9FQZ: HCT15 polysomes

cryo-EM structure of HCT15 polysomes bound to EEF2, EBP1, and SERBP1. Determined by electron microscopy at 2.85 Å resolution. Released 14 Jan 2026.

Method: Electron microscopy
Resolution: 2.85 Å
Organism: Homo sapiens
Chains: 84
Atoms: 216,008
Mol. weight: 4049.2 kDa
Ligands: GDP, MG, ZN, PUT
Released: 14 Jan 2026

Explore 9FQZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9FQZ contains 611 α-helices and 600 β-strands across 79 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain CA: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	289-298	10

Chain CB: 44 helices, 40 β-strands

Element	Residues	Length	Sheet
α-helix	6-14	9
α-helix	16-18	3
β-strand	19-26	8	1
α-helix	32-42	11
β-strand	75-80	6	1
α-helix	83-86	4
β-strand	97-104	8	1
α-helix	112-121	10
β-strand	124-130	7	1
α-helix	137-148	12
β-strand	152-158	7	1
α-helix	160-165	6
α-helix	171-191	21
α-helix	207-209	3
β-strand	212-216	5	1
β-strand	221-224	4	1
α-helix	225-235	11
α-helix	248-259	12
β-strand	265-267	3	2
β-strand	272-274	3	2
β-strand	278	1	3
β-strand	284	1	3
β-strand	287	1	2
α-helix	288-289	2
α-helix	290-294	5
α-helix	295-306	12
α-helix	309-319	11
α-helix	333-343	11
β-strand	345	1	1
α-helix	346-357	12
α-helix	359-360	2
α-helix	361-372	12
β-strand	373	1	4
α-helix	380-387	8
β-strand	395-403	9	5
α-helix	404	1
β-strand	410-418	9	5
β-strand	420-422	3	6
β-strand	426-430	5	5
β-strand	442-446	5	5
β-strand	449-454	6	5
β-strand	457-460	4	5
β-strand	463-465	3	6
β-strand	469-473	5	5
β-strand	483-486	4	5
α-helix	491-493	3
β-strand	494	1	4
α-helix	495-497	3
β-strand	505-511	7	7
α-helix	514-516	3
α-helix	517-530	14
β-strand	535-538	4	7
β-strand	544-548	5	7
α-helix	551-560	10
α-helix	561-565	5
β-strand	570-573	4	7
α-helix	574-576	3
β-strand	580-583	4	8
β-strand	591-594	4	9
β-strand	601-608	8	9
α-helix	609-610	2
α-helix	613-619	7
α-helix	628-639	12
α-helix	643-646	4
β-strand	649-652	4	9
α-helix	654-656	3
β-strand	660-664	5	9
α-helix	672-686	15
α-helix	695-696	2
β-strand	697	1	8
β-strand	700-708	9	9
α-helix	713-715	3
α-helix	718-734	17
β-strand	738-752	15	8
α-helix	757-765	9
β-strand	769-775	7	8
β-strand	781-789	9	8
α-helix	796-804	9
β-strand	809-820	12	8
α-helix	830-842	13
α-helix	844-845	2
α-helix	848-850	3
α-helix	851-853	3
α-helix	855-857	3

Chain CD: 4 helices, 3 β-strands

Element	Residues	Length	Sheet
β-strand	185	1	32
β-strand	191	1	32
α-helix	210-212	3
α-helix	222-225	4
β-strand	282	1	33
α-helix	284-293	10
α-helix	302-304	3

Chain La: 9 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	3-5	3
α-helix	7-10	4
α-helix	42-48	7
α-helix	65-68	4
β-strand	72-73	2	37
α-helix	75-77	3
α-helix	79-81	3
α-helix	84-92	9
β-strand	98	1	121
β-strand	100-102	3	38
α-helix	103-106	4
β-strand	110-111	2	37
β-strand	123-126	4	38
β-strand	128-129	2	37
α-helix	131-140	10
β-strand	143-146	4	38

Chain LA: 12 helices, 18 β-strands

Element	Residues	Length	Sheet
α-helix	4-5	2
α-helix	9-12	4
α-helix	29-32	4
α-helix	34-38	5
β-strand	40-49	10	42
α-helix	50-51	2
β-strand	58-64	7	42
β-strand	71-77	7	42
α-helix	78	1
β-strand	79	1	43
β-strand	83	1	44
β-strand	87-91	5	42
β-strand	101-103	3	42
α-helix	104-106	3
β-strand	112-113	2	45
β-strand	116	1	46
β-strand	126	1	46
β-strand	134-140	7	45
β-strand	145-149	5	45
β-strand	155-159	5	45
β-strand	163-165	3	42
β-strand	166	1	45
α-helix	168	1
β-strand	169	1	43
α-helix	174-176	3
α-helix	182-190	9
α-helix	201-203	3
β-strand	224-225	2	47
α-helix	229-230	2
β-strand	237-238	2	47

