Mitochondrial import inner membrane translocase subunit TIM44 (TIM44) is a 431-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q01852.
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The mean pLDDT of this model is 70.4 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 20% |
| 70 to 90 | Confident: backbone generally right | 41% |
| 50 to 70 | Low: treat with caution | 20% |
| Below 50 | Very low: often disordered regions | 19% |
What pLDDT means and how to read it
Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 and its co-chaperone (MGE1) to drive protein translocation into the matrix using ATP as an energy source (PubMed:10548718, PubMed:1396562, PubMed:18426906, PubMed:26714107, PubMed:7600576, PubMed:9171336). Tim44 is an essential, peripheral membrane protein in TIM23 complex. Tim44 is tightly associated with the inner mitochondrial membrane on the matrix side and the TIM44 C-terminal domain (CTD) functions as an inner mitochondrial membrane (IMM) anchor that…
Component of the PAM complex, at least composed of SSC1 (mtHsp70), MGE1, TIM44, PAM16/TIM16, PAM17 and PAM18/TIM14. Forms part of the receptor complex that consists of at least 3 different proteins (TIM17, TIM23, TIM44) (PubMed:10548718, PubMed:16107694, PubMed:18426906, PubMed:26714107, PubMed:7600576, PubMed:9171336). The TIM23 complex interacts with the TOM complex to form a supercomplex…
Mitochondrion inner membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 8E1M | EM | 2.9 Å | C=1-431 |
| 3QK9 | X-ray | 3.1 Å | A/B=210-431 |
| 2FXT | X-ray | 3.2 Å | A=234-425 |
| 9J9B | EM | 3.83 Å | C=1-431 |
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