8WXE: Vgamma5Vdelta1 EH TCR-CD3 complex
Vgamma5Vdelta1 EH TCR-CD3 complex. Determined by electron microscopy at 4.0 Å resolution. Released 8 May 2024.
- Method
- Electron microscopy
- Resolution
- 4.0 Å
- Organism
- Homo sapiens
- Chains
- 8
- Atoms
- 4,388
- Mol. weight
- 201.82 kDa
- Ligands
- CLR
- Released
- 8 May 2024
Explore 8WXE in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8WXE contains 10 α-helices and 36 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains a and b: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 28-53 | 26 | |
Chain d: 1 helix, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 28 | 1 | 1 |
| β-strand | 33-36 | 4 | 1 |
| β-strand | 41-46 | 6 | 2 |
| β-strand | 57-61 | 5 | 1 |
| β-strand | 68-73 | 6 | 2 |
| β-strand | 84-90 | 7 | 2 |
| β-strand | 96-98 | 3 | 3 |
| α-helix | 101-124 | 24 | |
Chain e: 2 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 34-36 | 3 | |
| β-strand | 37-40 | 4 | 4 |
| β-strand | 45-48 | 4 | 4 |
| β-strand | 58-61 | 4 | 2 |
| β-strand | 65 | 1 | 2 |
| β-strand | 76-77 | 2 | 4 |
| β-strand | 81-84 | 4 | 4 |
| β-strand | 95-99 | 5 | 2 |
| β-strand | 110-115 | 6 | 2 |
| β-strand | 122-124 | 3 | 3 |
| α-helix | 127-151 | 25 | |
Chain f: 2 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 34-37 | 4 | |
| β-strand | 38 | 1 | 5 |
| β-strand | 41 | 1 | 6 |
| β-strand | 44 | 1 | 6 |
| β-strand | 45-47 | 3 | 5 |
| β-strand | 60 | 1 | 7 |
| β-strand | 75-77 | 3 | 5 |
| β-strand | 82-84 | 3 | 5 |
| β-strand | 94 | 1 | 8 |
| β-strand | 97 | 1 | 7 |
| β-strand | 114 | 1 | 8 |
| β-strand | 122-123 | 2 | 9 |
| α-helix | 129-152 | 24 | |
Chain g: 1 helix, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 31-34 | 4 | 10 |
| β-strand | 42-46 | 5 | 10 |
| β-strand | 55-57 | 3 | 11 |
| β-strand | 60-63 | 4 | 11 |
| β-strand | 73-75 | 3 | 10 |
| β-strand | 84-85 | 2 | 12 |
| β-strand | 97-98 | 2 | 12 |
| β-strand | 100 | 1 | 8 |
| β-strand | 108-109 | 2 | 9 |
| α-helix | 112-139 | 28 | |
Chain m: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 259-287 | 29 | |
Chain n: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 253-287 | 35 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| T-cell surface glycoprotein CD3 zeta chain | a, b | protein | 195 | Homo sapiens | P20963 (AlphaFold model) |
| T-cell surface glycoprotein CD3 delta chain | d | protein | 171 | Homo sapiens | P04234 (AlphaFold model) |
| T-cell surface glycoprotein CD3 epsilon chain | e, f | protein | 207 | Homo sapiens | P07766 (AlphaFold model) |
| T-cell surface glycoprotein CD3 gamma chain | g | protein | 182 | Homo sapiens | P09693 (AlphaFold model) |
| Signal peptide,flag tag,T cell receptor delta variable 1,T cell receptor delta constant | m | protein | 307 | Homo sapiens | A0A1B0GX56, B7Z8K6 |
| Signal peptide,flag tag,T cell receptor gamma variable 5,T cell receptor gamma constant 1 | n | protein | 331 | Homo sapiens | A0A0B4J1U4 |
Sequence of entity 1 (a, b), FASTA
>8WXE_1 T-cell surface glycoprotein CD3 zeta chain (chains a, b)
MKWKALFTAAILQAQLPITEAQSFGLLDPKLCYLLDGILFIYGVILTALFLRVKFSRSAD
APAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMA
EAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPRAAAWSHPQFEKGGGSG
GGSGGSAWSHPQFEK
Sequence of entity 2 (d), FASTA
>8WXE_2 T-cell surface glycoprotein CD3 delta chain (chains d)
MEHSTFLSGLVLATLLSQVSPFKIPIEELEDRVFVNCNTSITWVEGTVGTLLSDITRLDL
GKRILDPRGIYRCNGTDIYKDKESTVQVHYRMCQSCVELDPATVAGIIVTDVIATLLLAL
GVFCFAGHETGRLSGAADTQALLRNDQVYQPLRDRDDAQYSHLGGNWARNK
Sequence of entity 3 (e, f), FASTA
>8WXE_3 T-cell surface glycoprotein CD3 epsilon chain (chains e, f)
MQSGTHWRVLGLCLLSVGVWGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQ
HNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCE
NCMEMDVMSVATIVIVDICITGGLLLLVYYWSKNRKAKAKPVTRGAGAGGRQRGQNKERP
PPVPNPDYEPIRKGQRDLYSGLNQRRI
Sequence of entity 4 (g), FASTA
>8WXE_4 T-cell surface glycoprotein CD3 gamma chain (chains g)
MEQGKGLAVLILAIILLQGTLAQSIKGNHLVKVYDYQEDGSVLLTCDAEAKNITWFKDGK
MIGFLTEDKKKWNLGSNAKDPRGMYQCKGSQNKSKPLQVYYRMCQNCIELNAATISGFLF
AEIVSIFVLAVGVYFIAGQDGVRQSRASDKQTLLPNDQLYQPLKDREDDQYSHLQGNQLR
RN
Sequence of entity 5 (m), FASTA
>8WXE_5 Signal peptide,flag tag,T cell receptor delta variable 1,T cell receptor delta constant (chains m)
MDMRVPAQLLGLLLLWLSGARCMDYKDDDDKGGSETGAQKVTQAQSSVSMPVRKAVTLNC
LYETSWWSYYIFWYKQLPSKEMIFLIRQGSDEQNAKSGRYSVNFKKAAKSVALTISALQL
EDSAKYFCALGDPGGLNTDKLIFGKGTRVTVEPRSQPHTKPSVFVMKNGTNVACLVKEFY
PKDIRINLVSSKKITEFDPAIVISPSGKYNAVKLGKYEDSNSVTCSVQHDNKTVHSTDFE
VKTDSTDHVKPKETENTKQPSKSCHKPKAIVHTEKVNMMSLTVLGLRMLFAKTVAVNFLL
TAKLFFL
Sequence of entity 6 (n), FASTA
>8WXE_6 Signal peptide,flag tag,T cell receptor gamma variable 5,T cell receptor gamma constant 1 (chains n)
MDMRVPAQLLGLLLLWLSGARCMDYKDDDDKGGSETGSSNLEGGTKSVTRPTRSSAEITC
DLTVINAFYIHWYLHQEGKAPQRLLYYDVSNSKDVLESGLSPGKYETHTPRRWSWILILH
NLIENDSGVYYCATWDRGNPKTHYYKKLFGSGTTLVVTDKQLDADVSPKPTIFLPSIAET
KLQKAGTYLCLLEKFFPDVIKIHWQEKKSNTILGSQEGNTMKTNDTYMKFSWLTVPEKSL
DKEHRCIVRHENNKNGVDQEIIFPPIKTDVITMDPKDNCSKDANDTLLLQLTNTSAYYMY
LLLLLKSVVYFAIITCCLLRRTAFCCNGEKS
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CLR | Cholesterol | C27 H46 O | 1 |
Primary citation
Structures of human gamma delta T cell receptor-CD3 complex. Xin, W., Huang, B., Chi, X. et al. Nature (2024) 630:222-229. DOI 10.1038/s41586-024-07439-4 · PubMed
Other PDB entries of the same protein (UniProt P20963 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 2OQ1 1.9 Å, Tandem SH2 domains of ZAP-70 with 19-mer zeta1 peptide
- 3IK5 2.05 Å, SIVmac239 Nef in complex with TCR zeta ITAM 1 polypeptide (A63-R80)
- 8ES8 2.65 Å, CryoEM structure of PN45545 TCR-CD3 in complex with HLA-A2 MAGEA4 (230-239)
- 4XZ1 2.8 Å, ZAP-70-tSH2:Compound-B adduct
- 1YGR 2.9 Å, Crystal structure of the tandem phosphatase domain of RPTP CD45
- 7FJE 3.0 Å, Cryo-EM structure of a membrane protein(LL)
- 9CI8 3.01 Å, T cell receptor complex
- 8ES7 3.04 Å, CryoEM structure of PN45545 TCR-CD3 complex
- 7PHR 3.08 Å, Structure of a fully assembled T-cell receptor engaging a tumor-associated peptide-MHC I
- 9JY1 3.08 Å, delta epsilon/delta epsilon Fab-TCR tetramer
- 7FJF 3.1 Å, Cryo-EM structure of a membrane protein(CS)
- 8TW6 3.1 Å, TCR in nanodisc ND-II
Browse structure collections
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