9V0X: Gq-coupled PrRPR
Cryo-EM structure of Gq-coupled PrRPR in complex with GUB08248. Determined by electron microscopy at 2.66 Å resolution. Released 4 Feb 2026.
- Method
- Electron microscopy
- Resolution
- 2.66 Å
- Organism
- Homo sapiens
- Chains
- 6
- Atoms
- 9,126
- Mol. weight
- 159.06 kDa
- Ligands
- KUT
- Released
- 4 Feb 2026
Explore 9V0X in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9V0X contains 37 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-32 | 24 | |
| β-strand | 34-40 | 7 | 1 |
| α-helix | 46-51 | 6 | |
| β-strand | 184-191 | 8 | 1 |
| β-strand | 194-201 | 8 | 1 |
| α-helix | 211-213 | 3 | |
| β-strand | 220-226 | 7 | 1 |
| α-helix | 233-244 | 12 | |
| α-helix | 247-249 | 3 | |
| β-strand | 254-259 | 6 | 1 |
| α-helix | 261-270 | 10 | |
| α-helix | 275-278 | 4 | |
| α-helix | 280-283 | 4 | |
| α-helix | 299-318 | 20 | |
| β-strand | 326-330 | 5 | 1 |
| α-helix | 338-357 | 20 | |
Chain B: 5 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 12-29 | 18 | |
| α-helix | 35-39 | 5 | |
| α-helix | 43-44 | 2 | |
| β-strand | 52-56 | 5 | 2 |
| α-helix | 62 | 1 | |
| β-strand | 63-68 | 6 | 3 |
| β-strand | 74-79 | 6 | 3 |
| β-strand | 83-88 | 6 | 3 |
| β-strand | 94-99 | 6 | 3 |
| β-strand | 105-110 | 6 | 4 |
| β-strand | 116-121 | 6 | 4 |
| β-strand | 126-130 | 5 | 4 |
| β-strand | 139-144 | 6 | 4 |
| β-strand | 151-156 | 6 | 5 |
| β-strand | 161-166 | 6 | 5 |
| β-strand | 170-175 | 6 | 5 |
| β-strand | 180-186 | 7 | 5 |
| β-strand | 192-197 | 6 | 6 |
| β-strand | 203-208 | 6 | 6 |
| β-strand | 213-217 | 5 | 6 |
| β-strand | 223-227 | 5 | 6 |
| β-strand | 234-239 | 6 | 7 |
| β-strand | 245-250 | 6 | 7 |
| β-strand | 255-259 | 5 | 7 |
| β-strand | 264-269 | 6 | 7 |
| β-strand | 278-283 | 6 | 8 |
| β-strand | 289-294 | 6 | 8 |
| β-strand | 299-303 | 5 | 8 |
| β-strand | 309-312 | 4 | 8 |
| α-helix | 313-315 | 3 | |
| β-strand | 320-325 | 6 | 2 |
| β-strand | 332-336 | 5 | 2 |
| β-strand | 341-344 | 4 | 2 |
Chain E: 5 helices, 25 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-6 | 5 | 9 |
| β-strand | 9-11 | 3 | 10 |
| β-strand | 17-24 | 8 | 9 |
| β-strand | 32-38 | 7 | 10 |
| β-strand | 44-50 | 7 | 10 |
| α-helix | 52-54 | 3 | |
| β-strand | 57-59 | 3 | 10 |
| α-helix | 61-63 | 3 | |
| β-strand | 67-72 | 6 | 9 |
| β-strand | 77-82 | 6 | 9 |
| α-helix | 87-89 | 3 | |
| β-strand | 91-98 | 8 | 10 |
| β-strand | 109-110 | 2 | 10 |
| β-strand | 114-118 | 5 | 10 |
| β-strand | 140-141 | 2 | 11 |
| β-strand | 146-148 | 3 | 12 |
| β-strand | 155-161 | 7 | 11 |
| β-strand | 166 | 1 | 13 |
| β-strand | 172 | 1 | 13 |
| β-strand | 174-179 | 6 | 12 |
| β-strand | 186-189 | 4 | 12 |
| β-strand | 191 | 1 | 14 |
| β-strand | 194 | 1 | 14 |
| β-strand | 203-207 | 5 | 11 |
| β-strand | 211-216 | 6 | 11 |
| α-helix | 221-223 | 3 | |
| β-strand | 225-231 | 7 | 12 |
| α-helix | 237 | 1 | |
| β-strand | 239 | 1 | 12 |
