9ROL: PDB entry 9ROL

Human alpha3 Q140L Na+,K+-ATPase in the Na+-occluded E2P state. Determined by electron microscopy at 3.54 Å resolution. Released 12 Aug 2026.

Method: Electron microscopy
Resolution: 3.54 Å
Organism: Homo sapiens
Chains: 3
Atoms: 10,298
Mol. weight: 159.74 kDa
Ligands: NAG, CLR
Released: 12 Aug 2026

Explore 9ROL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9ROL contains 62 α-helices and 46 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 49 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	38-45	8
α-helix	55-65	11
α-helix	82-88	7
α-helix	91-111	21
α-helix	118-148	31
β-strand	158-163	6	1
β-strand	166-171	6	1
α-helix	172-174	3
β-strand	180-183	4	1
β-strand	187-189	3	1
β-strand	193-205	13	1
β-strand	211-216	6	1
α-helix	217	1
β-strand	232-233	2	1
β-strand	237-248	12	1
α-helix	252-254	3
α-helix	256-264	9
α-helix	273-302	30
α-helix	307-321	15
α-helix	326-343	18
β-strand	348	1	2
α-helix	353-358	6
β-strand	362-364	3	2
β-strand	372	1	3
β-strand	377-380	4	4
β-strand	381-383	3	5
β-strand	386-388	3	5
α-helix	406-418	13
α-helix	441-454	14
α-helix	458-463	6
β-strand	464-470	7	4
β-strand	479-485	7	4
β-strand	493-499	7	4
α-helix	501-506	6
β-strand	510-513	4	4
β-strand	516-519	4	4
α-helix	522-536	15
β-strand	542-549	8	4
β-strand	574-582	9	4
α-helix	584	1
β-strand	585	1	3
α-helix	586	1
α-helix	588-598	11
β-strand	602-604	3	2
α-helix	611-621	11
α-helix	631-638	8
α-helix	642-644	3
β-strand	653-656	4	6
α-helix	665-674	10
β-strand	678-681	4	6
α-helix	685-697	13
β-strand	702-706	5	2
α-helix	711-717	7
β-strand	720-724	5	2
α-helix	730-735	6
β-strand	738-740	3	2
α-helix	746-769	24
α-helix	770-773	4
α-helix	778-786	9
α-helix	794-804	11
α-helix	807-811	5
α-helix	812-814	3
α-helix	815-818	4
α-helix	821-823	3
α-helix	825-827	3
α-helix	837-841	5
α-helix	842-846	5
α-helix	847-866	20
α-helix	870-872	3
α-helix	877-881	5
β-strand	888-889	2	7
β-strand	895-896	2	7
α-helix	898-926	29
α-helix	934-937	4
α-helix	942-960	19
α-helix	962-965	4
α-helix	975-979	5
α-helix	982-1001	20
α-helix	1006-1011	6

Chain B: 12 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	29-60	32
β-strand	76	1	8
β-strand	78-79	2	9
β-strand	87-90	4	10
α-helix	95-97	3
α-helix	99-109	11
α-helix	110-112	3
α-helix	114-116	3
β-strand	124	1	11
α-helix	132-135	4
β-strand	149	1	11
α-helix	153-155	3
β-strand	175-179	5	9
α-helix	180-182	3
β-strand	184	1	12
α-helix	188-190	3
α-helix	199-201	3
α-helix	205-207	3
β-strand	209-210	2	13
β-strand	211-214	4	10
β-strand	228-230	3	9
α-helix	232-234	3
β-strand	237-238	2	13
β-strand	245	1	12
β-strand	259-262	4	9
β-strand	272-278	7	10
β-strand	292	1	8
β-strand	294-301	8	10