Chain Lb: 7 helices, 0 β-strands

Element	Residues	Length
α-helix	12-18	7
α-helix	22-24	3
α-helix	37-48	12
α-helix	49-51	3
α-helix	54-71	18
α-helix	92-100	9
α-helix	106-117	12

Chain LB: 18 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	9-10	2
α-helix	14-16	3
α-helix	33-35	3
α-helix	40-41	2
β-strand	42-43	2	55
β-strand	45-60	16	56
β-strand	70-80	11	56
α-helix	82-83	2
β-strand	84-95	12	55
β-strand	98-106	9	55
α-helix	112-115	4
α-helix	116-118	3
α-helix	133-137	5
α-helix	140-156	17
β-strand	159-165	7	55
α-helix	168-170	3
α-helix	177-179	3
β-strand	180-186	7	55
α-helix	190-200	11
β-strand	204-205	2	55
α-helix	207-209	3
β-strand	216-222	7	56
α-helix	223-224	2
β-strand	225-229	5	57
α-helix	231-234	4
α-helix	237-241	5
β-strand	270-273	4	57
β-strand	276-288	13	56
β-strand	292-293	2	58
β-strand	298-299	2	58
β-strand	320	1	59
β-strand	324	1	59
β-strand	330-334	5	56
β-strand	344-349	6	56
β-strand	365-368	4	56
α-helix	382-389	8
α-helix	393-399	7

Chain Lc: 4 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	14-24	11
β-strand	26-29	4	60
α-helix	31-39	9
β-strand	45-48	4	60
α-helix	54-67	14
β-strand	70-73	4	60
α-helix	78-84	7
β-strand	93-98	6	60

71 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Elongation factor 2	CB	protein	858	Homo sapiens	P13639 (AlphaFold model)
tRNA	CC	RNA	85	Homo sapiens
Isoform 2 of SERPINE1 mRNA-binding protein 1	CD	protein	402	Homo sapiens	Q8NC51 (AlphaFold model)
5.8S rRNA	L8	RNA	156	Homo sapiens
60S ribosomal protein L8	LA	protein	257	Homo sapiens	P62917 (AlphaFold model)
60S ribosomal protein L3	LB	protein	403	Homo sapiens	P39023 (AlphaFold model)
60S ribosomal protein L4	LC	protein	427	Homo sapiens	P36578
60S ribosomal protein L5	LD	protein	297	Homo sapiens	P46777
60S ribosomal protein L6	LE	protein	288	Homo sapiens	Q02878
Large ribosomal subunit protein uL30	LF	protein	248	Homo sapiens	P18124
60S ribosomal protein L7a	LG	protein	266	Homo sapiens	P62424
60S ribosomal protein L9	LH	protein	192	Homo sapiens	P32969

72 more molecules are not listed.

Sequence of entity 1 (CB), FASTA

>9FQZ_1 Elongation factor 2 (chains CB)
MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTR
KDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALR
VTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQR
IVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKG
EGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKV
FDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPS
PVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGL
VSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLV
KTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEE
SGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHN
RLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPN
ILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQ
IIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTP
MFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRK
RKGLKEGIPALDNFLDKL

Sequence of entity 2 (CC), FASTA

>9FQZ_2 tRNA (chains CC)
GUAGUCGUGGCCGAGUGGUUAAGGCGAUGGACUUGAAAUCCAUUGGGGUCUCCCCGCGCA
GGUUCGAAUCCUGCCGACUACGCCA

Sequence of entity 3 (CD), FASTA

>9FQZ_3 Isoform 2 of SERPINE1 mRNA-binding protein 1 (chains CD)
MPGHLQEGFGCVVTNRFDQLFDDESDPFEVLKAAENKKKEAGGGGVGGPGAKSAAQAAAQ
TNSNAAGKQLRKESQKDRKNPLPPSVGVVDKKEETQPPVALKKEGIRRVGRRPDQQLQGE
GKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMG
RGDGFDSRGKREFDRHSGSDRSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNY
SDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEF
NIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPG
RGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA

Sequence of entity 4 (L8), FASTA

>9FQZ_4 5.8S rRNA (chains L8)
CGACUCUUAGCGGUGGAUCACUCGGCUCGUGCGUCGAUGAAGAACGCAGCUAGCUGCGAG
AAUUAAUGUGAAUUGCAGGACACAUUGAUCAUCGACACUUCGAACGCACUUGCGGCCCCG
GGUUCCUCCCGGGGCUACGCCUGUCUGAGCGUCGCU

