Ubiquitin carboxyl-terminal hydrolase 8 (USP8) is a 1118-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P40818.
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The mean pLDDT of this model is 71.3 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 36% |
| 70 to 90 | Confident: backbone generally right | 28% |
| 50 to 70 | Low: treat with caution | 6% |
| Below 50 | Very low: often disordered regions | 30% |
What pLDDT means and how to read it
Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein…
Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ (By similarity). Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules (By similarity). Interacts with BIRC6/bruce…
Cytoplasm, Nucleus, Endosome membrane, Cell membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 6F09 | X-ray | 1.59 Å | A/B/C/D=712-724 |
| 8ADM | X-ray | 1.7 Å | P=715-722 |
| 2GFO | X-ray | 2.0 Å | A=734-1110 |
| 2A9U | X-ray | 2.1 Å | A/B=1-142 |
| 8XPN | X-ray | 2.1 Å | A/B/C=734-1110 |
| 2GWF | X-ray | 2.3 Å | A/C/E=181-318 |
| 3N3K | X-ray | 2.6 Å | A=734-1110 |
| 8Y9A | X-ray | 3.1 Å | A=1-142 |
| 1WHB | NMR | A=174-317 |
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