8XVI: ETAR

Cryo-EM structure of ETAR bound with Endothelin1. Determined by electron microscopy at 3.32 Å resolution. Released 28 Aug 2024.

Method: Electron microscopy
Resolution: 3.32 Å
Organisms: Homo sapiens, Lama glama, Acetivibrio thermocellus ATCC 27405
Chains: 6
Atoms: 8,587
Mol. weight: 173.81 kDa
Released: 28 Aug 2024

Explore 8XVI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8XVI contains 31 α-helices and 52 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	10-37	28
β-strand	41-44	4	1
β-strand	45-46	2	2
α-helix	54-59	6
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	234-237	4
β-strand	243-249	7	2
α-helix	257-267	11
β-strand	276-282	7	2
α-helix	284-292	9
α-helix	308-311	4
α-helix	322-341	20
β-strand	349-353	5	2
α-helix	363-378	16

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	3
β-strand	58-62	5	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	103-105	3	5
β-strand	111-114	4	5
β-strand	120-125	6	5
β-strand	135-140	6	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	175-181	7	6
β-strand	187-189	3	7
β-strand	198-203	6	7
β-strand	208-212	5	7
β-strand	218-222	5	7
β-strand	229-232	4	8
β-strand	241-245	5	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	275-277	3	9
β-strand	284-288	5	9
β-strand	293-298	6	9
β-strand	304-309	6	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-44	15
α-helix	45-47	3

Chain N: 1 helix, 13 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	11	1	11
β-strand	17-25	9	10
α-helix	29-31	3
β-strand	35-39	5	12
β-strand	45-51	7	12
β-strand	58-59	2	12
β-strand	61	1	13
β-strand	64	1	13
β-strand	68-73	6	10
β-strand	78-84	7	10
β-strand	92-96	5	12
β-strand	122-124	3	12
β-strand	125	1	11

Chain R: 15 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	77-107	31
α-helix	114-142	29
α-helix	146	1
α-helix	152-187	36
α-helix	200-215	16
α-helix	218-221	4
β-strand	225-227	3	14
β-strand	230-231	2	15
β-strand	234-235	2	15
β-strand	238-240	3	14
α-helix	246-253	8
α-helix	256-260	5
α-helix	261-265	5
α-helix	266-283	18
α-helix	294-331	38
α-helix	340-359	20
α-helix	360-362	3
α-helix	364-370	7
α-helix	376-381	6

Chain T: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-16	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	261	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	346	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	157	Lama glama
Endoglucanase H,Endothelin-1 receptor	R	protein	683	Acetivibrio thermocellus ATCC 27405, Homo sapiens	P16218 (AlphaFold model), P25101
Endothelin-1	T	protein	21	Homo sapiens	P05305

Sequence of entity 1 (A), FASTA

>8XVI_1 Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
HHHHHHENLYFQGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSG
KSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTA
IIFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFP
EFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENAR
RIFNDCKDIILQMNLREYNLV

Sequence of entity 2 (B), FASTA

>8XVI_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
IGRARGFSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGH
LAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVAC
GGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETG
QQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICF
FPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNC
NVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>8XVI_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>8XVI_4 Nanobody 35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAMQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWV
RQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCA
RCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHH

Sequence of entity 5 (R), FASTA

>8XVI_5 Endoglucanase H,Endothelin-1 receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDAGRAMASNYNSGLKIGAWVGTQPSESAIKSFQELQGR
KLDIVHQFINWSTDFSWVRPYADAVYNNGSILMITWEPWEYNTVDIKNGKADAYITRMAQ
DMKAYGKEIWLRPLHAANGDWYPWAIGYSSRVNTNETYIAAFRHIVDIFRANGATNVKWV
FNVNCDNVGNGTSYLGHYPGDNYVDYTSIDGYNWGTTQSWGSQWQSFDQVFSRAYQALAS
INKPIIIAEFASAEIGGNKARWITEAYNSIRTSYNKVIAAVWFHENKETDWRINSSPEAL
AAYREAIGATTHQPTNLVLPSNGSMHNYCPQQTKITSAFKYINTVISCTIFIVGMVGNAT
LLRIIYQNKCMRNGPNALIASLALGDLIYVVIDLPINVFKLLAGRWPFDHNDFGVFLCKL
FPFLQKSSVGITVLNLCALSVDRYRAVASWSRVQGIGIPLVTAIEIVSIWILSFILAIPE
AIGFVMVPFEYRGEQHKTCMLNATSKFMEFYQDVKDWWLFGFYFCMPLVCTAIFYTLMTC
EMLNRRNGSLRIALSEHLKQRREVAKTVFCLVVIFALCWFPLHLSRILKKTVYNEMDKNR
CELLSFLLLMDYIGINLATMNSCINPIALYFVSKKFKNCFQSCLCCCCYQSKSLMTSVPM
NGTSILEVLFQGPHHHHHHHHHH

Sequence of entity 6 (T), FASTA

>8XVI_6 Endothelin-1 (chains T)
CSCSSLMDKECVYFCHLDIIW

Primary citation

Structural basis of antagonist selectivity in endothelin receptors. Hou, J., Liu, S., Zhang, X. et al. Cell Discov (2024) 10:79-79. DOI 10.1038/s41421-024-00705-9 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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