FACT complex subunit SPT16 (SPT16) is a 1035-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P32558.
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The mean pLDDT of this model is 83.3 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 59% |
| 70 to 90 | Confident: backbone generally right | 24% |
| 50 to 70 | Low: treat with caution | 4% |
| Below 50 | Very low: often disordered regions | 13% |
What pLDDT means and how to read it
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription…
Forms a stable heterodimer with POB3 (PubMed:10413469, PubMed:9705338, PubMed:9832518). The SPT16-POB3 dimer weakly associates with multiple molecules of NHP6 (NHP6A or NHP6B) to form the FACT (yFACT or SNP) complex (PubMed:11313475, PubMed:11432837, PubMed:12952948). The FACT complex interacts with the CK2 (casein kinase II) complex subunits CKA1, CKA2, CKB1 and CKB2 and the components of the…
Nucleus, Chromosome
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3BIQ | X-ray | 1.73 Å | A=1-465 |
| 4WNN | X-ray | 1.8 Å | T=957-972 |
| 3BIT | X-ray | 1.9 Å | A/B=1-451 |
| 3BIP | X-ray | 1.94 Å | A/B=1-465 |
| 4IOY | X-ray | 1.94 Å | X=675-958 |
| 7NKY | EM | 3.2 Å | Q=1-1035 |
| 8XGC | EM | 3.7 Å | L=1-1035 |
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