9V80: KICSTOR CCC complex

Cryo-EM structure of KICSTOR CCC complex (state 4). Determined by electron microscopy at 2.95 Å resolution. Released 12 Nov 2025.

Method: Electron microscopy
Resolution: 2.95 Å
Organism: Homo sapiens
Chains: 3
Atoms: 9,607
Mol. weight: 475.95 kDa
Released: 12 Nov 2025

Explore 9V80 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9V80 contains 33 α-helices and 86 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	2234-2244	11
α-helix	2367-2390	24
α-helix	2503-2509	7
α-helix	2521-2525	5
α-helix	2526-2532	7
β-strand	2541	1	1
α-helix	2555-2568	14
β-strand	2575	1	2
β-strand	2578-2579	2	3
α-helix	2580	1
β-strand	2588-2589	2	3
β-strand	2607-2612	6	2
β-strand	2649	1	1
β-strand	2650-2655	6	2
β-strand	2658-2661	4	2
β-strand	2664	1	1
α-helix	2668-2687	20
α-helix	2691	1
α-helix	2692-2696	5
α-helix	2697-2699	3
α-helix	2729-2732	4
α-helix	2764-2766	3
α-helix	2790-2821	32
α-helix	2834-2842	9
β-strand	2845-2853	9	4
α-helix	2858-2860	3
α-helix	2909-2926	18
β-strand	2930-2931	2	4
α-helix	2933-2936	4
β-strand	2981-2986	6	4
β-strand	2990-2999	10	4
β-strand	3002-3011	10	4
α-helix	3027-3037	11
α-helix	3042-3054	13
α-helix	3071-3081	11
β-strand	3093-3095	3	5
β-strand	3124	1	5
β-strand	3140-3145	6	5
β-strand	3165-3170	6	5
β-strand	3190-3194	5	5
α-helix	3249-3253	5

Chain B: 4 helices, 35 β-strands

Element	Residues	Length	Sheet
β-strand	2-3	2	6
β-strand	9-13	5	7
β-strand	23-26	4	8
β-strand	35-40	6	8
β-strand	44-48	5	8
β-strand	57-59	3	8
β-strand	65-71	7	9
β-strand	80-85	6	9
β-strand	90-93	4	9
β-strand	119-120	2	9
β-strand	127	1	10
β-strand	131-133	3	11
β-strand	142-146	5	11
β-strand	147	1	10
β-strand	151-158	8	11
β-strand	170-178	9	11
α-helix	182-183	2
β-strand	186-187	2	12
β-strand	190	1	13
β-strand	196	1	13
β-strand	199-202	4	12
β-strand	206-209	4	12
β-strand	241-244	4	12
β-strand	256-260	5	14
β-strand	275-280	6	14
β-strand	284-289	6	14
β-strand	296-302	7	14
β-strand	307	1	15
β-strand	311-313	3	6
β-strand	322-325	4	6
β-strand	327	1	15
β-strand	331-335	5	6
β-strand	341-345	5	6
β-strand	350-359	10	7
β-strand	365-373	9	7
β-strand	377-381	5	7
α-helix	394-400	7
α-helix	403-409	7
α-helix	418-430	13

Chain C: 7 helices, 32 β-strands

Element	Residues	Length	Sheet
β-strand	14-21	8	16
β-strand	31-32	2	17
β-strand	41-44	4	17
β-strand	50-58	9	17
β-strand	61-69	9	17
β-strand	79-87	9	18
β-strand	95-103	9	18
β-strand	110-116	7	18
α-helix	127-130	4
β-strand	136-138	3	18
β-strand	146-153	8	19
β-strand	156-164	9	19
β-strand	170-176	7	19
β-strand	181-185	5	19
α-helix	192-194	3
β-strand	201-208	8	20
β-strand	214-221	8	20
β-strand	225-232	8	20
β-strand	238-245	8	20
β-strand	250-256	7	21
β-strand	274-279	6	21
β-strand	285-288	4	21
α-helix	290-293	4
β-strand	297-300	4	21
α-helix	301	1
β-strand	310-316	7	22
β-strand	325-330	6	22
β-strand	334	1	23
β-strand	335-341	7	22
α-helix	343-345	3
β-strand	353-358	6	22
β-strand	361	1	23
β-strand	366-369	4	16
β-strand	372	1	24
α-helix	380	1
β-strand	381	1	24
β-strand	384-386	3	16
β-strand	389-395	7	16
α-helix	397-418	22

