Rqc2 homolog RqcH (rqcH) is a 570-residue protein from Bacillus subtilis (strain 168). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: O34693.
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The mean pLDDT of this model is 91.1 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 72% |
| 70 to 90 | Confident: backbone generally right | 25% |
| 50 to 70 | Low: treat with caution | 2% |
| Below 50 | Very low: often disordered regions | 1% |
What pLDDT means and how to read it
Key component of the ribosome quality control system (RQC), a ribosome-associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes and their subsequent degradation (PubMed:31155236). RqcH recruits Ala-charged tRNA, and with RqcP directs the elongation of stalled nascent chains on 50S ribosomal subunits, leading to non-templated C-terminal alanine extensions (Ala tail) (PubMed:31155236, PubMed:35264791, PubMed:38177497). The Ala tail promotes nascent chain degradation (PubMed:31155236). RqcH, RqcP and charged tRNA(Ala) are necessary and sufficient to add an Ala tail to a model stalled nascent peptide; does not add Val (PubMed:34255840).…
Associates with isolated or stalled 50S ribosomal subunits (PubMed:31155236, PubMed:33259811, PubMed:33259810, PubMed:34255840). Binds to RqcP (PubMed:33259811, PubMed:33259810, PubMed:34255840). Interacts with ribosomal protein uL11 (PubMed:33259811, PubMed:33259810, PubMed:34255840). Displaced from the 50S subunit by thiostrepton (PubMed:33259810, PubMed:34255840). In crystallized 50S subunits…
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 7AS8 | EM | 2.9 Å | 0=1-570 |
| 7AQC | EM | 2.99 Å | R=1-570 |
| 7AQD | EM | 3.1 Å | R=1-570 |
| 7OPE | EM | 3.2 Å | 0=1-570 |
| 8S1U | EM | 3.4 Å | H=1-570 |
| 7AS9 | EM | 3.5 Å | 0=1-570 |
| 7ASA | EM | 3.5 Å | 0=1-570 |
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