8ZST: Lysozyme Crystallized with Bioassembler on Earth

Lysozyme Crystallized with Bioassembler on Earth. Determined by X-ray diffraction at 0.8 Å resolution. Released 28 May 2025.

Method: X-ray diffraction
Resolution: 0.8 Å
Organism: Gallus gallus
Chains: 1
Atoms: 1,585
Mol. weight: 15.75 kDa
Ligands: DO3, GD
Released: 28 May 2025

Explore 8ZST in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8ZST contains 7 α-helices and 9 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	1
α-helix	5-14	10
β-strand	20	1	2
β-strand	23	1	2
α-helix	25-36	12
β-strand	39	1	1
β-strand	43-45	3	3
β-strand	51-53	3	3
β-strand	58-59	2	3
β-strand	65	1	4
β-strand	79	1	4
α-helix	80-84	5
α-helix	89-99	11
α-helix	104-107	4
α-helix	109-114	6
α-helix	121-123	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Lysozyme C	A	protein	129	Gallus gallus	P00698 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8ZST_1 Lysozyme C (chains A)
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINS
RWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDV
QAWIRGCRL

Ligands and cofactors

ID	Name	Formula	Copies
DO3	10-((2R)-2-hydroxypropyl)-1,4,7,10-tetraazacyclododecane 1,4,7-triacetic acid	C17 H32 N4 O7	2
GD	Gadolinium atom	Gd	2

Water and common crystallization additives (NA, CL, NO3) are not listed.

Primary citation

Exploring the potential of a bioassembler for protein crystallization in space. MacCarthy, C., Koudan, E., Shevtsov, M. et al. NPJ Microgravity (2025) 11:25-25. DOI 10.1038/s41526-025-00477-w · PubMed

Other PDB entries of the same protein (UniProt P00698 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2VB1 0.65 Å, HEWL at 0.65 angstrom resolution
9QUM 0.83 Å, Structure of lysozyme by continuous serial electron diffraction (SerialED)
7SKW 0.87 Å, Ab initio structure of triclinic lysozyme from electron-counted MicroED data
7ULY 0.87 Å, MicroED structure of triclinic lysozyme
7P6M 0.89 Å, Hydrogenated refolded hen egg-white lysozyme
9RZL 0.9 Å, Hen egg-white lysozyme (HEWL)
8RLI 0.91 Å, Neutron structure of perdeuterated hen egg-white lysozyme at room temperature
7YRK 0.92 Å, Crystal structure of the hen egg lysozyme-2-oxidobenzylidene-threoninato-copper (II)…
3LZT 0.93 Å, Refinement of triclinic lysozyme at atomic resolution
1IEE 0.94 Å, Structure of tetragonal hen egg white lysozyme at 0.94 a from crystals grown by the…
7OL5 0.94 Å, Crystal structure of Lysozyme in complex with Hepes
4LZT 0.95 Å, Atomic resolution refinement of triclinic hew lysozyme at 295K

Browse structure collections

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