Chain C: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	33-57	25

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Sodium/potassium-transporting ATPase subunit alpha-3	A	protein	1013	Homo sapiens	P13637 (AlphaFold model)
Sodium/potassium-transporting ATPase subunit beta-1	B	protein	319	Homo sapiens	P05026 (AlphaFold model)
Phospholemman	C	protein	76	Homo sapiens	O00168 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9ROL_1 Sodium/potassium-transporting ATPase subunit alpha-3 (chains A)
MGDKKDDKDSPKKNKGKERRDLDDLKKEVAMTEHKMSVEEVCRKYNTDCVQGLTHSKAQE
ILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCFLAYGIQAGTEDDPSGDNL
YLGIVLAAVVIITGCFSYYLEAKSSKIMESFKNMVPQQALVIREGEKMQVNAEEVVVGDL
VEIKGGDRVPADLRIISAHGCKVDNSSLTGESEPQTRSPDCTHDNPLETRNITFFSTNCV
EGTARGVVVATGDRTVMGRIATLASGLEVGKTPIAIEIEHFIQLITGVAVFLGVSFFILS
LILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGST
STICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTSFDKSSHTWVALSHIAGLCNR
AVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRERNKKVAEIPFNSTNKYQL
SIHETEDPNDNRYLLVMKGAPERILDRCSTILLQGKEQPLDEEMKEAFQNAYLELGGLGE
RVLGFCHYYLPEEQFPKGFAFDCDDVNFTTDNLCFVGLMSMIDPPRAAVPDAVGKCRSAG
IKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVIHGTDL
KDFTSEQIDEILQNHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADI
GVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPF
LLFIMANIPLPLGTITILCIDLGTDMVPAISLAYEAAESDIMKRQPRNPRTDKLVNERLI
SMAYGQIGMIQALGGFFSYFVILAENGFLPGNLVGIRLNWDDRTVNDLEDSYGQQWTYEQ
RKVVEFTCHTAFFVSIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSY
CPGMDVALRMYPLKPSWWFCAFPYSFLIFVYDEIRKLILRRNPGGWVEKETYY

Sequence of entity 2 (B), FASTA

>9ROL_2 Sodium/potassium-transporting ATPase subunit beta-1 (chains B)
MARSHHHHHHHHHHPRRSRGKAKEEGSWKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYG
CLAGIFIGTIQVMLLTISEFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLN
IVRFLEKYKDSAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLND
ETYGYKEGKPCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKV
GNVEYFGLGNSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIG
YSEKDRFQGRFDVKIEVKS

Sequence of entity 3 (C), FASTA

>9ROL_3 Phospholemman (chains C)
GTKAESPKEHDPFTYDYQSLQIGGLVIAGILFILGILIVLSRRCRCKFNQQQRTGEPDEE
EGTFRSSIRRLSTRRR

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	3
CLR	Cholesterol	C27 H46 O	3

Primary citation

Active conformations of neuronal Na + , K + -ATPase isoforms and a disease-causing mutant. Christensen, M.E., Habeck, M., Katz, A. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-75997-4 · PubMed

Other PDB entries of the same protein (UniProt P13637 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9ROG 2.8 Å, Human alpha3 Na+,K+-ATPase in the Na+-occluded E1P-ADP state obtained under turnover…
9ROH 2.92 Å, Human alpha3 Na+,K+-ATPase in the Na+-occluded E2P state obtained under turnover…
9ROE 2.94 Å, Human alpha3 Na+,K+-ATPase in the outward open E2P state
9ROI 2.94 Å, Human alpha3 Na+,K+-ATPase in the K+-occluded E2P state obtained under turnover conditions
9ROA 3.08 Å, Human alpha3 Na+,K+-ATPase in the Na+-occluded E1P-ADP state
9ROD 3.12 Å, Human alpha3 Na+,K+-ATPase in the Na+-occluded E2P state
9RO9 3.27 Å, Human alpha3 Na+,K+-ATPase in the Na+-bound E1-ATP state
9ROF 3.27 Å, Human alpha3 Na+,K+-ATPase in the Na+-bound E1-ATP state obtained under turnover…
8D3V 3.4 Å, Human alpha3 Na+/K+-ATPase in its cytoplasmic side-open state
8D3W 3.5 Å, Human alpha3 Na+/K+-ATPase in its AMPPCP-bound cytoplasmic side-open state
9ROJ 3.58 Å, Human alpha3 Q140L Na+,K+-ATPase in the Na+-bound E1 state
9ROM 3.6 Å, Human alpha3 Q140L Na+,K+-ATPase in the outward open E2P state

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