9BLR: Human SCNN1D-SCNN1B-SCNN1G ENaC trimer

Human SCNN1D-SCNN1B-SCNN1G ENaC trimer. Determined by electron microscopy at 3.38 Å resolution. Released 27 Nov 2024.

Method
Electron microscopy
Resolution
3.38 Å
Organism
Homo sapiens
Chains
3
Atoms
9,426
Mol. weight
220.68 kDa
Ligands
NAG
Released
27 Nov 2024

Explore 9BLR in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9BLR contains 48 α-helices and 70 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 22 β-strands

ElementResiduesLengthSheet
β-strand130-13451
α-helix142-1443
α-helix145-16218
β-strand193-19532
β-strand205-21172
β-strand214-22292
α-helix225-24117
β-strand260-26563
β-strand268-27033
α-helix272-2743
β-strand276-28051
β-strand284-28851
α-helix294-2952
α-helix300-3023
β-strand304-30963
β-strand32314
β-strand325-33061
β-strand343-34531
β-strand349-361133
β-strand37115
β-strand37216
β-strand37516
α-helix389-40517
β-strand40817
β-strand42017
α-helix429-44113
α-helix447-4504
α-helix452-4532
β-strand45415
β-strand456-468133
α-helix475-48511
β-strand498-50583
β-strand512-51323
Chain B: 21 helices, 25 β-strands
ElementResiduesLengthSheet
β-strand79-8684
β-strand90-9128
α-helix92-932
β-strand94-9969
α-helix105-1084
α-helix113-12715
α-helix129-1313
α-helix142-1454
β-strand150-154510
β-strand162-165410
α-helix182-1843
β-strand189-197910
β-strand202-208710
α-helix211-22717
α-helix232-2354
α-helix242-2454
β-strand246-25164
β-strand254-25634
α-helix258-2603
β-strand262-26659
β-strand270-27569
α-helix282-2843
β-strand285-28628
α-helix291-2933
β-strand295-30064
α-helix303-3053
β-strand314111
β-strand316-32169
α-helix329-3324
β-strand334-33639
β-strand340-352134
β-strand362112
α-helix373-3775
α-helix379-3813
α-helix383-39917
β-strand402113
β-strand414113
α-helix423-43210
α-helix434-4418
α-helix444-4463
β-strand447112
β-strand449-45024
β-strand45314
β-strand456-46164
α-helix468-47710
β-strand492-511204
Chain C: 16 helices, 23 β-strands
ElementResiduesLengthSheet
β-strand82-89811
β-strand92-93214
β-strand96-101615
α-helix107-12822
α-helix153-1575
β-strand160-162316
β-strand202-206516
β-strand215-219516
α-helix222-23817
α-helix243-2486
α-helix253-2564
β-strand257-262611
β-strand265-267311
α-helix269-2713
β-strand272-277615
β-strand281-286615
α-helix293-2953
β-strand296-297214
α-helix302-3043
β-strand306-311611
α-helix314-3163
β-strand32513
β-strand327-332615
α-helix340-3434
β-strand345-347315
β-strand351-3631311
β-strand373117
α-helix391-40515
β-strand410118
β-strand422118
α-helix431-44212
α-helix449-4524
β-strand456117
β-strand458-462511
β-strand465-470611
α-helix477-48711
α-helix495-4973
α-helix498-5003
β-strand501-5191911

