SH2/SH3 adapter protein NCK1 (NCK1) is a 377-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P16333.
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The mean pLDDT of this model is 70.8 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 14% |
| 70 to 90 | Confident: backbone generally right | 52% |
| 50 to 70 | Low: treat with caution | 10% |
| Below 50 | Very low: often disordered regions | 24% |
What pLDDT means and how to read it
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors
Interacts (via SH2 domain and SH3 domain 2) with EGFR. Interacts with PAK1 and SOS1. Interacts (via SH3 domains) with PKN2. Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. This interaction is required for nuclear import. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1. Interacts with CAV2 (tyrosine…
Cytoplasm, Endoplasmic reticulum, Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5QU8 | X-ray | 0.93 Å | A=1-61 |
| 5QU3 | X-ray | 1.02 Å | A/B=4-59 |
| 5QU2 | X-ray | 1.04 Å | A/B=1-59 |
| 5QU4 | X-ray | 1.05 Å | A/B/C/D=1-61 |
| 5QU1 | X-ray | 1.08 Å | A/B=4-59 |
| 5QU5 | X-ray | 1.11 Å | A/B=1-61 |
| 5QU7 | X-ray | 1.27 Å | A/B=4-59 |
| 2CI9 | X-ray | 1.5 Å | A/B=281-377 |
| 5QUA | X-ray | 1.51 Å | A/B=1-61 |
| 2CI8 | X-ray | 1.8 Å | A=281-377 |
| 5QU6 | X-ray | 1.82 Å | 1/2/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z=1-61 |
| 2CUB | NMR | A=99-173 | |
| 2JS0 | NMR | A=107-165 | |
| 2JS2 | NMR | A=1-61 | |
| 2JW4 | NMR | A=1-63 |
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