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
KICSTOR complex protein SZT2	A	protein	3432	Homo sapiens	Q5T011 (AlphaFold model)
KICSTOR complex protein ITFG2	B	protein	447	Homo sapiens	Q969R8 (AlphaFold model)
KICSTOR complex protein kaptin	C	protein	436	Homo sapiens	Q9Y664 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9V80_1 KICSTOR complex protein SZT2 (chains A)
MASERPEPEVEEAGQVFLLMKKDYRISRNVRLAWFLSHLHQTVQATPQEMLLQSEQELEV
LSVLPPGWQPDEPVVPRPFLLVPSTRVTFLAWQYRFVIELDLSPSTGIVDDSTGEILFDE
VFHALSRCLGGLLRPFRVPGSCIDFQPEIYVTIQAYSSIIGLQSHQVLVQGCLLDPSQRE
VFLQQIYEQLCLFEDKVATMLQQQYDPQSQAEDQSPDSGDLLGRKVGVSMVTADLGLVSM
IRQGILALQLLPSNSSAGIIVITDGVTSVPDVAVCETLLNQLRSGTVACSFVQVGGVYSY
DCSFGHVPNVELMKFIAMATFGSYLSTCPEPEPGNLGLTVYHRAFLLYSFLRSGEALNPE
YYCGSQHRLFNEHLVSASSNPALALRRKKHTEKEVPADLVSTVSVRLREGYSVREVTLAK
GGSQLEVKLVLLWKHNMRIEYVAMAPWPLEPEGPRVTRVEVTMEGGYDILHDVSCALRQP
IRSLYRTHVIRRFWNTLQSINQTDQMLAHLQSFSSVPEHFTLPDSTKSGVPLFYIPPGST
TPVLSLQPSGSDSSHAQFAAYWKPVLSMDANSWQRWLHMHRLVLILEHDTPIPKHLHTPG
SNGRYSTIQCRISHSSLTSLLRDWSSFVLVEGYSYVKLLSSAPDQPPNSFYMVRIISKAP
CMVLRLGFPIGTPAPARHKIVSGLREEILRLRFPHRVQSKEPTPKVKRKGLGGAGGGSSP
SKSPPVLGPQQALSDRPCLVVLHKPLDKLLIRYEKLPLDYRAPFLLTLEPPGPLPLVSGR
SASSSLASLSRYLYHQRWLWSVPSGLAPALPLSAIAQLLSILTEVRLSEGFHFACSGEGI
INMVLELPIQNEPPGQAAAEEKHTCVVQYILFPPHSTSTKDSFSTDDDNDVEVEALEGDS
ELNLVTEVWVEPQYGRVGPGPGIWKHLQDLTYSEIPQALHPRDAACIGSMLSFEYLIQLC
QSKEWGPLPPEPRVSDGLDQGGDTCVHEIPFHFDLMGLLPQCQQLQMFFLLLAREPEGVP
FAEGSCPANDMVLCLLHSCLGQELSDREIPLTPVDQAAFLSEVLRRTCHVPGAEGPLLGV
HGIPKEQAVGSTQATGDSAFTSLSVGLPETLKPLISAQPPQWRCYARLVNPQHVFLTFLP
ATFSDVQRLAACGLEGPPQEETKPKFGDWSGAPSLKDLGGTGIKATKSHVPVLSVTLASD
NAQNQGELSPPFRRDLQAYAGRQASQTESADGPRTRCPVYIYSCSLEALREQMVGMQPPQ
APRDLIFRTQFLDHPSPSSAWMEPRYKEAANHCALLQEHAQRCYVRGLFRSLQQAQSVTS
QDLLTAVDACEELLQEIDITPFLLALCGHTWGLPHAPPSPGPLSPGPFSSSMEEGAEPRE
RAILASESSIETEDLSEPEFQSTRVPGIPDPGPEISLTDVCQLRGEAHGALHSVIQEKFL
EISRLHFRTVPSNPHYFFYCPPSSRREDEGPRDTVDRKISDLEFSEAELMGEEGDTSACC
