9BHL: Human proton sensing receptor GPR65

Human proton sensing receptor GPR65 in complex with miniGs. Determined by electron microscopy at 2.8 Å resolution. Released 22 Jan 2025.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Homo sapiens, Lama glama
Chains: 4
Atoms: 7,716
Mol. weight: 127.02 kDa
Released: 22 Jan 2025

Explore 9BHL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9BHL contains 29 α-helices and 59 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	10-19	10
α-helix	20-24	5
α-helix	25-39	15
β-strand	41-46	6	1
α-helix	53-63	11
β-strand	207	1	2
β-strand	210-214	5	1
β-strand	217-223	7	1
β-strand	224	1	2
α-helix	234-238	5
β-strand	244-249	6	1
α-helix	253-278	16
β-strand	287-292	6	1
α-helix	295-303	9
α-helix	308-311	4
α-helix	314-317	4
α-helix	332-351	20
β-strand	359-363	5	1
α-helix	371-390	20

Chain B: 1 helix, 34 β-strands

Element	Residues	Length	Sheet
β-strand	49	1	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
α-helix	84-86	3
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	112-116	5	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	175-181	7	6
β-strand	187	1	7
β-strand	191-192	2	8
β-strand	198-202	5	8
β-strand	203	1	7
β-strand	208-212	5	8
β-strand	218-222	5	8
β-strand	232-234	3	9
β-strand	240-243	4	9
β-strand	249-252	4	10
β-strand	253-254	2	9
β-strand	262-265	4	10
β-strand	273-278	6	11
β-strand	285-289	5	11
β-strand	294	1	11
β-strand	297	1	12
β-strand	304	1	12
β-strand	315-317	3	13
β-strand	320	1	3
β-strand	327-328	2	3
β-strand	330-331	2	13
β-strand	338-339	2	3

Chain N: 2 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	14
β-strand	10-12	3	15
β-strand	13	1	16
β-strand	16	1	16
β-strand	19-25	7	14
β-strand	34-39	6	17
β-strand	45-51	7	17
α-helix	53-55	3
β-strand	58-59	2	17
β-strand	68	1	18
β-strand	71-73	3	14
β-strand	78-82	5	14
β-strand	83	1	18
α-helix	88-90	3
β-strand	93-98	6	17
β-strand	118	1	17
β-strand	124-126	3	15

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	7-44	38
α-helix	49-74	26
α-helix	84-112	29
α-helix	113-117	5
α-helix	128-146	19
α-helix	148-151	4
β-strand	155-160	6	19
β-strand	165-170	6	19
α-helix	176-177	2
α-helix	178-191	14
α-helix	193-212	20
α-helix	218-255	38
α-helix	262-268	7
α-helix	269-279	11
α-helix	281-287	7
α-helix	288-292	5
α-helix	297-304	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	261	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	370	Homo sapiens	P62873 (AlphaFold model)
Nanobody 35	N	protein	142	Lama glama
Psychosine receptor	R	protein	348	Homo sapiens	Q8IYL9 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9BHL_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
GGSLEVLFQGPSGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSG
KSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTA
IIFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFP
EFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENAR
RIFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>9BHL_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPEDQVDPRLIDGKGSSQSELDQLRQEAEQLKNQIRDARKACADATL
SQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKV
HAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCR
FLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKL
WDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNII
CGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATG
SWDSFLKIWN

Sequence of entity 3 (N), FASTA

>9BHL_3 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSGSEDQVDPRLIDGK

Sequence of entity 4 (R), FASTA

>9BHL_4 Psychosine receptor (chains R)
DYKDDDDASIDMNSTCIEEQHDLDHYLFPIVYIFVIIVSIPANIGSLCVSFLQAKKESEL
GIYLFSLSLSDLLYALTLPLWIDYTWNKDNWTFSPALCKGSAFLMYMNFYSSTAFLTCIA
VDRYLAVVYPLKFFFLRTRRFALMVSLSIWILETIFNAVMLWEDETVVEYCDAEKSNFTL
CYDKYPLEKWQINLNLFRTCTGYAIPLVTILICNRKVYQAVRHNKATENKEKKRIIKLLV
SITVTFVLCFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTSLNCVADPIL
YCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLE

Primary citation

Molecular basis of proton sensing by G protein-coupled receptors. Howard, M.K., Hoppe, N., Huang, X.P. et al. Cell (2025) 188:671. DOI 10.1016/j.cell.2024.11.036 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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