9K26: Prolactin-releasing peptide receptor
PrRP31 bound prolactin-releasing peptide receptor coupled with Gi protein complex. Determined by electron microscopy at 3.0 Å resolution. Released 29 Jan 2025.
- Method
- Electron microscopy
- Resolution
- 3.0 Å
- Organism
- Homo sapiens
- Chains
- 6
- Atoms
- 8,967
- Mol. weight
- 159.84 kDa
- Released
- 29 Jan 2025
Explore 9K26 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9K26 contains 36 α-helices and 67 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 13 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 55-58 | 4 | |
| α-helix | 60-87 | 28 | |
| α-helix | 94-120 | 27 | |
| β-strand | 126 | 1 | 1 |
| α-helix | 131-164 | 34 | |
| α-helix | 169-172 | 4 | |
| α-helix | 173-195 | 23 | |
| β-strand | 201-203 | 3 | 1 |
| β-strand | 208-210 | 3 | 1 |
| α-helix | 219-233 | 15 | |
| α-helix | 235-253 | 19 | |
| α-helix | 266-301 | 36 | |
| α-helix | 305-307 | 3 | |
| α-helix | 313-332 | 20 | |
| α-helix | 333-337 | 5 | |
| α-helix | 340-350 | 11 | |
Chain B: 5 helices, 31 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-25 | 21 | |
| α-helix | 30-33 | 4 | |
| α-helix | 38-39 | 2 | |
| β-strand | 51 | 1 | 3 |
| β-strand | 58-63 | 6 | 4 |
| β-strand | 69-74 | 6 | 4 |
| β-strand | 78-83 | 6 | 4 |
| β-strand | 89-94 | 6 | 4 |
| β-strand | 100-105 | 6 | 5 |
| β-strand | 111-116 | 6 | 5 |
| β-strand | 121-125 | 5 | 5 |
| β-strand | 134-139 | 6 | 5 |
| α-helix | 145 | 1 | |
| β-strand | 146-153 | 8 | 6 |
| β-strand | 156-161 | 6 | 6 |
| β-strand | 165-168 | 4 | 6 |
| β-strand | 178-181 | 4 | 6 |
| β-strand | 187-192 | 6 | 7 |
| β-strand | 199-203 | 5 | 7 |
| β-strand | 207-210 | 4 | 7 |
| β-strand | 220-223 | 4 | 7 |
| β-strand | 229-234 | 6 | 8 |
| β-strand | 240-245 | 6 | 8 |
| β-strand | 250-254 | 5 | 8 |
| β-strand | 260-264 | 5 | 8 |
| α-helix | 272 | 1 | |
| β-strand | 273-277 | 5 | 9 |
| β-strand | 285-289 | 5 | 9 |
| β-strand | 294-298 | 5 | 9 |
| β-strand | 303-308 | 6 | 9 |
| β-strand | 315 | 1 | 10 |
| β-strand | 319-320 | 2 | 11 |
| β-strand | 327-328 | 2 | 11 |
| β-strand | 331 | 1 | 10 |
| β-strand | 336 | 1 | 3 |
| β-strand | 339 | 1 | 11 |
Chain C: 9 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-31 | 25 | |
| β-strand | 34-38 | 5 | 2 |
| β-strand | 185-190 | 6 | 2 |
| β-strand | 195-200 | 6 | 2 |
| α-helix | 208-210 | 3 | |
| α-helix | 212-215 | 4 | |
| β-strand | 220-226 | 7 | 2 |
| α-helix | 228-231 | 4 | |
| α-helix | 242-254 | 13 | |
| β-strand | 263-269 | 7 | 2 |
| α-helix | 271-278 | 8 | |
| α-helix | 283-285 | 3 | |
| α-helix | 296-309 | 14 | |
| β-strand | 319-320 | 2 | 2 |
| α-helix | 330-350 | 21 | |
Chain F: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 18-22 | 5 | |
Chain G: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-23 | 15 | |
| α-helix | 30-43 | 14 | |
| α-helix | 53-55 | 3 | |
Chain S: 5 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 6-7 | 2 | 12 |
| β-strand | 10 | 1 | 13 |
| β-strand | 17-23 | 7 | 12 |
| α-helix | 29-31 | 3 | |
| β-strand | 33-35 | 3 | 14 |
| β-strand | 38-39 | 2 | 13 |
| β-strand | 45-46 | 2 | 13 |
| β-strand | 49 | 1 | 15 |
| β-strand | 50-51 | 2 | 14 |
| β-strand | 60 | 1 | 15 |
| β-strand | 68-72 | 5 | 12 |
| α-helix | 74-76 | 3 | |
