9LLE: D1R-Gs

Cryo-EM structure of D1R-Gs in complex with de novo designed GEM targeting TM1/2/4. Determined by electron microscopy at 2.9 Å resolution. Released 4 Mar 2026.

Method: Electron microscopy
Resolution: 2.9 Å
Organisms: Homo sapiens, synthetic construct, Lama glama
Chains: 6
Atoms: 8,527
Mol. weight: 200.24 kDa
Ligands: LDP
Released: 4 Mar 2026

Explore 9LLE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9LLE contains 38 α-helices and 49 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
α-helix	30-36	7
β-strand	49-51	3	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	89-94	6	2
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	120-125	6	3
β-strand	134-140	7	3
β-strand	146-151	6	4
β-strand	156-161	6	4
β-strand	166-170	5	4
β-strand	175-180	6	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	208-212	5	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	249-254	6	6
β-strand	259-264	6	6
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	304-308	5	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain C: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	6-23	18
α-helix	30-44	15
α-helix	45-47	3
α-helix	53-55	3
α-helix	56-58	3

Chain D: 10 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	12-38	27
β-strand	40-47	8	12
β-strand	209-214	6	12
β-strand	217-223	7	12
α-helix	234-238	5
β-strand	243-249	7	12
α-helix	265-277	13
α-helix	280-282	3
β-strand	286-292	7	12
α-helix	294-303	10
α-helix	308-310	3
α-helix	313-317	5
α-helix	326-327	2
α-helix	332-351	20
β-strand	359	1	12
β-strand	362-363	2	12
α-helix	372-390	19

Chain N: 2 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	3-8	6	8
β-strand	10-12	3	9
β-strand	18-25	8	8
α-helix	29-31	3
β-strand	34	1	10
β-strand	36-39	4	9
β-strand	45-50	6	9
β-strand	59-60	2	9
β-strand	68-73	6	8
β-strand	78-83	6	8
α-helix	88-90	3
β-strand	92-95	4	9
β-strand	97	1	11
β-strand	98	1	10
β-strand	118	1	11
β-strand	122-126	5	9

Chain O: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	2-30	29
α-helix	34-68	35

Chain R: 17 helices, 0 β-strands

Element	Residues	Length
α-helix	21-50	30
α-helix	52-55	4
α-helix	59-72	14
α-helix	73-77	5
α-helix	78-87	10
α-helix	93-95	3
α-helix	96-126	31
α-helix	128-134	7
α-helix	137-156	20
α-helix	192-199	8
α-helix	200-204	5
α-helix	205-233	29
α-helix	266-294	29
α-helix	295-297	3
α-helix	310-321	12
α-helix	323-331	9
α-helix	335-345	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
D(1A) dopamine receptor,Expression tag	R	protein	619	Homo sapiens, synthetic construct	P21728 (AlphaFold model)
De novo designed GPCR exoframe modulator targeting TM1/2/4	O	protein	203	synthetic construct
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	384	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanoboy 35	N	protein	138	Lama glama
Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	D	protein	380	Homo sapiens	P63092 (AlphaFold model)

Sequence of entity 1 (R), FASTA

>9LLE_1 D(1A) dopamine receptor,Expression tag (chains R)
RTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNF
FVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDR
YWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAE
TIDNCDSSLSRTYAISSSVISFYIPVAIMIVTYTRIYRIAQKQIRRIAALERAAVHAKNC
QTTTGNGKPVECSQPESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCILPFCGSG
ETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNAIETV
SINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPALS
VILDYDTDVSLEKIQPITQNGQHPTGSGGSGGGGSGGSGGSVFTLEDFVGDWEQTAAYNL
DQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFK
VVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKII
DERLITPDGSMLFRVTINS

Sequence of entity 2 (O), FASTA

>9LLE_2 De novo designed GPCR exoframe modulator targeting TM1/2/4 (chains O)
DYKDDDDKEFADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLED
KSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLLEVL
FQGPMLLIIGTIITLVSSIIFLISFFRFMRKWIRGLTRRDVRRFLVVLLVFFLLFLISLL
LYVLFLVLYFLSLGKIDPKTGSA

Sequence of entity 3 (B), FASTA

>9LLE_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGS
SGGGGSGGGGSSGVSGWRLFKKIS

Sequence of entity 4 (C), FASTA

>9LLE_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (N), FASTA

>9LLE_5 Nanoboy 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 6 (D), FASTA

>9LLE_6 Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains D)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSV
MNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSD
QDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVASSSYN
MVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEKVLAGKSKIEDYFPE
FARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRR
VFNDCRDIIQRMHLRQYELL

Ligands and cofactors

ID	Name	Formula	Copies
LDP	L-dopamine	C8 H11 N O2	1

Primary citation

GPCR exoframe modulator. Cheng, S., Guo, J., Zhou, Y. et al. To be published.

Other PDB entries of the same protein (UniProt P21728 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
9WG0 2.59 Å, D1R-Gs in complexed with SKF81297/LY3154207/BMSA1/UNC10062
9LLJ 2.61 Å, Cryo-EM structure of D1R-Gs in complex with de novo designed GEM targetingTM1/2/4 and…
9LLF 2.64 Å, Cryo-EM structure of D1R-Gs in complex with de novo designed GEM targeting TM3/4/5
9WEU 2.65 Å, D1R-Gs in complexed with SKF81297/LY3154207/BMSA1
9I54 2.72 Å, Dopamine 1 receptor:GaS complex bound to 24
9LLH 2.73 Å, Cryo-EM structure of D1R-Gs in complex with de novo designed agonist-positive allosteric…
9LLG 2.77 Å, Cryo-EM structure of D1R in complex with de novo designed negative allosteric GEM…
9I52 2.8 Å, Dopamine 1 receptor:GaS complex bound to 19B
7JVP 2.9 Å, Cryo-EM structure of SKF-83959-bound dopamine receptor 1 in complex with Gs protein
9LWC 2.9 Å, Cryo-EM structure of epinephrine bound dopamine receptor 1…
7JV5 3.0 Å, Cryo-EM structure of SKF-81297-bound dopamine receptor 1 in complex with Gs protein

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