| β-strand | 243-246 | 4 | 12 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-23 | 14 | |
| α-helix | 29-42 | 14 | |
| α-helix | 44-46 | 3 | |
| α-helix | 52-54 | 3 | |
Chain R: 13 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 55 | 1 | 15 |
| β-strand | 58 | 1 | 15 |
| α-helix | 60-85 | 26 | |
| α-helix | 89-91 | 3 | |
| α-helix | 94-121 | 28 | |
| α-helix | 132-163 | 32 | |
| α-helix | 173-190 | 18 | |
| α-helix | 192-195 | 4 | |
| β-strand | 199 | 1 | 16 |
| α-helix | 204-206 | 3 | |
| β-strand | 212 | 1 | 16 |
| α-helix | 220-230 | 11 | |
| α-helix | 235-254 | 20 | |
| α-helix | 269-303 | 35 | |
| α-helix | 310-332 | 23 | |
| α-helix | 333-337 | 5 | |
| α-helix | 340-350 | 11 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(324) subunit alpha | A | protein | 361 | Homo sapiens | |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 371 | Homo sapiens | P62873 (AlphaFold model) |
| scFv16 | E | protein | 247 | Homo sapiens | |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 70 | Homo sapiens | P59768 (AlphaFold model) |
| GUB08248 | L | protein | 11 | Homo sapiens | |
| Prolactin-releasing peptide receptor | R | protein | 370 | Homo sapiens | P49683 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>9V0X_1 Guanine nucleotide-binding protein G(324) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNLREYNL
V
Sequence of entity 2 (B), FASTA
>9V0X_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSG
VSGWRLFKKIS
Sequence of entity 3 (E), FASTA
>9V0X_3 scFv16 (chains E)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL
Sequence of entity 4 (G), FASTA
>9V0X_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFCAIL
Sequence of entity 5 (L), FASTA
>9V0X_5 GUB08248 (chains L)
SRGKRPVGRFA
Sequence of entity 6 (R), FASTA
>9V0X_6 Prolactin-releasing peptide receptor (chains R)
MASSTTRGPRVSDLFSGLPPAVTTPANQSAEASAGNGSVAGADAPAVTPFQSLQLVHQLK
GLIVLLYSVVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCTACVPLTLAY
AFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIAVDRYVVLVHPLRRRISLRLSAYAV
LAIWALSAVLALPAAVHTYHVELKPHDVRLCEEFWGSQERQRQLYAWGLLLVTYLLPLLV
ILLSYVRVSVKLRNRVVPGCVTQSQADWDRARRRRTFCLLVVIVVVFAVCWLPLHVFNLL
RDLDPHAIDPYAFGLVQLLCHWLAMSSACYNPFIYAWLHDSFREELRKLLVAWPRKIAPH
GQNMTVSVVI
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| KUT | KUT [2-(2-[2-(2-[2-(Octadecandioyl-gamma-Glu)amino]ethoxy)ethoxy]acetylamino)et… | C35 H63 N3 O12 | 1 |
Primary citation
Molecular basis for cross-activation of NPFF2R by a short PrRP-related peptide. Li, X., Li, S., Shan, H. et al. Acta Pharmacol Sin (2026) 47:1671-1681. DOI 10.1038/s41401-025-01741-1 · PubMed
Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
- 8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
- 9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
- 6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
Browse structure collections
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