9UY3: Engineered G-alpha-q subunit
Anamorelin bound growth hormone secretagogue receptor in complex with Gq. Determined by electron microscopy at 2.52 Å resolution. Released 2 Jul 2025.
- Method
- Electron microscopy
- Resolution
- 2.52 Å
- Organisms
- Homo sapiens, Lama glama, Escherichia coli
- Chains
- 5
- Atoms
- 8,245
- Mol. weight
- 182.76 kDa
- Ligands
- A1ESD, CLR
- Released
- 2 Jul 2025
Explore 9UY3 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9UY3 contains 34 α-helices and 48 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 16-39 | 24 | |
| β-strand | 40-47 | 8 | 1 |
| α-helix | 53-59 | 7 | |
| β-strand | 207-214 | 8 | 1 |
| β-strand | 217-224 | 8 | 1 |
| α-helix | 234-238 | 5 | |
| β-strand | 243-249 | 7 | 1 |
| α-helix | 266-278 | 13 | |
| α-helix | 280-282 | 3 | |
| β-strand | 286-292 | 7 | 1 |
| α-helix | 294-303 | 10 | |
| α-helix | 308-310 | 3 | |
| α-helix | 313-316 | 4 | |
| α-helix | 332-351 | 20 | |
| β-strand | 359-363 | 5 | 1 |
| α-helix | 371-390 | 20 | |
Chain B: 4 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-24 | 21 | |
| α-helix | 30-34 | 5 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 2 |
| β-strand | 58-63 | 6 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 88-94 | 7 | 3 |
| α-helix | 95 | 1 | |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 121-125 | 5 | 4 |
| β-strand | 134-139 | 6 | 4 |
| β-strand | 146-151 | 6 | 5 |
| β-strand | 156-161 | 6 | 5 |
| β-strand | 166-170 | 5 | 5 |
| β-strand | 175-180 | 6 | 5 |
| β-strand | 189-192 | 4 | 6 |
| β-strand | 198-202 | 5 | 6 |
| β-strand | 207-212 | 6 | 6 |
| β-strand | 217-223 | 7 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 250-254 | 5 | 7 |
| β-strand | 259-264 | 6 | 7 |
| β-strand | 273-278 | 6 | 8 |
| β-strand | 284-289 | 6 | 8 |
| β-strand | 294-298 | 5 | 8 |
| β-strand | 304-308 | 5 | 8 |
| β-strand | 315-320 | 6 | 2 |
| β-strand | 327-331 | 5 | 2 |
| β-strand | 336-339 | 4 | 2 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-23 | 16 | |
| α-helix | 30-44 | 15 | |
| α-helix | 45-47 | 3 | |
| α-helix | 53-55 | 3 | |
Chain N: 2 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 9 |
| β-strand | 11-12 | 2 | 10 |
| β-strand | 18-25 | 8 | 9 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 11 |
| β-strand | 45-51 | 7 | 11 |
| β-strand | 58-60 | 3 | 11 |
| β-strand | 68-73 | 6 | 9 |
| β-strand | 78-83 | 6 | 9 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 11 |
| β-strand | 118 | 1 | 11 |
| β-strand | 122-124 | 3 | 11 |
| β-strand | 125-126 | 2 | 10 |
Chain R: 14 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 40-70 | 31 | |
| α-helix | 72-74 | 3 | |
| α-helix | 77-100 | 24 | |
| α-helix | 101-105 | 5 | |
| α-helix | 113-142 | 30 | |
| α-helix | 148-154 | 7 | |
| α-helix | 157-175 | 19 | |
| α-helix | 178-181 | 4 | |
| β-strand | 182-185 | 4 | 12 |
| β-strand | 197-200 | 4 | 12 |
| α-helix | 202-207 | 6 | |
| α-helix | 209-240 | 32 | |
| α-helix | 258-288 | 31 | |
| α-helix | 295-320 | 26 | |
| α-helix | 321-325 | 5 | |
| α-helix | 328-333 | 6 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Engineered G-alpha-q subunit | A | protein | 362 | Homo sapiens | |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 371 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| NB35 | N | protein | 140 | Lama glama | |
| Soluble cytochrome b562,Growth hormone secretagogue receptor type 1,Soluble cytochrome b562,Growth… | R | protein | 676 | Escherichia coli, Homo sapiens | Q92847 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>9UY3_1 Engineered G-alpha-q subunit (chains A)
MMGCTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVN
GYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFM
TAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRV
KTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALND
FKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDP
RVTRAKYFIRKEFVDISTASGDGRHICYPHFTCSVDTENARRIFNDCKDIILQMNLREYN
LV
Sequence of entity 2 (B), FASTA
>9UY3_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSG
VSGWRLFKKIS
Sequence of entity 3 (G), FASTA
>9UY3_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 4 (N), FASTA
>9UY3_4 NB35 (chains N)
MAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASI
SYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYR
GQGTQVTVSSHHHHHHEPEA
Sequence of entity 5 (R), FASTA
>9UY3_5 Soluble cytochrome b562,Growth hormone secretagogue receptor type 1,Soluble cytochrome b562,Growth hormone secretagogue receptor type 1,Soluble cytochrome b562,Growth hormone secretagogue receptor type 1,Soluble cytochrome b562,Growth hormone secretagogue receptor type 1,human growth hormone secretagogue receptor (chains R)
MKTIIALSYIFCLVFAHHHHHHHHHHPSRLEEELRRRLTEPADLEDNWETLNDNLKVIEK
ADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLA
NEGKVKEAQAAAEQLKTTRNAYIQKYLMWNATPSEEPGFNLTLADLDWDASPGNDSLGDE
LLQLFPAPLLAGVTATCVALFVVGIAGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIF
LCMPLDLVRLWQYRPWNFGDLLCKLFQFVSESCTYATVLTITALSVERYFAICFPLRAKV
VVTKGRVKLVIFVIWAVAFCSAGPIFVLVGVEHENGTDPWDTNECRPTEFAVRSGLLTVM
VWVSSIFFFLPVFCLTVLYSLIGRKLWRRRRGDAVVGASLRDQNHKQTVKMLAVVVFAFI
LCWLPFHVGRYLFSKSFEPGSLEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVA
VFRLLGFEPFSQRKLSTLKDESSRAWTESSINTGSAGSVFTLEDFVGDWEQTAAYNLDQV
LEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVY
PVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDER
LITPDGSMLFRVTINS
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| A1ESD | 2-azanyl-N-[(2R)-1-[(3S)-3-[dimethylamino(methyl)carbamoyl]-3-(phenylmethyl)pip… | C31 H42 N6 O3 | 1 |
| CLR | Cholesterol | C27 H46 O | 2 |
Primary citation
Molecular recognition of two approved drugs Macimorelin and Anamorelin by the growth hormone secretagogue receptor. Wang, R.L., Sun, J., Liu, H. et al. Acta Pharmacol Sin (2025) 46:2998-3008. DOI 10.1038/s41401-025-01606-7 · PubMed
Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
- 8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
- 9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
- 6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
Browse structure collections
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