9W1J: Neurokinin A (NKA)-bound neurokinin 2 receptor

Cryo-EM structure of neurokinin A (NKA)-bound neurokinin 2 receptor (NK2R) in complex with miniGs/q70. Determined by electron microscopy at 2.97 Å resolution. Released 17 Jun 2026.

Method: Electron microscopy
Resolution: 2.97 Å
Organisms: Homo sapiens, Lama glama
Chains: 6
Atoms: 7,368
Mol. weight: 135.07 kDa
Released: 17 Jun 2026

Explore 9W1J in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9W1J contains 29 α-helices and 48 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	27-37	11
β-strand	40-47	8	1
α-helix	53-55	3
β-strand	208-214	7	1
β-strand	217-223	7	1
α-helix	235-238	4
β-strand	243-249	7	1
α-helix	266-277	12
β-strand	286-292	7	1
α-helix	294-302	9
α-helix	313-315	3
α-helix	334-349	16
β-strand	359-363	5	1
α-helix	371-390	20

Chain B: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	15-25	11
α-helix	30-33	4
β-strand	43	1	5
β-strand	47-51	5	6
β-strand	58-63	6	7
β-strand	69-74	6	7
β-strand	78-83	6	7
β-strand	88-94	7	7
β-strand	100-105	6	8
β-strand	111-116	6	8
β-strand	121-125	5	8
β-strand	134-139	6	8
β-strand	146-151	6	9
β-strand	156-161	6	9
β-strand	165-170	6	9
β-strand	175-180	6	9
β-strand	187-192	6	10
β-strand	198-203	6	10
β-strand	208-212	5	10
β-strand	218-222	5	10
β-strand	229-234	6	11
β-strand	240-245	6	11
β-strand	250-254	5	11
β-strand	259-264	6	11
β-strand	273-278	6	5
β-strand	284-289	6	5
β-strand	294-298	5	5
β-strand	304-308	5	5
β-strand	315-320	6	6
β-strand	327-331	5	6
β-strand	336-339	4	6

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	14-22	9
α-helix	30-43	14

Chain L: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	4-6	3

Chain N: 1 helix, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	2
β-strand	19-25	7	2
β-strand	34-39	6	3
β-strand	45-51	7	3
β-strand	58-60	3	3
β-strand	68-69	2	2
β-strand	72-73	2	2
β-strand	78-83	6	2
α-helix	88-90	3
β-strand	92-98	7	3
β-strand	118	1	3
β-strand	122-124	3	3

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	32-60	29
α-helix	70-81	12
α-helix	82-89	8
α-helix	90-94	5
α-helix	112-136	25
α-helix	145-162	18
β-strand	170-174	5	4
β-strand	179-183	5	4
α-helix	192-201	10
α-helix	202-207	6
α-helix	208-226	19
α-helix	239-270	32
α-helix	279-281	3
α-helix	285-297	13
α-helix	298-300	3
α-helix	302-308	7
α-helix	313-320	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	229	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Protachykinin-1	L	protein	11	Homo sapiens	P20366 (AlphaFold model)
Nanobody 35	N	protein	134	Lama glama
Substance-K receptor	R	protein	406	Homo sapiens	P21452 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	358	Homo sapiens	P62873 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9W1J_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
GIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVD
KVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTI
SVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEF
LRISTASGDGRHYCYPHFTCAVDTENARRIFNDCKDIILQMNLREYNLV

Sequence of entity 2 (G), FASTA

>9W1J_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (L), FASTA

>9W1J_3 Protachykinin-1 (chains L)
HKTDSFVGLMA

Sequence of entity 4 (N), FASTA

>9W1J_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHH

Sequence of entity 5 (R), FASTA

>9W1J_5 Substance-K receptor (chains R)
DYKDDDDAMGTCDIVTEANISSGPESNTTGITAFSMPSWQLALWATAYLALVLVAVTGNA
IVIWIILAHRRMRTVTNYFIVNLALADLCMAAFNAAFNFVYASHNIWYFGRAFCYFQNLF
PITAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPSTKAVIAGIWLVALALASPQCFYSTV
TMDQGATKCVVAWPEDSGGKTLLLYHLVVIALIYFLPLAVMFVAYSVIGLTLWRRAVPGH
QAHGANLRHLQAMKKFVKTMVLVVLTFAICWLPYHLYFILGSFQEDIYCHKFIQQVYLAL
FWLAMSSTMYNPIIYCCLNHRFRSGFRLAFRCCPWVTPTKEDKLELTPTTSLSTRVNRCH
TKETLFMAGDTAPSEATSGEAGRPQDGSGLWFGYGLLAPTKTHVEI

Sequence of entity 6 (B), FASTA

>9W1J_6 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Primary citation

Mechanistic basis for activation and selectivity of NK2R by endogenous and synthetic peptide agonists. Ren, Y., Pavlovskyil, A., Liu, X. et al. To be published.

Other PDB entries of the same protein (UniProt P59768 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor

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