VVTESDPELEVEYRESRESDLGPAGLDSASLSDVDTVNPDEDSFSILGGDSPTGPESFLH
DLPPLFLHLTCSVRLRGQHSSVPVCSLPTCLGQVLSSLEGPPVGGRVPLRDLSVTLDVFM
LTLPLEVELPTASDPQHHRSTSESSASFPRSPGQPSSLRSDDGLGPPLPPPEEERHPGLS
NLATPHRLAIETTMNEIRWLLEDEMVGALRRGGIPQSPALHRAAAHIHSSPGRSTCLRQT
LPLSFVFGPERSLTQFKEEFRRLHLPGHVLLEDPDSGFFFVAAGQQPGGSHGEPSSAAWA
WHSHEDRAEGIEGETLTASPQAPGSPEDSEGVPLISLPRVPQGGSQPGPSRGLSLMSSQG
SVDSDHLGYDGGSSGSDSEGPNDTLGEKAPFTLRTPPGPAPPQPSLSGLPGPCLPDFWLI
VRVLQDRVEVYAHARSLIREDGGPGTECRHLQQLLVRRVGEICREVNQRLLLQDLHDSHV
CNSLLVAESEEDLWRSETPFHSRQRAPLPSDDYAADESCAPRGYLAATMQFVPGHFSCDV
VWGTVIRVHSRLKMGPSMGVSRAIQALRSVLNAFSVVNRKNMFVYQERATKAVYYLRLLE
TSCSDRPWKGDALPPSLALSRSQEPIYSEEASGPRSPLDMVSSRSSDAARPVGQVDRHIQ
LLVHGVGQAGPEITDELVRVLCRRLDEATLDVITVMLVRNCKLTPADVEFIQPPGSLPSE
VLHLALPTSCRPWLPALAWYLRQNLLIFLHSPKYTDSNSRNHFQHPLPPQGGLPDLDIYL
YNKPGGQGTGGKGVACITLAFVDEGGAPLSLALWPPSSPGPPDPLREEEFEQLTQVIRCP
VVVDSSSAQNGAPRLRLDVWEKGNISIVQLEEKLRGAARQALADAIIELQLLPASLCTED
TPTGSLRNGSLETKSSAGRASTFPPAPVPGEPVTPPSKAGRRSFWDMLSKTECGDLGSPK
TTDDIVLDRPEDTRGRRRHKTESVRTPGGAERAPGSDSGAQRQKRRTTQLEEGEVGTLHP
VFARVAQRWMEFMVQIGCASVSRSSAHMVSRFLLPSILSEFTALVTSMAGDTSVRIFEQH
LGSEPEIFGPCSPGQLGPSPRPAAERHLLLLGRNFLQWRRPTQQAAKAMQRFEPGGDGSS
GRNAPRQRLLLLEVVDKKLQLLTYNWAPDLGAALGRALVRLVQWQNARAHLIFCLLSQKL
GLFHHYGQLDFPVRDEKEPNPFLLPTMEVETLIRSASPPLSREQGRLSGSSRGGGPLPLD
TFPFDEALRDITAARPSSVLGPVPRPPDPVTYHGQQFLEIKMAERRELERQMKMENLFVT
WQQRSTPATMPISAGELETLKQSSRLVHYCATAMLFDPAAWLHGPPETSGPPDGQRRHRP
ESGSGSREAPTSCESLDVSPPGAREEPWLKELSLAFLQQYVQYLQSIGFVLVPLRPPSPA
RSTSRPRAMAILGTEGRGSFSCPKTKTDGSPKSTSSPVTTYHLQRALPGGIILMELAFQG
CYFCVKQFALECSRIPMGQAVNSQLSMLFTEECDKVRDLMHVHSFSYDFHLRLVHQHVLG
AHLVLRHGYHLTTFLRHFLAHHPDGPHFGRNHIYQGTLELPTPLIAAHQLYNYVADHASS
YHMKPLRMARPGGPEHNEYALVSAWHSSGSYLDSEGLRHQDDFDVSLLVCHCAAPFEEQG
EAERHVLRLQFFVVLTSQRELFPRLTADMRRFRKPPRLPPEPEAPGSSAGSPGEASGLIL
APGPAPLFPPLAAEVGMARARLAQLVRLAGGHCRRDTLWKRLFLLEPPGPDRLRLGGRLA
LAELEELLEAVHAKSIGDIDPQLDCFLSMTVSWYQSLIKVLLSRFPQSCRHFQSPDLGTQ
YLVVLNQKFTDCFVLVFLDSHLGKTSLTVVFREPFPVQPQDSESPPAQLVSTYHHLESVI
NTACFTLWTRLL

