8XVH: ETBR

Cryo-EM structure of ETBR bound with Endothelin1. Determined by electron microscopy at 3.26 Å resolution. Released 28 Aug 2024.

Method: Electron microscopy
Resolution: 3.26 Å
Organisms: Homo sapiens, Lama glama, Clostridium perfringens
Chains: 6
Atoms: 8,544
Mol. weight: 190.05 kDa
Released: 28 Aug 2024

Explore 8XVH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8XVH contains 31 α-helices and 54 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	10-37	28
β-strand	40-46	7	1
α-helix	53-61	9
β-strand	208-214	7	1
β-strand	217-223	7	1
α-helix	234-238	5
β-strand	243-246	4	1
β-strand	249	1	2
α-helix	256-267	12
β-strand	276-279	4	1
β-strand	281	1	3
β-strand	282	1	2
α-helix	284-293	10
α-helix	298-301	4
α-helix	305-307	3
α-helix	322-341	20
β-strand	349-350	2	1
β-strand	353	1	3
α-helix	361-380	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	88-94	7	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	120-125	6	6
β-strand	135-140	6	6
β-strand	146-151	6	7
β-strand	156-161	6	7
β-strand	166-170	5	7
β-strand	175-180	6	7
β-strand	189-192	4	8
β-strand	198-202	5	8
β-strand	207-212	6	8
β-strand	218-223	6	8
β-strand	229-232	4	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	304	1	10
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	335-339	5	4

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-43	14
α-helix	45-47	3

Chain N: 1 helix, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	11	1	12
β-strand	18-25	8	11
α-helix	29-31	3
β-strand	34-39	6	13
β-strand	46-51	6	13
β-strand	58-60	3	13
β-strand	68-73	6	11
β-strand	78-83	6	11
β-strand	92-95	4	13
β-strand	98	1	13
β-strand	122-124	3	13
β-strand	125	1	12

Chain R: 14 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	88-90	3
α-helix	98-128	31
α-helix	135-164	30
α-helix	173-175	3
α-helix	177-204	28
α-helix	216-231	16
α-helix	234-239	6
β-strand	240-243	4	14
β-strand	246-247	2	15
β-strand	250-251	2	15
β-strand	254-257	4	14
α-helix	265-281	17
α-helix	283-301	19
α-helix	312-335	24
α-helix	337-349	13
α-helix	357-375	19
α-helix	378-387	10
α-helix	391-398	8

Chain T: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-16	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	261	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	346	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	157	Lama glama
Endothelin-1	T	protein	21	Homo sapiens	P05305 (AlphaFold model)
Exo-alpha-sialidase,Endothelin receptor type B	R	protein	837	Clostridium perfringens, Homo sapiens	P24530, Q59310

Sequence of entity 1 (A), FASTA

>8XVH_1 Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
HHHHHHENLYFQGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSG
KSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTA
IIFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFP
EFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENAR
RIFNDCKDIILQMNLREYNLV

Sequence of entity 2 (B), FASTA

>8XVH_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
IGRARGFSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGH
LAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVAC
GGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETG
QQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICF
FPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNC
NVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>8XVH_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>8XVH_4 Nanobody 35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAMQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWV
RQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCA
RCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHH

Sequence of entity 5 (T), FASTA

>8XVH_5 Endothelin-1 (chains T)
CSCSSLMDKECVYFCHLDIIW

Sequence of entity 6 (R), FASTA

>8XVH_6 Exo-alpha-sialidase,Endothelin receptor type B (chains R)
MKTIIALSYIFCLVFADYKDDDDAGRAVEGAVKTEPVDLFHPGFLNSSNYRIPALFKTKE
GTLIASIDARRHGGADAPNNDIDTAVRRSEDGGKTWDEGQIIMDYPDKSSVIDTTLIQDD
ETGRIFLLVTHFPSKYGFWNAGLGSGFKNIDGKEYLCLYDSSGKEFTVRENVVYDKDSNK
TEYTTNALGDLFKNGTKIDNINSSTAPLKAKGTSYINLVYSDDDGKTWSEPQNINFQVKK
DWMKFLGIAPGRGIQIKNGEHKGRIVVPVYYTNEKGKQSSAVIYSDDSGKNWTIGESPND
NRKLENGKIINSKTLSDDAPQLTECQVVEMPNGQLKLFMRNLSGYLNIATSFDGGATWDE
TVEKDTNVLEPYCQLSVINYSQKVDGKDAVIFSNPNARSRSNGTVRIGLINQVGTYENGE
PKYEFDWKYNKLVKPGYYAYSCLTELSNGNIGLLYEGTPSEEMSYIEMNLKYLESGANKA
PAEVPKGDRTAGSPPRTISPPPCQGPIEIKETFKYINTVVSCLVFVLGIIGNSTLLRIIY
KNKCMRNGPNILIASLALGDLLHIVIDIPINVYKLLAEDWPFGAEMCKLVPFIQKASVGI
TVLSLCALSIDRYRAVASWSRIKGIGVPKWTAVEIVLIWVVSVVLAVPEAIGFDIITMDY
KGSYLRICLLHPVQKTAFMQFYKTAKDWWLFSFYFCLPLAITAFFYTLMTCEMLRKKSGM
QIALNDHLKQRREVAKTVFCLVLVFALCWLPLHLSRILKLTLYNQNDPNRCELLSFLLVL
DYIGINMASLNSCINPIALYLVSKRFKNCFKSCLCCWCQLEVLFQGPHHHHHHHHHH

Primary citation

Structural basis of antagonist selectivity in endothelin receptors. Hou, J., Liu, S., Zhang, X. et al. Cell Discov (2024) 10:79-79. DOI 10.1038/s41421-024-00705-9 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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