| β-strand | 78-84 | 7 | 12 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-94 | 3 | 13 |
| β-strand | 98-99 | 2 | 14 |
| β-strand | 110 | 1 | 14 |
| β-strand | 115-117 | 3 | 13 |
| β-strand | 141 | 1 | 16 |
| β-strand | 146-148 | 3 | 17 |
| β-strand | 157-159 | 3 | 18 |
| β-strand | 160 | 1 | 16 |
| β-strand | 174-179 | 6 | 17 |
| β-strand | 187-190 | 4 | 17 |
| β-strand | 194-195 | 2 | 17 |
| α-helix | 196 | 1 | |
| β-strand | 205-207 | 3 | 18 |
| β-strand | 212-214 | 3 | 18 |
| β-strand | 225-231 | 7 | 17 |
| α-helix | 237 | 1 | |
| β-strand | 239 | 1 | 17 |
| β-strand | 243-246 | 4 | 17 |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Prolactin-releasing peptide receptor | A | protein | 370 | Homo sapiens | P49683 (AlphaFold model) |
| Prolactin-releasing peptide PrRP31 | F | protein | 32 | Homo sapiens | P81277 (AlphaFold model) |
| Guanine nucleotide-binding protein G(i) subunit alpha-1 | C | protein | 351 | Homo sapiens | P63096 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 357 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 |
| scfv16 | S | protein | 261 | Homo sapiens | |
Sequence of entity 1 (A), FASTA
>9K26_1 Prolactin-releasing peptide receptor (chains A)
MASSTTRGPRVSDLFSGLPPAVTTPANQSAEASAGNGSVAGADAPAVTPFQSLQLVHQLK
GLIVLLYSVVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCTACVPLTLAY
AFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIAVDRYVVLVHPLRRRISLRLSAYAV
LAIWALSAVLALPAAVHTYHVELKPHDVRLCEEFWGSQERQRQLYAWGLLLVTYLLPLLV
ILLSYVRVSVKLRNRVVPGCVTQSQADWDRARRRRTFCLLVVIVVVFAVCWLPLHVFNLL
RDLDPHAIDPYAFGLVQLLCHWLAMSSACYNPFIYAWLHDSFREELRKLLVAWPRKIAPH
GQNMTVSVVI
Sequence of entity 2 (F), FASTA
>9K26_2 Prolactin-releasing peptide PrRP31 (chains F)
SRTHRHSMEIRTPDINPAWYASRGIRPVGRFX
Sequence of entity 3 (C), FASTA
>9K26_3 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains C)
TLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSE
EECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAEL
AGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTG
IVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRM
HESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYI
QCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF
Sequence of entity 4 (B), FASTA
>9K26_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRI
QMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTC
AYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFT
GHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGR
LLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 5 (G), FASTA
>9K26_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 6 (S), FASTA
>9K26_6 scfv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQGPAAA
Primary citation
a peptide receptor complex structure. Wu, Z., Du, Y., Jun, X. To be published.
Other PDB entries of the same protein (UniProt P49683 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 9V0X 2.66 Å, Cryo-EM structure of Gq-coupled PrRPR in complex with GUB08248
- 9K27 2.68 Å, PrRP31 bound prolactin-releasing peptide receptor coupled with Gq protein complex
- 8ZPT 2.96 Å, Cryo-EM structure of prolactin-releasing peptide recognition with Gq
- 8ZPS 2.97 Å, Cryo-EM structure of prolactin-releasing peptide recognition with Gi
Browse structure collections
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