8XIQ: L796778-SSTR3 G protein complex

Structure of L796778-SSTR3 G protein complex. Determined by electron microscopy at 2.71 Å resolution. Released 3 Jul 2024.

Method: Electron microscopy
Resolution: 2.71 Å
Organisms: Homo sapiens, Lama glama, synthetic construct
Chains: 6
Atoms: 8,169
Mol. weight: 147.65 kDa
Released: 3 Jul 2024

Explore 8XIQ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8XIQ contains 34 α-helices and 46 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	43-70	28
α-helix	78-94	17
α-helix	97-105	9
α-helix	113-146	34
α-helix	148-151	4
α-helix	157-181	25
β-strand	183	1	1
β-strand	194	1	1
α-helix	199-211	13
α-helix	212-216	5
α-helix	217-240	24
α-helix	243-245	3
α-helix	248-282	35
α-helix	284-287	4
α-helix	289-310	22
α-helix	311-315	5
α-helix	318-323	6
α-helix	324-326	3

Chain B: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	33-40	8	2
α-helix	42-44	3
α-helix	46-50	5
β-strand	184-191	8	2
β-strand	194-201	8	2
α-helix	211-217	7
β-strand	220-226	7	2
α-helix	230-244	15
β-strand	253-259	7	2
α-helix	261-270	10
α-helix	275-277	3
α-helix	280-284	5
α-helix	299-317	19
β-strand	326-330	5	2
α-helix	338-358	21

Chain C: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	12-30	19
α-helix	35-38	4
α-helix	43-44	2
α-helix	50-51	2
β-strand	52-57	6	3
β-strand	63-68	6	4
β-strand	74-79	6	4
β-strand	83-88	6	4
β-strand	94-99	6	4
β-strand	105-110	6	5
β-strand	116-121	6	5
β-strand	125-130	6	5
β-strand	139-145	7	5
β-strand	151-156	6	6
β-strand	161-166	6	6
β-strand	171-175	5	6
β-strand	181-185	5	6
β-strand	194-197	4	7
β-strand	203-207	5	7
β-strand	213-217	5	7
β-strand	223-227	5	7
β-strand	234-239	6	8
β-strand	245-250	6	8
β-strand	255-259	5	8
β-strand	264-269	6	8
β-strand	278-283	6	9
β-strand	289-294	6	9
β-strand	299-303	5	9
β-strand	309-313	5	9
β-strand	320-325	6	3
β-strand	332-336	5	3
β-strand	340-344	5	3

Chain D: 2 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	5-9	5	10
β-strand	19-27	9	10
α-helix	31-33	3
β-strand	36-41	6	11
β-strand	47-53	7	11
β-strand	60-62	3	11
β-strand	70-75	6	10
β-strand	80-86	7	10
α-helix	90-92	3
β-strand	95-100	6	11
β-strand	120	1	11
β-strand	124	1	11

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-23	15
α-helix	30-43	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Somatostatin receptor type 3	A	protein	369	Homo sapiens	P32745 (AlphaFold model)
G-alpha i	B	protein	361	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	369	Homo sapiens	P62873 (AlphaFold model)
nanobody Nb35	D	protein	156	Lama glama
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nit-FC0-nle-A1D5B	F	protein	4	synthetic construct

Sequence of entity 1 (A), FASTA

>8XIQ_1 Somatostatin receptor type 3 (chains A)
MDMLHPSSVSTTSEPENASSAWPPDATLGNVSAGPSPAGLAVSGVLIPLVYLVVCVVGLL
GNSLVIYVVLRHTASPSVTNVYILNLALADELFMLGLPFLAAQNALSYWPFGSLMCRLVM
AVDGINQFTSIFCLTVMSVDRYLAVVHPTRSARWRTAPVARTVSAAVWVASAVVVLPVVV
FSGVPRGMSTCHMQWPEPAAAWRAGFIIYTAALGFFGPLLVICLCYLLIVVKVRSAGRRV
WAPSCQRRRRSERRVTRMVVAVVALFVLCWMPFYVLNIVNVVCPLPEEPAFFGLYFLVVA
LPYANSCANPILYGFLSYRFKQGFRRVLLRPSRRVRSQEPTVGPPEKTEEEDEEEEDGEE
SREGGKGKE

Sequence of entity 2 (B), FASTA

>8XIQ_2 G-alpha i (chains B)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDVTDIIIKMNLRDCGL
F

Sequence of entity 3 (C), FASTA

>8XIQ_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAK
IYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGL
DNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQT
TTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPN
GNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVW
DALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSGVS
GWRLFKKIS

Sequence of entity 4 (D), FASTA

>8XIQ_4 nanobody Nb35 (chains D)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHH

Sequence of entity 5 (G), FASTA

>8XIQ_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (F), FASTA

>8XIQ_6 NIT-FC0-NLE-A1D5B (chains F)
XFLK

Primary citation

Selective ligand recognition and activation of somatostatin receptors SSTR1 and SSTR3. Wang, Y., Xu, Y., Wang, Y. et al. Proc Natl Acad Sci U S A (2024) 121:e2400298121-e2400298121. DOI 10.1073/pnas.2400298121 · PubMed

Other PDB entries of the same protein (UniProt P32745 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8XIR 2.52 Å, Structure of pasireotide-SSTR3 G protein complex
8ZBI 2.79 Å, Cryo-EM structure of Somatostatin receptor 3 (SSTR3) with Gi1 complex

Browse structure collections

About this viewer

MolViewer shows 8XIQ directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.