 (AlphaFold model)

Sequence of entity 1 (K, N), FASTA

>8UTT_1 Kinesin-like protein KIF1A (chains K, N)
MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHT
SPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIP
QLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVED
LSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEK
VSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKK
TDFILYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVI
NEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDGAGVKQLEDKVEELASKNYHLENEVAR
LKKLVEFTSAWSHPQFEK

Sequence of entity 2 (A, E, S), FASTA

>8UTT_2 Tubulin alpha-1B chain (chains A, E, S)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Sequence of entity 3 (B, I), FASTA

>8UTT_3 Tubulin beta-2B chain (chains B, I)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMM
AACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEEGEDEA

Ligands and cofactors

ID	Name	Formula	Copies
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	3
TA1	Taxol	C47 H51 N O14	2
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	2
ANP	Phosphoaminophosphonic acid-adenylate ester	C10 H17 N6 O12 P3	2
MG	Magnesium ion	Mg	4

Primary citation

Cryo-EM unveils kinesin KIF1A's processivity mechanism and the impact of its pathogenic variant P305L. Benoit, M.P.M.H., Rao, L., Asenjo, A.B. et al. Nat Commun (2024) 15:5530-5530. DOI 10.1038/s41467-024-48720-4 · PubMed

Other PDB entries of the same protein (UniProt Q12756 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4EJQ 1.89 Å, Crystal structure of KIF1A C-CC1-FHA
4EGX 2.51 Å, Crystal structure of KIF1A CC1-FHA tandem
8UTS 2.7 Å, KIF1A[1-393] APO in complex with a microtubule
9YA5 2.95 Å, KIF1A R350G bound to microtubules in two-heads-bound state with AMP-PNP
8UTU 3.0 Å, KIF1A[1-393] P305L mutant AMP-PNP bound one and two heads bound states merged, in…
8UTV 3.0 Å, KIF1A[1-393] P305L mutant ADP bound in complex with a microtubule
8UTN 3.1 Å, KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule (class…
8UTQ 3.1 Å, KIF1A[1-393] AMP-PNP bound one-head-bound state in complex with a microtubule - class…
9YAI 3.12 Å, KIF1A R350W bound to microtubules in two-heads-bound state with AMP-PNP
8UTO 3.2 Å, KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule - class…
8UTP 3.2 Å, KIF1A[1-393] - AMP-PNP two-heads-bound state in complex with a microtubule - class T3L1
9YAB 3.21 Å, KIF1A R350W bound to microtubules in the apo state

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