Biotin biosynthesis cytochrome P450 (bioI) is a 395-residue protein from Bacillus subtilis (strain 168). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P53554.
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The mean pLDDT of this model is 93.8 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 85% |
| 70 to 90 | Confident: backbone generally right | 12% |
| 50 to 70 | Low: treat with caution | 3% |
| Below 50 | Very low: often disordered regions | 0% |
What pLDDT means and how to read it
Catalyzes the C-C bond cleavage of fatty acid linked to acyl carrier protein (ACP) to generate pimelic acid for biotin biosynthesis. It has high affinity for long-chain fatty acids with the greatest affinity for myristic acid
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3EJB | X-ray | 2.0 Å | B/D/F/H=2-395 |
| 3EJD | X-ray | 2.1 Å | B/D/F/H=2-395 |
| 3EJE | X-ray | 2.1 Å | B/D/F/H=2-395 |
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