Intermembrane phospholipid transport system binding protein MlaD (mlaD) is a 183-residue protein from Escherichia coli (strain K12). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P64604.
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The mean pLDDT of this model is 84.9 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 63% |
| 70 to 90 | Confident: backbone generally right | 16% |
| 50 to 70 | Low: treat with caution | 9% |
| Below 50 | Very low: often disordered regions | 12% |
What pLDDT means and how to read it
Part of the ABC transporter complex MlaFEDB, which is involved in a phospholipid transport pathway that maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane (PubMed:19383799, PubMed:27529189). MlaD functions in substrate binding with strong affinity for phospholipids and modulates ATP hydrolytic activity of the complex (PubMed:27529189)
The complex is composed of two ATP-binding proteins (MlaF), two transmembrane proteins (MlaE), two cytoplasmic solute-binding proteins (MlaB) and six periplasmic solute-binding proteins (MlaD) (PubMed:27529189, PubMed:28388411). Interacts with MlaC (PubMed:28388411)
Cell inner membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5UW8 | X-ray | 2.15 Å | A/B/C/D/E/F/G=32-140 |
| 8HPZ | X-ray | 2.3 Å | A/B/C=29-183 |
| 8HQ9 | X-ray | 2.7 Å | A/B/C/D/E/F=29-183 |
| 5UW2 | X-ray | 2.85 Å | A/B/C=32-183 |
| 7CGE | EM | 2.9 Å | G/H/I/J/K/L=1-183 |
| 8HQA | X-ray | 3.2 Å | A/B/C=29-183 |
| 6ZY9 | EM | 3.3 Å | A/D/I/J/K/L=1-183 |
| 7CH0 | EM | 3.7 Å | G/H/I/J/K/L=1-183 |
| 7CGN | EM | 4.3 Å | G/H/I/J/K/L=1-183 |
| 8OJ4 | EM | 4.35 Å | A/B/C/D/E/F=1-183 |
| 8OJG | EM | 4.38 Å | A/B/C/D/E/F=1-183 |
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