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Isoform 1 of Amiloride-sensitive sodium channel subunit deltaAprotein638Homo sapiensP51172 (AlphaFold model)
Amiloride-sensitive sodium channel subunit betaBprotein640Homo sapiensP51168 (AlphaFold model)
Amiloride-sensitive sodium channel subunit gammaCprotein649Homo sapiensP51170 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>9BLR_1 Isoform 1 of Amiloride-sensitive sodium channel subunit delta (chains A)
MAEHRSMDGRMEAATRGGSHLQAAAQTPPRPGPPSAPPPPPKEGHQEGLVELPASFRELL
TFFATNATIHGAIRLVCSRGNRLKTTSWGLLSLGALVALCWQLGLLFERHWHRPVLMAVS
VHSERKLLPLVTLCDGNPRRPSPVLRHLELLDEFARENIDSLYNVNLSKGRAALSATVPR
HEPPFHLDREIRLQRLSHSGSRVRVGFRLCNSTGGDCFYRGYTSGVAAVQDWYHFHYVDI
LALLPAAWEDSHGSQDGHFVLSCSYDGLDCQARQFRTFHHPTYGSCYTVDGVWTAQRPGI
THGVGLVLRVEQQPHLPLLSTLAGIRVMVHGRNHTPFLGHHSFSVRPGTEATISIREDEV
HRLGSPYGHCTAGGEGVEVELLHNTSYTRQACLVSCFQQLMVETCSCGYYLHPLPAGAEY
CSSARHPAWGHCFYRLYQDLETHRLPCTSRCPRPCRESAFKLSTGTSRWPSAKSAGWTLA
TLGEQGLPHQSHRQRSSLAKINIVYQELNYRSVEEAPVYSVPQLLSAMGSLCSLWFGASV
LSLLELLELLLDASALTLVLGGRRLRRAWFSWPRASPASGASSIKPEASQMPPPAGGTSD
DPEPSGPHLPRVMLPGVLAGVSAEESWAGPQPLETLDT
Sequence of entity 2 (B), FASTA
>9BLR_2 Amiloride-sensitive sodium channel subunit beta (chains B)
MHVKKYLLKGLHRLQKGPGYTYKELLVWYADNTNTHGPKRIICEGPKKKAMWFLLTLLFA
ALVCWQWGIFIRTYLSWEVSVSLSVGFKTMDFPAVTICNASPFKYSKIKHLLKDLDELME
AVLERILAPELSHANATRNLNFSIWNHTPLVLIDERNPHHPMVLDLFGDNHNGLTSSSAS
EKICNAHGCKMAMRLCSLNRTQCTFRNFTSATQALTEWYILQATNIFAQVPQQELVEMSY
PGEQMILACLFGAEPCNYRNFTSIFYPHYGNCYIFNWGMTEKALPSANPGTEFGLKLILD
IGQEDYVPFLASTAGVRLMLHEQRSYPFIRDEGIYAMSGTETSIGVLVDKLQRMGEPYSP
CTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRGEKYCNNRDF
PDWAHCYSDLQMSVAQRETCIGMCKESCNDTQYKMTISMADWPSEASEDWIFHVLSQERD
QSTNITLSRKGIVKLNIYFQEFNYRTIEESAANNIVWLLSNLGGQFGFWMGGSVLCLIEF
GEIIIDFVWITIIKLVALAKSLRQRRAQASYAGPPPTVAELVEAHTNFGFQPDTAPRSPN
TGPYPSEQALPIPGTPPPNYDSLRLQPLDVIESDSEGDAI
Sequence of entity 3 (C), FASTA
>9BLR_3 Amiloride-sensitive sodium channel subunit gamma (chains C)
MAPGEKIKAKIKKNLPVTGPQAPTIKELMRWYALNTNTHGARRIVVSRGRLRRLLWIGFT
LTAVALILWQCALLVFSFYTVSVSIKVHFRKLDFPAVTICNINPYKYSTVRHLLADLEQE
TREALKSLYGFPESRKRAEAESWNSVSEGKQPRFSHRIPLLIFDQDEKGKARDFFTGRKR
KVGGSIIHKASNVMHIESKQVVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQ
VPLEKKINMSYSAEELLVTCFFDGVSCDARNFTLFHHPMHGNCYTFNNRENETILSTSMG
GSEYGLQVILYINEEEYNPFLVSSTGAKVIIHRQDEYPFVEDVGTEIETAMVTSIGMHLT
ESFKLSEPYSQCTEDGSDVPIRNIYNAAYSLQICLHSCFQTKMVEKCGCAQYSQPLPPAA
NYCNYQQHPNWMYCYYQLHRAFVQEELGCQSVCKEACSFKEWTLTTSLAQWPSVVSEKWL
LPVLTWDQGRQVNKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMS
CSVVCVIEIIEVFFIDFFSIIARRQWQKAKEWWAWKQAPPCPEAPRSPQGQDNPALDIDD
DLPTFNSALHLPPALGTQVPGTPPPKYNTLRLERAFSNQLTDTQMLDEL

Ligands and cofactors

IDNameFormulaCopies
NAG2-acetamido-2-deoxy-beta-D-glucopyranoseC8 H15 N O66

Primary citation

Structural insights into subunit-dependent functional regulation in epithelial sodium channels. Houser, A., Baconguis, I. Structure (2025) 33:349-362.e4. DOI 10.1016/j.str.2024.11.013 · PubMed

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