Sequence of entity 2 (B), FASTA

>9V80_2 KICSTOR complex protein ITFG2 (chains B)
MRSVSYVQRVALEFSGSLFPHAICLGDVDNDTLNELVVGDTSGKVSVYKNDDSRPWLTCS
CQGMLTCVGVGDVCNKGKNLLVAVSAEGWFHLFDLTPAKVLDASGHHETLIGEEQRPVFK
QHIPANTKVMLISDIDGDGCRELVVGYTDRVVRAFRWEELGEGPEHLTGQLVSLKKWMLE
GQVDSLSVTLGPLGLPELMVSQPGCAYAILLCTWKKDTGSPPASEGPTDGSRETPAARDV
VLHQTSGRIHNKNVSTHLIGNIKQGHGTESSGSGLFALCTLDGTLKLMEEMEEADKLLWS
VQVDHQLFALEKLDVTGNGHEEVVACAWDGQTYIIDHNRTVVRFQVDENIRAFCAGLYAC
KEGRNSPCLVYVTFNQKIYVYWEVQLERMESTNLVKLLETKPEYHSLLQELGVDPDDLPV
TRALLHQTLYHPDQPPQCAPSSLQDPT

Sequence of entity 3 (C), FASTA

>9V80_3 KICSTOR complex protein kaptin (chains C)
MMGEAAVAAGPCPLREDSFTRFSSQSNVYGLAGGAGGRGELLAATLKGKVLGFRYQDLRQ
KIRPVAKELQFNYIPVDAEIVSIDTFNKSPPKRGLVVGITFIKDSGDKGSPFLNIYCDYE
PGSEYNLDSIAQSCLNLELQFTPFQLCHAEVQVGDQLETVFLLSGNDPAIHLYKENEGLH
QFEEQPVENLFPELTNLTSSVLWLDVHNFPGTSRRLSALGCQSGYVRVAHVDQRSREVLQ
MWSVLQDGPISRVIVFSLSAAKETKDRPLQDEYSVLVASMLEPAVVYRDLLNRGLEDQLL
LPGSDQFDSVLCSLVTDVDLDGRPEVLVATYGQELLCYKYRGPESGLPEAQHGFHLLWQR
SFSSPLLAMAHVDLTGDGLQELAVVSLKGVHILQHSLIQASELVLTRLRHQVEQRRRRLQ
GLEDGAGAGPAENAAS

Primary citation

Architecture of the human KICSTOR and GATOR1-KICSTOR complexes. Teng, F., Zeng, H., Mai, X. et al. Nat Struct Mol Biol (2025) 32:2587-2600. DOI 10.1038/s41594-025-01693-4 · PubMed

Other PDB entries of the same protein (UniProt Q5T011 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9VAN 2.9 Å, Cryo-EM structure of human full length KICSTOR complex (state 2)
9V0J 2.97 Å, Cryo-EM structure of GATOR1-KICSTOR complex
9V86 3.04 Å, Cryo-EM structure of KICSTOR CCC complex (state 3)
9V9N 3.08 Å, Cryo-EM structure of KICSTOR CCC complex (state 5)
9V6E 3.19 Å, Cryo-EM structure of human full length KICSTOR complex (state 1)
9O5A 3.2 Å, The KICSTOR-GATOR1 complex
9O5D 3.34 Å, The KICSTOR-GATOR1-SAMTOR complex
25GE 3.37 Å, Cryo-EM structure of the human KPTN-ITFG2-SZT2(2189-3432) complex
25GF 3.81 Å, Cryo-EM structure of the human KPTN-ITFG2-C12orf66-SZT2(2189-3432) complex
9O5E 5.0 Å, The dimeric KICSTOR-GATOR1 supercomplex

Browse structure collections

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