8ZPS: Prolactin-releasing peptide recognition with Gi

Cryo-EM structure of prolactin-releasing peptide recognition with Gi. Determined by electron microscopy at 2.97 Å resolution. Released 25 Sept 2024.

Method: Electron microscopy
Resolution: 2.97 Å
Organism: Homo sapiens
Chains: 6
Atoms: 8,848
Mol. weight: 159.31 kDa
Released: 25 Sept 2024

Explore 8ZPS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8ZPS contains 30 α-helices and 62 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-40	7	1
α-helix	46-53	8
β-strand	184-191	8	1
β-strand	194-201	8	1
α-helix	211-215	5
β-strand	220-226	7	1
α-helix	234-244	11
α-helix	247-249	3
β-strand	254-259	6	1
α-helix	261-267	7
α-helix	299-317	19
β-strand	328-330	3	1
α-helix	338-358	21

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	12-29	18
α-helix	36-39	4
α-helix	43-44	2
β-strand	52-56	5	2
β-strand	63-68	6	3
β-strand	74-79	6	3
β-strand	83-88	6	3
β-strand	94-99	6	3
β-strand	105-110	6	4
β-strand	116-121	6	4
β-strand	126-130	5	4
β-strand	139-144	6	4
β-strand	153-156	4	5
β-strand	161-165	5	5
β-strand	171-175	5	5
β-strand	181-185	5	5
β-strand	192-197	6	6
β-strand	203-208	6	6
β-strand	212-217	6	6
β-strand	225-228	4	6
β-strand	234-239	6	7
β-strand	245-250	6	7
β-strand	255-259	5	7
β-strand	265-269	5	7
β-strand	278-283	6	8
β-strand	289-294	6	8
β-strand	299-303	5	8
β-strand	309-313	5	8
β-strand	320-325	6	2
β-strand	332-336	5	2
β-strand	341-344	4	2

Chain E: 2 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	12	1	10
β-strand	17-25	9	9
β-strand	35-39	5	11
β-strand	45-51	7	11
β-strand	58-60	3	11
β-strand	68-73	6	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-97	6	11
β-strand	98	1	12
β-strand	110	1	12
β-strand	115-117	3	11
β-strand	119	1	10
β-strand	140-142	3	13
β-strand	146	1	14
β-strand	155-161	7	13
β-strand	166	1	15
β-strand	172	1	15
β-strand	174-179	6	14
β-strand	186-190	5	14
β-strand	194-195	2	14
α-helix	196	1
β-strand	203-207	5	13
β-strand	211-216	6	13
β-strand	226-231	6	14
β-strand	239	1	14
β-strand	243-244	2	14

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-18	10
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain L: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	5-10	6

Chain R: 12 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	60-87	28
α-helix	89-91	3
α-helix	94-122	29
α-helix	131-163	33
α-helix	173-197	25
β-strand	199-200	2	16
β-strand	211-212	2	16
α-helix	221-230	10
α-helix	231-235	5
α-helix	236-254	19
α-helix	267-303	37
α-helix	313-332	20
α-helix	333-337	5
α-helix	340-350	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha-1	A	protein	361	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	371	Homo sapiens	P62873 (AlphaFold model)
SCFV16	E	protein	247	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	70	Homo sapiens	P59768 (AlphaFold model)
Prolactin-releasing peptide PrRP20	L	protein	21	Homo sapiens	P81277 (AlphaFold model)
Prolactin-releasing peptide receptor	R	protein	370	Homo sapiens	P49683

Sequence of entity 1 (A), FASTA

>8ZPS_1 Guanine nucleotide-binding protein G(s) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDVTDIIIKMNLRDCGL
F

Sequence of entity 2 (B), FASTA

>8ZPS_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSG
VSGWRLFKKIS

Sequence of entity 3 (E), FASTA

>8ZPS_3 SCFV16 (chains E)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL

Sequence of entity 4 (G), FASTA

>8ZPS_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFCAIL

Sequence of entity 5 (L), FASTA

>8ZPS_5 Prolactin-releasing peptide PrRP20 (chains L)
TPDINPAWYASRGIRPVGRFX

Sequence of entity 6 (R), FASTA

>8ZPS_6 Prolactin-releasing peptide receptor (chains R)
MASSTTRGPRVSDLFSGLPPAVTTPANQSAEASAGNGSVAGADAPAVTPFQSLQLVHQLK
GLIVLLYSVVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCTACVPLTLAY
AFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIAVDRYVVLVHPLRRRISLRLSAYAV
LAIWALSAVLALPAAVHTYHVELKPHDVRLCEEFWGSQERQRQLYAWGLLLVTYLLPLLV
ILLSYVRVSVKLRNRVVPGCVTQSQADWDRARRRRTFCLLVVIVVVFAVCWLPLHVFNLL
RDLDPHAIDPYAFGLVQLLCHWLAMSSACYNPFIYAWLHDSFREELRKLLVAWPRKIAPH
GQNMTVSVVI

Primary citation

Molecular mechanism of prolactin-releasing peptide recognition and signaling via its G protein-coupled receptor. Li, Y., Yuan, Q., He, X. et al. Cell Discov (2024) 10:91-91. DOI 10.1038/s41421-024-00724-6 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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