9N1Q: GCGR-Gs complex with SRB103H
cryo-EM structure of GCGR-Gs complex with SRB103H. Determined by electron microscopy at 3.0 Å resolution. Released 22 Apr 2026.
- Method
- Electron microscopy
- Resolution
- 3.0 Å
- Organisms
- Homo sapiens, Lama glama, synthetic construct
- Chains
- 6
- Atoms
- 9,339
- Mol. weight
- 162.91 kDa
- Released
- 22 Apr 2026
Explore 9N1Q in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9N1Q contains 38 α-helices and 53 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 9 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-37 | 28 | |
| β-strand | 40-45 | 6 | 1 |
| α-helix | 54-57 | 4 | |
| β-strand | 209-214 | 6 | 1 |
| β-strand | 217-223 | 7 | 1 |
| β-strand | 243-249 | 7 | 1 |
| α-helix | 265-277 | 13 | |
| β-strand | 286-292 | 7 | 1 |
| α-helix | 295-302 | 8 | |
| α-helix | 308-311 | 4 | |
| α-helix | 313-316 | 4 | |
| α-helix | 326-327 | 2 | |
| α-helix | 334-349 | 16 | |
| β-strand | 359-360 | 2 | 1 |
| α-helix | 371-390 | 20 | |
Chain B: 5 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-25 | 23 | |
| α-helix | 30-33 | 4 | |
| α-helix | 38-39 | 2 | |
| β-strand | 49-51 | 3 | 2 |
| β-strand | 58-62 | 5 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 89-94 | 6 | 3 |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 120-125 | 6 | 4 |
| β-strand | 134-140 | 7 | 4 |
| β-strand | 146-151 | 6 | 5 |
| β-strand | 157-161 | 5 | 5 |
| β-strand | 166-168 | 3 | 5 |
| β-strand | 178-180 | 3 | 5 |
| β-strand | 187-192 | 6 | 6 |
| β-strand | 198-203 | 6 | 6 |
| β-strand | 207-212 | 6 | 6 |
| β-strand | 218-222 | 5 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 250-254 | 5 | 7 |
| β-strand | 259-264 | 6 | 7 |
| α-helix | 272 | 1 | |
| β-strand | 273-278 | 6 | 8 |
| β-strand | 284-289 | 6 | 8 |
| β-strand | 294-298 | 5 | 8 |
| α-helix | 299-301 | 3 | |
| β-strand | 303-308 | 6 | 8 |
| β-strand | 315-320 | 6 | 2 |
| β-strand | 327-331 | 5 | 2 |
| β-strand | 336-339 | 4 | 2 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-20 | 13 | |
| α-helix | 30-43 | 14 | |
| α-helix | 45-47 | 3 | |
| α-helix | 53-55 | 3 | |
Chain N: 2 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 9 |
| β-strand | 10-12 | 3 | 10 |
| β-strand | 18-25 | 8 | 9 |
| β-strand | 34-39 | 6 | 10 |
| β-strand | 45-51 | 7 | 10 |
| β-strand | 58 | 1 | 10 |
| α-helix | 62-64 | 3 | |
| β-strand | 68-73 | 6 | 9 |
| β-strand | 78-83 | 6 | 9 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 10 |
| β-strand | 118 | 1 | 10 |
| β-strand | 122-126 | 5 | 10 |
Chain P: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-28 | 27 | |
Chain R: 17 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 28-48 | 21 | |
| α-helix | 51-53 | 3 | |
| β-strand | 58 | 1 | 11 |
| β-strand | 61-62 | 2 | 12 |
| β-strand | 67-68 | 2 | 12 |
| β-strand | 71 | 1 | 11 |
| β-strand | 76-80 | 5 | 13 |
| α-helix | 81-82 | 2 | |
| α-helix | 88-91 | 4 | |
| β-strand | 95-99 | 5 | 13 |
| β-strand | 100 | 1 | 14 |
| β-strand | 106 | 1 | 14 |
| α-helix | 107-108 | 2 | |
| α-helix | 125-164 | 40 | |
| α-helix | 172-201 | 30 | |
| α-helix | 205-206 | 2 | |
| α-helix | 209-215 | 7 | |
| α-helix | 218-253 | 36 | |
| α-helix | 264-268 | 5 | |
| α-helix | 269-273 | 5 | |
| α-helix | 276-286 | 11 | |
| α-helix | 309-335 | 27 | |
| α-helix | 347-358 | 12 | |
| α-helix | 376-399 | 24 | |
| α-helix | 405-421 | 17 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 394 | Homo sapiens | P63092 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 350 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 58 | Homo sapiens | P59768 (AlphaFold model) |
| Nb35 | N | protein | 128 | Lama glama | |
| SRB103H | P | protein | 37 | synthetic construct | |
| Glucagon receptor | R | protein | 475 | Homo sapiens | P47871 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>9N1Q_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
Sequence of entity 2 (B), FASTA
>9N1Q_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>9N1Q_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
NTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFR
Sequence of entity 4 (N), FASTA
>9N1Q_4 Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSS
Sequence of entity 5 (P), FASTA
>9N1Q_5 SRB103H (chains P)
HAHGTFTSDYSKYLDAKRAQEFIEWLLAGHHHHHPSW
Sequence of entity 6 (R), FASTA
>9N1Q_6 Glucagon receptor (chains R)
MKTIIALSYIFCLVFAAQVMDFLFEKWKLYGDQCHHNLSLLPPPTELVCNRTFDKYSCWP
DTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDGQWVRGPRGQPWRDASQCQMDGEEIE
VQKEVAKMYSSFQVMYTVGYSLSLGALLLALAILGGLSKLHCTRNAIHANLFASFVLKAS
SVLVIDGLLRTRYSQKIGDDLSVSTWLSDGAVAGCRVAAVFMQYGIVANYCWLLVEGLYL
HNLLGLATLPERSFFSLYLGIGWGAPMLFVVPWAVVKCLFENVQCWTSNDNMGFWWILRF
PVFLAILINFFIFVRIVQLLVAKLRARQMHHTDYKFRLAKSTLTLIPLLGVHEVVFAFVT
DEHAQGTLRSAKLFFDLFLSSFQGLLVAVLYCFLNKEVQSELRRRWHRWRLGKVLWEERN
TSNHRASSSPGHGPPSKELQFGRGGGSQDSSAETPLAGGLPRLAESPFDYKDDDD
Primary citation
cryo-EM structure of GCGR-Gs complex with SRB103H. Zhang, X., Mohebali, N., Belousoff, M.J. et al. To be published.
Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
- 6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
- 7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)
Browse structure collections
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