Serine/threonine-protein kinase RAD53 (RAD53) is a 821-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P22216.
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The mean pLDDT of this model is 72.9 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 36% |
| 70 to 90 | Confident: backbone generally right | 30% |
| 50 to 70 | Low: treat with caution | 9% |
| Below 50 | Very low: often disordered regions | 25% |
What pLDDT means and how to read it
Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1
Interacts (via domain FHA 1) with PTC2 (when phosphorylated); the interaction is direct and serves to regulate DNA damage checkpoint signaling (PubMed:12667463). Interacts with PIN4 (PubMed:15024067)
Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 1G6G | X-ray | 1.6 Å | A/B=29-155 |
| 5T2S | X-ray | 2.4 Å | A/C=22-162 |
| 4PDP | X-ray | 2.59 Å | A/B=170-512 |
| 6C4U | X-ray | 2.6 Å | A/B/C/D/E/F=29-162 |
| 5T2F | X-ray | 2.66 Å | A/B/C/D=22-162 |
| 5XZW | X-ray | 2.8 Å | A/B=1-466 |
| 4PDS | X-ray | 2.9 Å | A/B=170-512 |
| 2YGV | X-ray | 2.94 Å | E/F/G/H=800-821 |
| 5XZV | X-ray | 3.1 Å | A/B=1-466 |
| 1DMZ | NMR | A=573-730 | |
| 1FHQ | NMR | A=573-730 | |
| 1FHR | NMR | A=573-730 | |
| 1G3G | NMR | A=1-164 | |
| 1J4K | NMR | A=573-730 | |
| 1J4L | NMR | A=573-730 | |
| 1J4O | NMR | A=14-164 | |
| 1J4P | NMR | A=14-164 | |
| 1J4Q | NMR | A=14-164 | |
| 1K2M | NMR | A=573-730 | |
| 1K2N | NMR | A=573-730 |
Showing 20 of 27 experimental structures (best resolution first).
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