9N09: Mini-Gs/i chimera
Human M4 muscarinic acetylcholine receptor Gi1 complex (mini-Gsi chimera) bound to the bitopic agonist C110. Determined by electron microscopy at 2.57 Å resolution. Released 28 Jan 2026.
- Method
- Electron microscopy
- Resolution
- 2.57 Å
- Organisms
- Homo sapiens, Lama glama
- Chains
- 5
- Atoms
- 7,958
- Mol. weight
- 144.4 kDa
- Ligands
- 5XI
- Released
- 28 Jan 2026
Explore 9N09 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9N09 contains 39 α-helices and 46 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 11 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-32 | 26 | |
| β-strand | 33-40 | 8 | 1 |
| α-helix | 42-44 | 3 | |
| α-helix | 46-48 | 3 | |
| β-strand | 168-174 | 7 | 1 |
| β-strand | 177-183 | 7 | 1 |
| α-helix | 194-198 | 5 | |
| β-strand | 203-209 | 7 | 1 |
| α-helix | 226-238 | 13 | |
| β-strand | 246-252 | 7 | 1 |
| α-helix | 254-263 | 10 | |
| α-helix | 268-270 | 3 | |
| α-helix | 273-275 | 3 | |
| α-helix | 286-287 | 2 | |
| α-helix | 292-311 | 20 | |
| β-strand | 319-323 | 5 | 1 |
| α-helix | 331-350 | 20 | |
Chain B: 3 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-25 | 23 | |
| α-helix | 30-33 | 4 | |
| α-helix | 34-36 | 3 | |
| β-strand | 47-52 | 6 | 2 |
| β-strand | 58-63 | 6 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 89-94 | 6 | 3 |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 121-125 | 5 | 4 |
| β-strand | 135-139 | 5 | 4 |
| β-strand | 146-151 | 6 | 5 |
| β-strand | 156-161 | 6 | 5 |
| β-strand | 165-170 | 6 | 5 |
| β-strand | 176-181 | 6 | 5 |
| β-strand | 187-192 | 6 | 6 |
| β-strand | 198-203 | 6 | 6 |
| β-strand | 208-212 | 5 | 6 |
| β-strand | 218-222 | 5 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 250-254 | 5 | 7 |
| β-strand | 259-264 | 6 | 7 |
| β-strand | 273-278 | 6 | 8 |
| β-strand | 284-289 | 6 | 8 |
| β-strand | 294-298 | 5 | 8 |
| β-strand | 304-308 | 5 | 8 |
| β-strand | 315-320 | 6 | 2 |
| β-strand | 327-331 | 5 | 2 |
| β-strand | 335-339 | 5 | 2 |
Chain G: 5 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-23 | 17 | |
| α-helix | 27-29 | 3 | |
| α-helix | 30-44 | 15 | |
| α-helix | 45-47 | 3 | |
| α-helix | 53-55 | 3 | |
Chain N: 3 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 9 |
| β-strand | 11-12 | 2 | 10 |
| β-strand | 18-25 | 8 | 9 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 11 |
| β-strand | 45-51 | 7 | 11 |
| β-strand | 58-60 | 3 | 11 |
| α-helix | 62-64 | 3 | |
| β-strand | 68-73 | 6 | 9 |
| β-strand | 78-83 | 6 | 9 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 11 |
| β-strand | 118 | 1 | 11 |
| β-strand | 122-124 | 3 | 11 |
| β-strand | 125-126 | 2 | 10 |
Chain R: 17 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 32-59 | 28 | |
| α-helix | 61-63 | 3 | |
| α-helix | 66-80 | 15 | |
| α-helix | 81-85 | 5 | |
| α-helix | 86-95 | 10 | |
| α-helix | 102-135 | 34 | |
| α-helix | 140-143 | 4 | |
| α-helix | 146-169 | 24 | |
| α-helix | 171-174 | 4 | |
| α-helix | 189-191 | 3 | |
| α-helix | 193-200 | 8 | |
| α-helix | 201-205 | 5 | |
| α-helix | 206-221 | 16 | |
| α-helix | 394-425 | 32 | |
| α-helix | 432-449 | 18 | |
| α-helix | 450-454 | 5 | |
| α-helix | 458-466 | 9 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| mini-Gs/i chimera | A | protein | 257 | Homo sapiens | |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 349 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 70 | Homo sapiens | P59768 (AlphaFold model) |
| Nb35 | N | protein | 128 | Lama glama | |
| Muscarinic acetylcholine receptor M4 | R | protein | 493 | Homo sapiens | P08173 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>9N09_1 mini-Gs/i chimera (chains A)
GGGSLEVLFQGPGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGADNSGKSTI
VKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFV
VDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFAR
YTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFN
DVTDIIIKMNLRDCGLF
Sequence of entity 2 (B), FASTA
>9N09_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTL
RGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNY
VACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDI
ETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINA
ICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDD
FNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>9N09_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFCAIL
Sequence of entity 4 (N), FASTA
>9N09_4 Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSS
Sequence of entity 5 (R), FASTA
>9N09_5 Muscarinic acetylcholine receptor M4 (chains R)
DYKDDDDANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVM
LSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYV
VSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFV
VGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGP
KEKKAKTLAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSN
ESSSGSATQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECV
TAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWT
PYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRN
IGTARHHHHHHHH
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| 5XI | methyl 4-[4-(3-methyl-2-oxidanylidene-benzimidazol-1-yl)piperidin-1-yl]piperidi… | C20 H28 N4 O3 | 1 |
Primary citation
Activation of the M4 muscarinic acetylcholine receptor by the bitopic agonist C110. Kaoullas, M.G., Mobbs, J.I., Venugopal, H. et al. To be published.
Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
- 8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
- 9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
- 6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
Browse structure collections
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