9W1J: Neurokinin A (NKA)-bound neurokinin 2 receptor
Cryo-EM structure of neurokinin A (NKA)-bound neurokinin 2 receptor (NK2R) in complex with miniGs/q70. Determined by electron microscopy at 2.97 Å resolution. Released 17 Jun 2026.
- Method
- Electron microscopy
- Resolution
- 2.97 Å
- Organisms
- Homo sapiens, Lama glama
- Chains
- 6
- Atoms
- 7,368
- Mol. weight
- 135.07 kDa
- Released
- 17 Jun 2026
Explore 9W1J in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9W1J contains 29 α-helices and 48 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 8 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-37 | 11 | |
| β-strand | 40-47 | 8 | 1 |
| α-helix | 53-55 | 3 | |
| β-strand | 208-214 | 7 | 1 |
| β-strand | 217-223 | 7 | 1 |
| α-helix | 235-238 | 4 | |
| β-strand | 243-249 | 7 | 1 |
| α-helix | 266-277 | 12 | |
| β-strand | 286-292 | 7 | 1 |
| α-helix | 294-302 | 9 | |
| α-helix | 313-315 | 3 | |
| α-helix | 334-349 | 16 | |
| β-strand | 359-363 | 5 | 1 |
| α-helix | 371-390 | 20 | |
Chain B: 2 helices, 29 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 15-25 | 11 | |
| α-helix | 30-33 | 4 | |
| β-strand | 43 | 1 | 5 |
| β-strand | 47-51 | 5 | 6 |
| β-strand | 58-63 | 6 | 7 |
| β-strand | 69-74 | 6 | 7 |
| β-strand | 78-83 | 6 | 7 |
| β-strand | 88-94 | 7 | 7 |
| β-strand | 100-105 | 6 | 8 |
| β-strand | 111-116 | 6 | 8 |
| β-strand | 121-125 | 5 | 8 |
| β-strand | 134-139 | 6 | 8 |
| β-strand | 146-151 | 6 | 9 |
| β-strand | 156-161 | 6 | 9 |
| β-strand | 165-170 | 6 | 9 |
| β-strand | 175-180 | 6 | 9 |
| β-strand | 187-192 | 6 | 10 |
| β-strand | 198-203 | 6 | 10 |
| β-strand | 208-212 | 5 | 10 |
| β-strand | 218-222 | 5 | 10 |
| β-strand | 229-234 | 6 | 11 |
| β-strand | 240-245 | 6 | 11 |
| β-strand | 250-254 | 5 | 11 |
| β-strand | 259-264 | 6 | 11 |
| β-strand | 273-278 | 6 | 5 |
| β-strand | 284-289 | 6 | 5 |
| β-strand | 294-298 | 5 | 5 |
| β-strand | 304-308 | 5 | 5 |
| β-strand | 315-320 | 6 | 6 |
| β-strand | 327-331 | 5 | 6 |
| β-strand | 336-339 | 4 | 6 |
Chain G: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-22 | 9 | |
| α-helix | 30-43 | 14 | |
Chain L: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-6 | 3 | |
Chain N: 1 helix, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 2 |
| β-strand | 19-25 | 7 | 2 |
| β-strand | 34-39 | 6 | 3 |
| β-strand | 45-51 | 7 | 3 |
| β-strand | 58-60 | 3 | 3 |
| β-strand | 68-69 | 2 | 2 |
| β-strand | 72-73 | 2 | 2 |
| β-strand | 78-83 | 6 | 2 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 3 |
| β-strand | 118 | 1 | 3 |
| β-strand | 122-124 | 3 | 3 |
Chain R: 15 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 32-60 | 29 | |
| α-helix | 70-81 | 12 | |
| α-helix | 82-89 | 8 | |
| α-helix | 90-94 | 5 | |
| α-helix | 112-136 | 25 | |
| α-helix | 145-162 | 18 | |
| β-strand | 170-174 | 5 | 4 |
| β-strand | 179-183 | 5 | 4 |
| α-helix | 192-201 | 10 | |
| α-helix | 202-207 | 6 | |
| α-helix | 208-226 | 19 | |
| α-helix | 239-270 | 32 | |
| α-helix | 279-281 | 3 | |
| α-helix | 285-297 | 13 | |
| α-helix | 298-300 | 3 | |
| α-helix | 302-308 | 7 | |
| α-helix | 313-320 | 8 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 229 | Homo sapiens | |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| Protachykinin-1 | L | protein | 11 | Homo sapiens | P20366 (AlphaFold model) |
| Nanobody 35 | N | protein | 134 | Lama glama | |
| Substance-K receptor | R | protein | 406 | Homo sapiens | P21452 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 358 | Homo sapiens | P62873 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>9W1J_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
GIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVD
KVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTI
SVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEF
LRISTASGDGRHYCYPHFTCAVDTENARRIFNDCKDIILQMNLREYNLV
Sequence of entity 2 (G), FASTA
>9W1J_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 3 (L), FASTA
>9W1J_3 Protachykinin-1 (chains L)
HKTDSFVGLMA
Sequence of entity 4 (N), FASTA
>9W1J_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHH
Sequence of entity 5 (R), FASTA
>9W1J_5 Substance-K receptor (chains R)
DYKDDDDAMGTCDIVTEANISSGPESNTTGITAFSMPSWQLALWATAYLALVLVAVTGNA
IVIWIILAHRRMRTVTNYFIVNLALADLCMAAFNAAFNFVYASHNIWYFGRAFCYFQNLF
PITAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPSTKAVIAGIWLVALALASPQCFYSTV
TMDQGATKCVVAWPEDSGGKTLLLYHLVVIALIYFLPLAVMFVAYSVIGLTLWRRAVPGH
QAHGANLRHLQAMKKFVKTMVLVVLTFAICWLPYHLYFILGSFQEDIYCHKFIQQVYLAL
FWLAMSSTMYNPIIYCCLNHRFRSGFRLAFRCCPWVTPTKEDKLELTPTTSLSTRVNRCH
TKETLFMAGDTAPSEATSGEAGRPQDGSGLWFGYGLLAPTKTHVEI
Sequence of entity 6 (B), FASTA
>9W1J_6 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Primary citation
Mechanistic basis for activation and selectivity of NK2R by endogenous and synthetic peptide agonists. Ren, Y., Pavlovskyil, A., Liu, X. et al. To be published.
Other PDB entries of the same protein (UniProt P59768 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
- 8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
- 6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
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