8XPU: Overall structure of the LAT1-4F2hc

Overall structure of the LAT1-4F2hc bound with JPH203. Determined by electron microscopy at 3.3 Å resolution. Released 17 Jul 2024.

Method: Electron microscopy
Resolution: 3.3 Å
Organism: Homo sapiens
Chains: 2
Atoms: 7,391
Mol. weight: 129.52 kDa
Ligands: VRW
Released: 17 Jul 2024

Explore 8XPU in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8XPU contains 45 α-helices and 25 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 21 β-strands

Element	Residues	Length	Sheet
α-helix	167-173	7
α-helix	180-206	27
α-helix	209-211	3
α-helix	219-221	3
β-strand	225-228	4	1
α-helix	231-235	5
α-helix	243-255	13
β-strand	260-262	3	1
α-helix	263-264	2
β-strand	266	1	2
β-strand	281	1	2
α-helix	288-300	13
β-strand	305-306	2	1
β-strand	307-308	2	3
α-helix	325-341	17
β-strand	346-348	3	3
α-helix	357-371	15
β-strand	377-381	5	3
α-helix	386-392	7
β-strand	400-403	4	3
α-helix	414-427	14
β-strand	432-434	3	4
α-helix	442-445	4
α-helix	451-458	8
β-strand	463-465	3	4
β-strand	466-468	3	1
α-helix	488-490	3
α-helix	510-514	5
α-helix	520-533	14
α-helix	535-539	5
β-strand	541-544	4	5
β-strand	551-557	7	5
β-strand	563-569	7	5
β-strand	575-576	2	6
α-helix	586-588	3
β-strand	593-594	2	7
β-strand	599	1	5
β-strand	610-611	2	7
β-strand	616-617	2	6
β-strand	621-627	7	5

Chain B: 26 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	50	1	8
α-helix	52-63	12
α-helix	73-79	7
α-helix	82-109	28
α-helix	118-123	6
α-helix	127-135	9
α-helix	136-140	5
α-helix	141-156	16
α-helix	165-167	3
α-helix	168-188	21
α-helix	191-219	29
α-helix	244-248	5
α-helix	256-258	3
α-helix	260-262	3
β-strand	267	1	8
α-helix	270-299	30
α-helix	302-306	5
α-helix	314-318	5
α-helix	326-353	28
α-helix	354-356	3
β-strand	367	1	9
β-strand	372	1	9
α-helix	374-385	12
α-helix	386-389	4
α-helix	401-421	21
α-helix	435-453	19
α-helix	455-466	12
α-helix	469-473	5
α-helix	486-500	15
α-helix	504-506	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform 5 of Amino acid transporter heavy chain SLC3A2	A	protein	647	Homo sapiens	P08195 (AlphaFold model)
Large neutral amino acids transporter small subunit 1	B	protein	527	Homo sapiens	Q01650 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8XPU_1 Isoform 5 of Amino acid transporter heavy chain SLC3A2 (chains A)
MAHHHHHHHHHHSGRELQPPEASIAVVSIPRQLPGSHSEAGVQGLSAGDDSETGSDCVTQ
AGLQLLASSDPPALASKNAEVTVETGFHHVSQADIEFLTSIDPTASASGSAGITGTMSQD
TEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVAEDEAEAAAAAKFTGLS
KEELLKVAGSPGWVRTRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPAQKWWHTGALY
RIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFG
SKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGF
QVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYL
SDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPV
FSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRR
LSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPA
SASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAALE

Sequence of entity 2 (B), FASTA

>8XPU_2 Large neutral amino acids transporter small subunit 1 (chains B)
MADYKDDDDKSGPDEVDASGRAGAGPKRRALAAPAAEEKEEAREKMLAAKSADGSAPAGE
GEGVTLQRNITLLNGVAIIVGTIIGSGIFVTPTGVLKEAGSPGLALVVWAACGVFSIVGA
LCYAELGTTISKSGGDYAYMLEVYGSLPAFLKLWIELLIIRPSSQYIVALVFATYLLKPL
FPTCPVPEEAAKLVACLCVLLLTAVNCYSVKAATRVQDAFAAAKLLALALIILLGFVQIG
KGDVSNLDPNFSFEGTKLDVGNIVLALYSGLFAYGGWNYLNFVTEEMINPYRNLPLAIII
SLPIVTLVYVLTNLAYFTTLSTEQMLSSEAVAVDFGNYHLGVMSWIIPVFVGLSCFGSVN
GSLFTSSRLFFVGSREGHLPSILSMIHPQLLTPVPSLVFTCVMTLLYAFSKDIFSVINFF
SFFNWLCVALAIIGMIWLRHRKPELERPIKVNLALPVFFILACLFLIAVSFWKTPVECGI
GFTIILSGLPVYFFGVWWKNKPKWLLQGIFSTTVLCQKLMQVVPQET

Ligands and cofactors

ID	Name	Formula	Copies
VRW	Nanvuranlat	C23 H19 Cl2 N3 O4	1

Primary citation

Structural basis for the inhibition mechanism of LAT1-4F2hc complex by JPH203. Hu, Z., Yan, R. Cell Discov (2024) 10:73-73. DOI 10.1038/s41421-024-00697-6 · PubMed

Other PDB entries of the same protein (UniProt P08195 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6S8V 1.8 Å, Structure of the high affinity Anticalin P3D11 in complex with the human CD98 heavy…
2DH2 2.1 Å, Crystal Structure of human ED-4F2hc
8G0M 2.25 Å, Structure of complex between TV6.6 and CD98hc ECD
9KJU 2.7 Å, Local refinement of the y+LAT1-4F2hc bound with Arg
2DH3 2.8 Å, Crystal Structure of human ED-4F2hc
7DF1 2.81 Å, Crystal structure of human CD98 heavy chain extracellular domain in complex with S1-F4…
7CMI 2.9 Å, The LAT2-4F2hc complex in complex with leucine
7DSK 2.9 Å, Overall structure of the LAT1-4F2hc bound with JX-075
7DSL 2.9 Å, Overall structure of the LAT1-4F2hc bound with JX-078
8YLP 2.9 Å, Overall structure of the y+LAT1-4F2hc bound with Arg
7DSN 3.1 Å, Overall structure of the LAT1-4F2hc bound with JX-119
8X0W 3.1 Å, Overall structure of the LAT1-4F2hc bound with Leu

Browse structure collections

About this viewer

MolViewer shows 8XPU directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.