Sequence of entity 5 (LA), FASTA

>9FQZ_5 60S ribosomal protein L8 (chains LA)
MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAK
VVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKP
GDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPIL
KAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAA
RRTGRLRGTKTVQEKEN

Sequence of entity 6 (LB), FASTA

>9FQZ_6 60S ribosomal protein L3 (chains LB)
MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREV
DRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYK
NWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHL
MEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKL
PRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIK
NNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRAL
EKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA

Sequence of entity 7 (LC), FASTA

>9FQZ_7 60S ribosomal protein L4 (chains LC)
MACARPLISVYSEKGESSGKNVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGH
QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQK
RYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVLLLKKLKAWNDI
KKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKL
APGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMINTDLSRILKSPEIQR
ALRAPRKKIHRRVLKKNPLKNLRIMLKLNPYAKTMRRNTILRQARNHKLRVDKAAAAAAA
LQAKSDEKAAVAGKKPVVGKKGKKAAVGVKKQKKPLVGKKAAATKKPAPEKKPAEKKPTT
EEKKPAA

Sequence of entity 8 (LD), FASTA

>9FQZ_8 60S ribosomal protein L5 (chains LD)
MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDI
ICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYE
GQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRF
PGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMY
KKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES

Sequence of entity 9 (LE), FASTA

>9FQZ_9 60S ribosomal protein L6 (chains LE)
MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRS
AMYSRKAMYKRKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTED
VPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLV
LNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKY
EITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF

Sequence of entity 10 (LF), FASTA

>9FQZ_10 Large ribosomal subunit protein uL30 (chains LF)
MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKE
YRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNG
TFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLG
KYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQI
NRLIRRMN

Sequence of entity 11 (LG), FASTA

>9FQZ_11 60S ribosomal protein L7a (chains LG)
MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRY
IRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEK
KAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVP
YCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGN
VLGPKSVARIAKLEKAKAKELATKLG

Sequence of entity 12 (LH), FASTA

>9FQZ_12 60S ribosomal protein L9 (chains LH)
MKTILSNQTVDIPENVDITLKGRTVIVKGPRGTLRRDFNHINVELSLLGKKKKRLRVDKW
WGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYAHFPINVVIQENGSLVEIRNFLGE
KYIRRVRMRPGVACSVSQAQKDELILEGNDIELVSNSAALIQQATTVKNKDIRKFLDGIY
VSEKGTVQQADE

Ligands and cofactors

ID	Name	Formula	Copies
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1
MG	Magnesium ion	Mg	271
ZN	Zinc ion	Zn	8
PUT	1,4-diaminobutane	C4 H12 N2	7
HYG	Hygromycin B	C20 H37 N3 O13	1
SPD	Spermidine	C7 H19 N3	8
3HE	4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2…	C15 H23 N O4	1
ANM	Anisomycin	C14 H19 N O4	1

Water and common crystallization additives (K, NA) are not listed.

Primary citation

CRYO-EM STRUCTURE OF HCT15 POLYSOMES BOUND TO EEF2, EBP1, AND SERBP1. Rajan, K.S., Yonath, A. To be published.

Other PDB entries of the same protein (UniProt P13639 (AlphaFold model), which also has an AlphaFold model), best resolution first:

29IZ 2.08 Å, Dormant 80S:eEF2:SERBP1:tRNA complex from human cells treated with toxin NT-2
11IQ 2.4 Å, Rabbit 80S with eEF2,eIF5A and SERBP1
8XSX 2.4 Å, Cryo-EM structure of the human 80S ribosome with Tigecycline, E-tRNA, SERBP1 and eEF2
9P7D 2.57 Å, In situ human Hibernating class4 80S ribosome
9P7E 2.59 Å, In situ human Hibernating class5 80S ribosome
9P73 2.66 Å, In situ human eEF2-A/P-P/E state 80S ribosome
9T6D 2.67 Å, NAA40-NAC bound human 80S (combined translation states)
9P9I 2.77 Å, In situ human unrotated hibernating without CCDC124 state 80S ribosome
9M0P 2.78 Å, Cryo-EM structure of human 80S ribosome in complex with montanine
9P7C 2.78 Å, In situ human Hibernating class3 80S ribosome
9P7A 2.81 Å, In situ human hibernating class2 80S ribosome
9B0P 2.82 Å, In situ human Hibernating class1 80S ribosome

Browse structure collections

About this viewer

MolViewer shows 9FQZ directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.