Translocated intimin receptor Tir (tir) is a 550-residue protein from Escherichia coli O127:H6 (strain E2348/69 / EPEC). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: B7UM99.
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The mean pLDDT of this model is 44.6 (very low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 5% |
| 70 to 90 | Confident: backbone generally right | 23% |
| 50 to 70 | Low: treat with caution | 5% |
| Below 50 | Very low: often disordered regions | 67% |
What pLDDT means and how to read it
Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion. In strain E2348/69, acts mainly via the host adaptor proteins NCK1 and NCK2. Once clustered and phosphorylated at Tyr-474, Tir binds to NCK proteins, which in turn bind and activate host WASL/N-WASP, leading to actin polymerization. Can also trigger an…
Interacts with intimin. Interacts with host proteins NCK1, NCK2, alpha-actinin and BAIAP2
Secreted, Host cell membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 2CIA | X-ray | 1.45 Å | L=472-481 |
| 2CI9 | X-ray | 1.5 Å | L/M=470-481 |
| 5WEZ | X-ray | 2.74 Å | B=32-80 |
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