Spindle assembly checkpoint protein BUB3 (BUB3) is a 341-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P26449.
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The mean pLDDT of this model is 93.4 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 84% |
| 70 to 90 | Confident: backbone generally right | 12% |
| 50 to 70 | Low: treat with caution | 4% |
| Below 50 | Very low: often disordered regions | 0% |
What pLDDT means and how to read it
Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments (PubMed:15879521, PubMed:24066227). Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 (PubMed:15879521)
Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20 (PubMed:11726501, PubMed:15879521). Part of complex consisting of MAD1, BUB1 and BUB3 after activation of spindle checkpoint (PubMed:10837255, PubMed:17227844). Part of the BUB1-BUB3 complex, composed of BUB1 and BUB3 (PubMed:24066227). Interacts with SPC105 (via phosphorylated MELT motifs); the…
Nucleus, Chromosome, centromere, kinetochore
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 1YFQ | X-ray | 1.1 Å | A=1-341 |
| 2I3S | X-ray | 1.9 Å | A/C/E=1-341 |
| 4BL0 | X-ray | 1.95 Å | A/D=1-341 |
| 1U4C | X-ray | 2.35 Å | A/B=1-341 |
| 2I3T | X-ray | 2.8 Å | A/C/E/G=1-341 |
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